Down regulation of a heavy metal transporter gene influences several domestication traits and grain Fe-Zn content in rice

Kappara, Saivishnupriya; Neelamraju, Sarla; Ramanan, Rajeshwari

doi:10.1016/j.plantsci.2018.09.003

Cited by 24 publications

(14 citation statements)

References 54 publications

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“…Kappara et al [28] identified two alleles of the OsHMA7 transporter gene in rice by using QTL (quantitative trait locus) and analyzing lines which accumulated high and low levels of iron and zinc. The authors found that the overexpression of allele 284 in transgenic rice lines resulted in a high accumulation of iron and zinc, as well as tolerance to iron- and zinc-deficient soils.…”

Section: Methods Of Biofortificationmentioning

confidence: 99%

Biotechnological Approaches for Generating Zinc-Enriched Crops to Combat Malnutrition

Hefferon

2019

Nutrients

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The past twenty years have seen the application of biotechnology to generate nutritionally improved food crops. Biofortified rice, cassava, maize, sorghum and other staple crops biofortified with essential micronutrients have great potential to benefit the world’s poor, in terms of both health and economics. This paper describes the use of genetic modification to generate crops that are biofortified with zinc. Examples of zinc-enhanced crops which have been developed using biotechnological approaches will be discussed, and new approaches for research and development will be outlined. The impact of these biofortified crops on human health and well-being will be examined. This paper will conclude with a discussion of the obstacles that must be overcome to enable zinc-fortified crops to be accessible for the world’s malnourished.

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Section: Methods Of Biofortificationmentioning

confidence: 99%

Biotechnological Approaches for Generating Zinc-Enriched Crops to Combat Malnutrition

Hefferon

2019

Nutrients

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“…Kappara et al . (2018) reported that knocking down OsHMA7‐like by RNAi resulted in increased sensitivity to Zn and Fe deficiencies. The biological functions of OsHMA20‐like , OsHMA28‐like and OsHMA33‐like have not been reported, although they have been predicted to have roles in metal transport.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome profile analysis of rice varieties with different tolerance to zinc deficiency

et al. 2021

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Zinc (Zn) is an indispensable element for rice growth. Zn deficiency results in brown blotches and streaks 2–3 weeks after transplanting, as well as stunting, reduced tillering, and low productivity of rice plants. These processes are controlled by different families of expressed genes. A comparative transcriptome profile analysis was conducted using the roots of two Zn deficiency tolerant varieties (UCP122 and KALIBORO26) and two sensitive varieties (IR26 and IR64) by merging data from untreated control (CK) and Zn deficiency treated samples. Results revealed a total of 4,688 differentially expressed genes (DEGs) between the normal Zn and deficient conditions, with 2,702 and 1,489 unique DEGs upregulated and downregulated, respectively. Functional enrichment analysis identified transcription factors (TFs), such as WRKY, MYB, ERF, and bHLH which are important in the regulation of the Zn deficiency response. Furthermore, chitinases, jasmonic acid, and phenylpropanoid pathways were found to be important in the Zn deficiency response. The metal tolerance protein (MTP) genes also appeared to play an important role in conferring tolerance to Zn deficiency. A heavy metal‐associated domain‐containing protein 7 was associated with tolerance to Zn deficiency and negatively regulated downstream genes. Collectively, our findings provide valuable expression patterns and candidate genes for the study of molecular mechanisms underlying the response to Zn deficiency and for improvements in breeding for tolerance to Zn deficiency in rice.

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“…Finally, we find that OsHMA7 is within a QTL. The OsHMA7 allelic variation was analyzed in recombinant inbred lines generated from crosses between Madhukar × Swarna, which show high and low Fe concentration in seeds, respectively ( Kappara et al, 2018 ). Results show that lines silenced for OsHMA7 or overexpressing either alleles result in complex phenotypes with changes in plant size and domestication traits.…”

Section: Possible Candidate Genes With Known Function Within Qtl Regionsmentioning

confidence: 99%

“…Results show that lines silenced for OsHMA7 or overexpressing either alleles result in complex phenotypes with changes in plant size and domestication traits. However, the over-expression of the allele from the high-Fe genotype results in increased Fe concentration in seeds, whereas the overexpression of the allele from the low-Fe genotype did not ( Kappara et al, 2018 ). Therefore, OsHMA7 , despite not having its molecular function characterized yet, is a good candidate gene for natural variation in Fe levels in rice grains.…”

Section: Possible Candidate Genes With Known Function Within Qtl Regionsmentioning

confidence: 99%

Iron Biofortification in Rice: An Update on Quantitative Trait Loci and Candidate Genes

et al. 2021

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Rice is the most versatile model for cereals and also an economically relevant food crop; as a result, it is the most suitable species for molecular characterization of Fe homeostasis and biofortification. Recently there have been significant efforts to dissect genes and quantitative trait loci (QTL) associated with Fe translocation into rice grains; such information is highly useful for Fe biofortification of cereals but very limited in other species, such as maize (Zea mays) and wheat (Triticum aestivum). Given rice’s centrality as a model for Poaceae species, we review the current knowledge on genes playing important roles in Fe transport, accumulation, and distribution in rice grains and QTLs that might explain the variability in Fe concentrations observed in different genotypes. More than 90 Fe QTLs have been identified over the 12 rice chromosomes. From these, 17 were recorded as stable, and 25 harbored Fe-related genes nearby or within the QTL. Among the candidate genes associated with Fe uptake, translocation, and loading into rice grains, we highlight the function of transporters from the YSL and ZIP families; transporters from metal-binding molecules, such as nicotianamine and deoxymugineic acid; vacuolar iron transporters; citrate efflux transporters; and others that were shown to play a role in steps leading to Fe delivery to seeds. Finally, we discuss the application of these QTLs and genes in genomics assisted breeding for fast-tracking Fe biofortification in rice and other cereals in the near future.

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Down regulation of a heavy metal transporter gene influences several domestication traits and grain Fe-Zn content in rice

Cited by 24 publications

References 54 publications

Biotechnological Approaches for Generating Zinc-Enriched Crops to Combat Malnutrition

Biotechnological Approaches for Generating Zinc-Enriched Crops to Combat Malnutrition

Comparative transcriptome profile analysis of rice varieties with different tolerance to zinc deficiency

Iron Biofortification in Rice: An Update on Quantitative Trait Loci and Candidate Genes

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