Down-regulation of a Phaseolus vulgaris annexin impairs rhizobial infection and nodulation

Carrasco-Castilla, Janet; Ortega-Ortega, Yolanda; Jáuregui-Zúñiga, David; Juárez-Verdayes, Marco A.; Arthikala, Manoj‐Kumar; Monroy-Morales, Elizabeth; Nava, Noreide; Santana, Olivia; Sánchez‐López, Rosana; Quinto, Carmen

doi:10.1016/j.envexpbot.2018.05.016

Cited by 16 publications

(12 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The stimulation of Ca 2+ ion transfer through the plasma membrane and ROS production caused by Nod factors constitute an early response in the signal transduction pathway. Analysis of PvAnn1RNAi transgenic roots inocu lated with rhizobia showed a decrease in ROS production and Ca 2+ influx into the cells, which resulted in impaired progression and decreased numbers of infection threads and nodules (CarrascoCastilla et al, 2018). Taken together, these findings point to the involvement of PvAnn1 in the regulation of signal transduction at early stages.…”

Section: Introductionmentioning

confidence: 77%

“…Another annexin, MtAnn3 (Medtr4g097180), was found to be important for root hair deformations in M. truncatula (Gong et al, 2012). At the same time, close homologs of MtAnn1 -PvAnn1 (Phvul.011g209300) and LjAnn1 (Lj0g3v0203419), which belong to the same phylogenetic group as MtAnn1, play important roles in the symbiotic process in P. vulgaris and L. japonicus (Wienkoop, Saalbach, 2003;JáureguiZúñiga et al, 2016;CarrascoCastilla et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…The search of the sequences presumably coding for annexins in legumes was performed using BlastX with 8 pre vious ly revealed M. truncatula and 13 P. vulgaris nucleotide sequences encoding these proteins (Kodavali et al, 2013;CarrascoCastilla et al, 2018) as queries against different plant sequence databases: https://phytozome.jgi.doe.gov/pz/ portal.html for M. truncatula and P. vulgaris, http://www. kazusa.or.jp/lotus/ for L. japonicus, and the URGI data base v. 1 https://urgi.versailles.inra.fr/blast for P. sativum L. (Clark et al, 2001;CarrascoCastilla et al, 2018;Kreplak et al, 2019). As a result, we were able to identify 18 cod ing sequences (CDSs) for annexins in M. truncatula, 15 in P. sativum L., and 13 in L. japonicus (Table 2).…”

Section: Phylogenetic Analysis Of Annexins In Pea and Other Legumesmentioning

confidence: 99%

“…Twentythree genes had been previously found to encode annexins in soy bean (Feng et al, 2013). The coding sequences for annexins from P. sativum were named based on their phylogenetic relationships with the corresponding homologous sequences from M. truncatula and P. vulgaris (see Table 2) (Clark et al, 2012;Kodavali et al, 2013;CarrascoCastilla et al, 2018).…”

Section: Phylogenetic Analysis Of Annexins In Pea and Other Legumesmentioning

confidence: 99%

“…A protein homologous to MtAnn1 -PvAnn1 from Phaseo lus vulgaris -is activated at the early stages of symbiosis development (JáureguiZúñiga et al, 2016;CarrascoCastilla et al, 2018). The stimulation of Ca 2+ ion transfer through the plasma membrane and ROS production caused by Nod factors constitute an early response in the signal transduction pathway.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Phylogenetic and structural analysis of annexins in pea (<i>Pisum sativum</i> L.) and their role in legume-rhizobial symbiosis development

et al. 2021

View full text Add to dashboard Cite

Annexins as Ca2+/phospholipid-binding proteins are involved in the control of many biological processes essential for plant growth and development. In a previous study, we had shown, using a proteomic approach, that the synthesis of two annexins is induced in pea roots in response to rhizobial inoculation. In this study, phylogenetic analysis identified these annexins as PsAnn4 and PsAnn8 based on their homology with annexins from other legumes. The modeling approach allowed us to estimate the structural features of these annexins that might influence their functional activity. To verify the functions of these annexins, we performed comparative proteomic analysis, experiments with calcium influx inhibitors, and localization of labeled proteins. Essential down-regulation of PsAnn4 synthesis in a non-nodulating pea mutant P56 (sym10) suggests an involvement of this annexin in the rhizobial symbiosis. Quantitative RT-PCR analysis showed that PsAnn4 was upregulated at the early stages of symbiosis development, starting from 1–3 days after inoculation to up to 5 days after inoculation, while experiments with the Ca2+ channel blocker LaCl3 revealed its negative influence on this expression. To follow the PsAnn4 protein localization in plant cells, it was fused to the fluorophores such as red fluorescent protein (RFP) and yellow fluorescent protein (YFP) and expressed under the transcriptional regulation of the 35S promoter in Nicotiana benthamiana leaves by infiltration with Agrobacterium tumefaciens. The localization of PsAnn4 in the cell wall or plasma membrane of plant cells may indicate its participation in membrane modification or ion transport. Our results suggest that PsAnn4 may play an important role during the early stages of pea-rhizobial symbiosis development.

show abstract

Section: Introductionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Phylogenetic Analysis Of Annexins In Pea and Other Legumesmentioning

confidence: 99%

Section: Phylogenetic Analysis Of Annexins In Pea and Other Legumesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Phylogenetic and structural analysis of annexins in pea (<i>Pisum sativum</i> L.) and their role in legume-rhizobial symbiosis development

et al. 2021

View full text Add to dashboard Cite

show abstract

Common Bean Genetics, Breeding, and Genomics for Adaptation to Changing to New Agri-environmental Conditions

Ron

Kalavacharla

Álvarez-García

et al. 2019

Genomic Designing of Climate-Smart Pulse Crops

View full text Add to dashboard Cite

Rhizobium etli CE3-DsRed pMP604: a useful biological tool to study initial infection steps in Phaseolus vulgaris nodulation

Dávila-Delgado

Bañuelos-Vazquez

Monroy-Morales

et al. 2020

Planta

View full text Add to dashboard Cite

Down-regulation of a Phaseolus vulgaris annexin impairs rhizobial infection and nodulation

Cited by 16 publications

References 72 publications

Phylogenetic and structural analysis of annexins in pea (<i>Pisum sativum</i> L.) and their role in legume-rhizobial symbiosis development

Phylogenetic and structural analysis of annexins in pea (<i>Pisum sativum</i> L.) and their role in legume-rhizobial symbiosis development

Common Bean Genetics, Breeding, and Genomics for Adaptation to Changing to New Agri-environmental Conditions

Rhizobium etli CE3-DsRed pMP604: a useful biological tool to study initial infection steps in Phaseolus vulgaris nodulation

Contact Info

Product

Resources

About