Doubled haploid lines of <i>Brassica carinata</i> with modified erucic acid content through mutagenesis by EMS treatment of isolated microspores

Barro, Francisco; Fernández‐Escobar, J.; Vega, María Isabel Álvarez; Martı́n, A.

doi:10.1046/j.1439-0523.2001.00602.x

Cited by 57 publications

(28 citation statements)

References 20 publications

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“…B. napus doubled haploid plants regenerated from microspores exposed to UV light exhibited changes in the level of saturated fatty acids. Differences in erucic acid levels and glucosinolate levels of B. carinata DH lines were reported after the microspores had been subjected to UV light or EMS (Barro et al 2001(Barro et al , 2002. Valuable agronomic characteristics have also been observed in DH plants regenerated from microspores exposed to UV (McClinchey and Kott 2008).…”

Section: Utilization Of Doubled Haploids In Mutagenesismentioning

confidence: 99%

Haploids and doubled haploids in Brassica spp. for genetic and genomic research

Ferrie

Möllers

2010

Plant Cell Tiss Organ Cult

103

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The availability of a highly efficient and reliable microspore culture protocol for many Brassica species makes this system useful for studying basic and applied research questions. Microspores and microspore-derived embryos are ideal targets for modification by mutagenesis and transformation. Regenerated doubled haploid plants are widely used in breeding programs and in genetic studies. Furthermore, the Brassica microspore culture system allows the identification of genomic regions and genes involved in the microspore embryogenic response, spontaneous diploidization and direct embryo to plant conversion. This review summarizes current achievements and discusses future perspectives.

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Section: Utilization Of Doubled Haploids In Mutagenesismentioning

confidence: 99%

Haploids and doubled haploids in Brassica spp. for genetic and genomic research

Ferrie

Möllers

2010

Plant Cell Tiss Organ Cult

103

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“…Traits are fixed and homozygous true breeding lines are produced within one generation, which can help reduce the time needed to develop new varieties (Thomas et al 2003;Ferrie and Caswell 2011;Ferrie and Möllers 2011). For the same reasons, in vitro microspore culture has become an important tool for mutagenesis (Barro et al 2001(Barro et al , 2002Sonntag and Rudloff 2004;Liu et al 2005;Ferrie et al 2008) and in vitro selection (Albrecht et al 1995;Möllers et al 2000;Liu et al 2005). In addition, microspores, MDEs and DH plants are being used for transformation studies.…”

Section: Introductionmentioning

confidence: 99%

Identification and evaluation of intervarietal substitution lines of rapeseed (Brassica napus L.) with donor segments affecting the diploidization rate of isolated microspores

et al. 2016

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In Brassica species microspore derived doubled haploid lines are an important tool in breeding and research. A limiting step in the production of doubled haploid lines is the diploidization of the microspores. Strong differences have been observed in diploidization rates between different genotypes but little is know about the genetic control of these differences. To identify genomic regions that carry genetic factors controlling the diploidization rate of isolated microspores of rapeseed, marker segregations were compared between segregating populations of diploid and haploid microspore derived embryos and a BC 1 from a cross between 'Express 617' and 'RS239'. After map construction ten intervarietal substitution lines from the same cross were selected with donor segments covering five genomic regions that showed a pattern of skewed marker segregations across the three populations indicative of the segregation of genes controlling the diploidization rates. The diploidization rates of microspores of the ten lines ranged from 23.9 to 58.7 % while the recurrent parent 'Express 617' showed a rate of 52.5 %. For three lines the diploidization rates were significantly lower (P = 0.05) than the rate of 'Express 617'. By comparing donor segments between the significant and the non-significant lines, seven genomic regions that cover just between 4.17 and 6.16 % of the rapeseed genome were identified that may contain genetic factors controlling diploidization rates in rapeseed. In addition, one marker was found that has a high probability to be linked to such a factor. The significant lines represent an ideal material for further in depth studies of this trait.

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“…Many rapeseed varieties currently grown are hybrids of DH lines. Also, microspores and MDEs can be used for mutagenesis and in vitro selection (Albrecht et al 1995;Barro et al 2001;Ferrie et al 2008;McClinchey and Kott 2008) and it is possible to utilize microspores for genetic transformation (Guerche et al 1987;Fukuoka et al 1998;Nehlin et al 2000;Dormann et al 2001). The main advantage of androgenesis is, that traits can be fixed in one generation rather than after multiple selfpollinated generations (Dunwell 2010;Ferrie and Caswell 2011;Germanà 2011).…”

Section: Introductionmentioning

confidence: 99%

Identification and evaluation of intervarietal substitution lines of rapeseed (Brassica napus L.) with donor segments affecting the direct embryo to plant conversion rate of microspore-derived embryos

et al. 2016

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Microspore culture has become an important tool in many species, including Brassicas, for the production of entirely homozygous lines, so called double haploid (DH) lines. The primary products of microspore culture are embryo-like structures, called microspore-derived embryos (MDEs). A major problem in the development of DH lines is the often low efficiency of Direct Embryo to Plant Conversion (DEPC). During the development of DH populations, favourable alleles of genes affecting the DEPC rate will be under selection. This selection should lead to skewed segregations at markers linked to these genes. By comparing skewed marker segregations in four populations, a population of doubled haploid plantlets, a haploid and a doubled haploid MDE population, and a BC 1 population, 20 genomic regions were identified, which showed patterns of skewed segregations across the populations, indicative of the segregation of genetic factors controlling DEPC rates. Four regions and eight intervarietal substitution lines (ISLs) with donor segments overlapping these regions were selected for further studies. Three ISLs, ER654, ER661 and ER653 with DEPC rates of 49.1, 54.5 and 57.2 %, showed significantly reduced DEPC rates compared to the rate of the recurrent parent of 76.5 %. By comparing donor segments between the significant and the non-significant lines, eight genomic regions were identified that may contain genetic factors controlling the DEPC rate in rapeseed. These regions range in size from 0 (represented by just one marker) to 16.5 cM and cover together just 1.33 % of the genetic map used to characterize the donor segments in the ISLs.

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Doubled haploid lines of Brassica carinata with modified erucic acid content through mutagenesis by EMS treatment of isolated microspores

Cited by 57 publications

References 20 publications

Haploids and doubled haploids in Brassica spp. for genetic and genomic research

Haploids and doubled haploids in Brassica spp. for genetic and genomic research

Identification and evaluation of intervarietal substitution lines of rapeseed (Brassica napus L.) with donor segments affecting the diploidization rate of isolated microspores

Identification and evaluation of intervarietal substitution lines of rapeseed (Brassica napus L.) with donor segments affecting the direct embryo to plant conversion rate of microspore-derived embryos

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