Down-regulation of Bcl-2 and Akt induced by combination of photoactivated hypericin and genistein in human breast cancer cells

Ferenc, Peter; Solár, Peter; Kleban, Ján; Mikeš, Jaromír; Fedoročko, Peter

doi:10.1016/j.jphotobiol.2009.10.004

Cited by 61 publications

(46 citation statements)

References 51 publications

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“…This result showed the significant inhibition of cell proliferation with irradiation in CNE-2 cells. Furthermore, it is in agreement with several reports (Seitz et al, 2008;Mikes et al, 2009;Ferenc et al, 2010), showing HY had the ability to inhibit biological activity with irradiation.…”

Section: Discussionsupporting

confidence: 93%

Cellular and Molecular Mechanisms of Photodynamic Hypericin Therapy for Nasopharyngeal Carcinoma Cells

Wang

Guo²,

Yang³

et al. 2010

J Pharmacol Exp Ther

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Hypericin-mediated photodynamic therapy (HY-PDT) has become a potential treatment for tumors and nonmalignant disorders. Some studies reported that HY-PDT could lead to apoptosis in some carcinoma cells. However, the molecular mechanism of HY-PDT remains unknown. In this study, we evaluated the molecular mechanisms of hypericin associated with light-emitting diode irradiation on the poorly differentiated human nasopharyngeal carcinoma cell line CNE-2 in vitro. To comprehensively understand the effects of HY-PDT on CNE-2 cells, we detected cell viability, cell cycle, apoptosis, intracellular glutathione content, and intracellular caspase (caspase-9, caspase-3, and caspase-8) activity. Furthermore, we performed genome-wide expression analysis via microarrays at different time points in response to HY-PDT, and we found that differentially expressed genes were highly enriched in the pathways related to reactive oxygen species generation, mitochondrial activity, DNA replication and repair, cell cycle/proliferation, and apoptosis. These results were consistent with our cytology test results and demonstrated that caspasedependent apoptosis occurred after HY-PDT. Taken together, both cellular and molecular data revealed that HY-PDT could inhibit the growth of CNE-2 cells and induce their apoptosis.

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Section: Discussionsupporting

confidence: 93%

Cellular and Molecular Mechanisms of Photodynamic Hypericin Therapy for Nasopharyngeal Carcinoma Cells

Wang

Guo²,

Yang³

et al. 2010

J Pharmacol Exp Ther

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“…In contrast, the overexpression of survivin increased cell viability and reduced cell death. Expression of another antiapoptotic protein, Bcl-2, was suppressed by genistein, and PDT with hypericin may represent a mutual therapeutic combination favoring apoptosis [34] . The combination of genistein and PDT may therefore achieve a higher therapeutic outcome in human breast adenocarcinoma cell lines previously identified as PDT-resistant.…”

Section: Pdt Combined With Anti-apoptotic Strategiesmentioning

confidence: 98%

Breast cancer as photodynamic therapy target: Enhanced therapeutic efficiency by overview of tumor complexity

Lamberti

Vittar

Rivarola

2014

WJCO

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Photodynamic therapy is a minimally invasive and clinically approved procedure for eliminating selected malignant cells with specific light activation of a photosensitizer agent. Whereas interstitial and intra-operative approaches have been investigated for the ablation of a broad range of superficial or bulky solid tumors such as breast cancer, the majority of approved photodynamic therapy protocols are for the treatment of superficial lesions of skin and luminal organs. This review article will discuss recent progress in research focused mainly on assessing the efficacies of various photosensitizers used in photodynamic therapy, as well as the combinatory strategies of various therapeutic modalities for improving treatments of parenchymal and/or stromal tissues of breast cancer solid tumors. Cytotoxic agents are used in cancer treatments for their effect on rapidly proliferating cancer cells. However, such therapeutics often lack specificity, which can lead to toxicity and undesirable side effects. Many approaches are designed to target tumors. Selective therapies can be established by focusing on distinctive intracellular (receptors, apoptotic pathways, multidrug resistance system, nitric oxidemediated stress) and environmental (glucose, pH) differences between tumor and healthy tissue. A rational design of effective combination regimens for breast cancer treatment involves a better understanding of the mechanisms and molecular interactions of cytotoxic agents that underlie drug resistance and sensitivity. Key words: Photodynamic therapy; Breast cancer; Tumor microenvironment; Treatment combination; Synergism Core tip: Breast cancer is the most common cancer in women worldwide. However, effective therapies that reduce the high mortality rate and improve patient quality of life are still unavailable. In recent years, the use of photodynamic therapy has been examined for use in breast cancer treatment. Photodynamic therapy provides a new and promising antitumor strategy that could be implemented, alone or in combination with other approved or experimental therapeutic approaches, to a wide range of applications.

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“…Cell line References Akt inhibition HT-29 Sačková et al, 2006 activation/ inhibition human dermal fibroblasts Schieke et al, 2004activation BA, BT-474, NIH 3T3, MCF-7 Bozkulak et al, 2007, Zhuang and Kochevar, 2003, Ferenc et al, 2010depletion SKBR-3 Solár et al, 2011 Erk …”

Section: Molecule Modificationsmentioning

confidence: 99%

“…Moreover, GE eliminated irradiation-induced activation of Akt and Erk1/2 (Akimoto et al, 2001). Application of GE or HY-PDT alone in the study by Ferenc et al (2010) demonstrated both types of reaction; stimulated Akt and Erk1/2 phosphorylation in MCF-7 cells as well as no effect (Erk1/2; PDT) or even dephosphorylation in MDA-MB-231 cells. Moreover pretreatment with GE prior to PDT led to suppression of phosphorylation status of Akt and Erk1/2 in both cell lines.…”

mentioning

confidence: 90%

“…Furthermore, Akt protein levels depleted after HY-PDT with GE pre-treatment did not correlated well with mRNA level, which was unaffected. Theoretically, post-translation modification of Akt and Erk1/2 could be partly responsible for effective reduction of proliferarion and clonogenic ability as well as induction of apoptosis recorded in breast adenocarcinoma cells (Ferenc et al, 2010). One interesting fact revealed in another study (Solár et al, 2011) was a drop in Akt and Erk1/2 activity after elevated oxidative stress achieved by high dose of HY-PDT.…”

mentioning

confidence: 92%

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Breast Cancer and Current Therapeutic Approaches: From Radiation to Photodynamic Therapy

Ferenc¹,

Solár²,

Mikeš³

et al. 2011

Breast Cancer - Current and Alternative Therapeutic Modalities

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Down-regulation of Bcl-2 and Akt induced by combination of photoactivated hypericin and genistein in human breast cancer cells

Cited by 61 publications

References 51 publications

Cellular and Molecular Mechanisms of Photodynamic Hypericin Therapy for Nasopharyngeal Carcinoma Cells

Cellular and Molecular Mechanisms of Photodynamic Hypericin Therapy for Nasopharyngeal Carcinoma Cells

Breast cancer as photodynamic therapy target: Enhanced therapeutic efficiency by overview of tumor complexity

Breast Cancer and Current Therapeutic Approaches: From Radiation to Photodynamic Therapy

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