Down‐regulated HDAC3 elevates microRNA‐495‐3p to restrain epithelial‐mesenchymal transition and oncogenicity of melanoma cells via reducing TRAF5

Abstract

MicroRNAs (miRNAs) are emerging biomarkers in biological processes and the role of miR‐495‐3p has been identified in melanoma, while the detailed molecular mechanisms remain to be further explored. We aim to explore the effect of histone deacetylase 3 (HDAC3) and miR‐495‐3p on epithelial‐mesenchymal transition (EMT) and oncogenicity of melanoma cells by regulating tumour necrosis factor receptor‐associated factor 5 (TRAF5). Levels of HDAC3, miR‐495‐3p and TRAF5 in melanoma tissues and pigmented nevus tissues were determined, and the predictive roles of HDAC3 and miR‐495‐3p in prognosis of melanoma patients were measured. The melanoma cells were screened and transfected with relative oligonucleotides and plasmids, and the expression of HDAC3, miR‐495‐3p and TRAF5, and phenotypes of melanoma cells were gauged by a series of assays. The relations between HDAC3 and miR‐495‐3p, and between miR‐495‐3p and TRAF5 were confirmed. HDAC3 and TRAF5 were increased while miR‐495‐3p was decreased in melanoma cells and tissues, and the low expression of miR‐495‐3p as well as high expression of HDAC3 indicated a poor prognosis of melanoma patients. Inhibited HDAC3 elevated miR‐495‐3p to suppress EMT and oncogenicity of melanoma cells by reducing TRAF5. HDAC3 particularly bound to miR‐495‐3p and TRAF5 was the target gene of miR‐495‐3p. Our results revealed that down‐regulated HDAC3 elevates miR‐495‐3p to suppress malignant phenotypes of melanoma cells by inhibiting TRAF5, thereby repressing EMT progression of melanoma cells. This study may provide novel targets for melanoma treatment.

Development and Validation of a Necroptosis-Related Prognostic Model in Head and Neck Squamous Cell Carcinoma

“…As the regulatory gene with the smallest hazard ratio (HR) (0.732) in our study, TAR5 has been studied in many cancers. Previous studies have demonstrated that TAR5 is a key target to inhibit tumor cell migration or proliferation, and the expression of TRAF negatively correlates with prognosis [25][26][27]. However, in this study, we obtained the opposed conclusion that TAR5 is a protective factor for HNSCC, and TAR5 expression contributed to a longer OS time.…”

MicroRNA-495/TGF-β/FOXC1 axis regulates multidrug resistance in metaplastic breast cancer cells

“…Although miR expression profile in metaplastic breast cancer is poorly studied, as most studies pool them within the TNBC group, another miR family, which is downregulated in most TNBC, including metaplastic, is the miR-200 family [81,82]. In gastric carcinoma and melanoma, miR-495-3p is down-regulated and associated with poor disease prognosis [83,84]. Although no reports have studied miR-495-3p expression exclusively in metaplastic breast cancer, it is important to note that miR-200a targets FOXC1 [85], which is upregulated in metaplastic breast cancer.…”

Effect of Space Flight on the Behavior of Human Retinal Pigment Epithelial ARPE-19 Cells and Evaluation of Coenzyme Q10 Treatment

“…Relevant, other microRNAs we identified are involved in the EMT process. For example, hsa-miR-145-3p, hsa-miR-324-5p and hsa-miR142-5p act as inhibitors of EMT in different cell types [81][82][83][84]; on the contrary, high expression and activation of hsa-miR 483-3p, hsa-miR-128-3p are involved in EMT induction [85,86].…”