Down-regulation of Bcl2 and Survivin, and up-regulation of Bax involved in copper (II) phenylthiosemicarbazone complex-induced apoptosis in leukemia stem-like KG1a cells

Haddadi, Parinaz; Mahdavi, Majid; Rahbarghazi‬, Reza

doi:10.1016/j.procbio.2020.01.010

Cited by 4 publications

(1 citation statement)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Subsequently, 0.1 μg of RNA from each sample was subjected to reverse transcription using the HyperScript™ First-strand synthesis kit (GeneAll, Seoul, Republic of Korea). A real-time polymerase chain reaction (RT-PCR) was performed with a Rotor-Gene Q (Qiagen) using RealQ Plus 2× Master Mix Green (Ampliqon, Odense, Denmark) [ 35 ]. The thermal cycling conditions involved an initial activation step for 15 min at 95 °C followed by 40 cycles, including a denaturation step for 20 s at 95 °C and a combined annealing/extension step for 60 s at 60 °C.…”

Section: Methodsmentioning

confidence: 99%

The Combined Effect of Licorice Extract and Bone Marrow Mesenchymal Stem Cells on Cisplatin-Induced Hepatocellular Damage in Rats

et al. 2023

View full text Add to dashboard Cite

Drug-induced liver damage is a life-threatening disorder, and one major form of it is the hepatotoxicity induced by the drug cisplatin. In folk medicine, Licorice (Glycyrrhiza glabra (is used for detoxification and is believed to be a potent antioxidant. Currently, the magically self-renewable potential of bone marrow mesenchymal stem cells (BM-MSCs) has prompted us to explore their hepatoregenerative capability. The impact of G. glabra extract (GGE) and BM-MSCs alone and, in combination, on protecting against hepatotoxicity was tested on cisplatin-induced liver injury in rats. Hepatic damage, as revealed by liver histopathology and increased levels of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and malondialdehyde (MDA), was elevated in rats by received 7 mg/kg of cisplatin intraperitoneally. The combination of GGE and BM-MSCs returned the enzyme levels to near the normal range. It also improved levels of liver superoxide dismutase (SOD) and glutathione (GSH) and reduced MDA levels. Additionally, it was found that when GGE and BM-MSCs were used together, they significantly downregulated caspase9 (Casp9), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB), and interleukin-1β (IL-1β), which are involved in severe proinflammatory and apoptotic signaling cascades in the liver. Moreover, combining GGE and BM-MSCs led to the normal result of hepatocytes in several examined liver histological sections. Therefore, our findings suggest that GGE may have protective effects against oxidative liver damage and the promising regenerative potential of BM-MSCs.

show abstract

Section: Methodsmentioning

confidence: 99%