Down‐regulation of BnDA1, whose gene locus is associated with the seeds weight, improves the seeds weight and organ size in <i>Brassica napus</i>

Jieli, Wang; Tang, Minghua; Chen, Sheng; Zheng, Xizhong; Mo, Huixian; Li, Shengjun; Wang, Zheng; Zhu, Kehua; Ding, Lei; S, Liu; Li, Yunhai; Tan, Xiao‐Li

doi:10.1111/pbi.12696

Cited by 64 publications

(59 citation statements)

References 45 publications

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“…Previous studies of DA1 focused on the AtDA1 R358K mutation in the da1‐1 dominant‐negative mutant. This mutation inhibited the activities of DA1 and DAR1, significantly increasing seed sizes in Arabidopsis , as well as transgenic rapeseed and maize (Li et al , ; Wang et al , ; Xie et al , ); however, this mutation has not been identified in natural variations so far. In this study, we sequenced many natural variations in TaDA1‐A , most of which were located in the promoter and intronic regions (Figure a).…”

Section: Discussionmentioning

confidence: 99%

TaDA1, a conserved negative regulator of kernel size, has an additive effect with TaGW2 in common wheat (Triticum aestivum L.)

Hao

et al. 2019

Plant Biotechnology Journal

104

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Summary Kernel size is an important trait determining cereal yields. In this study, we cloned and characterized TaDA1, a conserved negative regulator of kernel size in wheat (Triticum aestivum). The overexpression of TaDA1 decreased the size and weight of wheat kernels, while its down‐regulation using RNA interference (RNAi) had the opposite effect. Three TaDA1‐A haplotypes were identified in Chinese wheat core collections, and a haplotype association analysis showed that TaDA1‐A‐HapI was significantly correlated with the production of larger kernels and higher kernel weights in modern Chinese cultivars. The haplotype effect resulted from a difference in TaDA1‐A expression levels between genotypes, with TaDA1‐A‐HapI resulting in lower TaDA1‐A expression levels. This favourable haplotype was found having been positively selected during wheat breeding over the last century. Furthermore, we demonstrated that TaDA1‐A physically interacts with TaGW2‐B. The additive effects of TaDA1‐A and TaGW2‐B on kernel weight were confirmed not only by the phenotypic enhancement arising from the simultaneous down‐regulation of TaDA1 and TaGW2 expression, but also by the combinational haplotype effects estimated from multi‐environment field data from 348 wheat cultivars. A comparative proteome analysis of developing transgenic and wild‐type grains indicated that TaDA1 and TaGW2 are involved in partially overlapping but relatively independent protein regulatory networks. Thus, we have identified an important gene controlling kernel size in wheat and determined its interaction with other genes regulating kernel weight, which could have beneficial applications in wheat breeding.

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Section: Discussionmentioning

confidence: 99%

TaDA1, a conserved negative regulator of kernel size, has an additive effect with TaGW2 in common wheat (Triticum aestivum L.)

Hao

et al. 2019

Plant Biotechnology Journal

104

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“…Recent studies have shown that ubiquitin-proteasome pathways are involved in control of seed size (Li and Li, 2016 ; Nadolska-Orczyk et al , 2017 ; Kelley, 2018 ; Li et al , 2019 b ; Xu and Xue, 2019 ), and one of these, the DA1 pathway, plays a key role in controlling seed size in Arabidopsis by regulating cell proliferation in the integuments ( Disch et al , 2006 ; Xia et al , 2013 a ; Du et al , 2014 ; Li and Li, 2016 ; Dong et al , 2017 ). This pathway of control of seed size is highly conserved among species ( Su et al , 2011 ; Wang et al , 2017 ; Xie et al , 2018 ). For example, Grain Width and Weight2 ( GW2 ), a major quantitative trait locus (QTL) in rice, is a homolog of DA2 , a RING-type E3 ubiquitin ligase gene ( Song et al , 2007 ; Xia et al , 2013 a ).…”

Section: Introductionmentioning

confidence: 99%

RING finger ubiquitin E3 ligase gene TaSDIR1-4A contributes to determination of grain size in common wheat

Wang

Mao

et al. 2020

Journal of Experimental Botany

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Salt and Drought Induced RING finger1 (SDIR1) is a RING-type E3 ubiquitin ligase that plays a pivotal role in ABA-mediated response to salinity and drought stress by the ubiquitination pathway in some plant species. However, its function in wheat (Triticum aestivum) is unknown. Here, we isolated a SDIR1 member, TaSDIR1-4A in wheat and characterized its E3 ubiquitin ligase activity. DNA polymorphism assays showed the presence of two nucleotide variation sites in the promoter region of TaSDIR1-4A; these formed haplotypes Hap-4A-1 and Hap-4A-2 that were detected in wheat populations. Association analysis showed that TaSDIR1-4A was associated with 1,000-grain weight (TGW) in all test environments, including well watered and heat stress situations. Genotypes with Hap-4A-2 had higher TGW than those with Hap-4A-1. The phenotypes in both gene-silenced wheat and transgenic Arabidopsis showed that TaSDIR1-4A was a negative regulator of grain size. Gene expression assays revealed that TaSDIR1-4A was most highly expressed in flag leaves, and expression levels of TaSDIR1-4A were higher in Hap-4A-1 accessions than in Hap-4A-2 accessions. The difference might be attributable to TaERF3 (Ethylene Response Factor) can act as a transcriptional repressor of TaSDIR1-4A in Hap-4A-2, but not of TaSDIR1-4A in Hap-4A-1. The favorable haplotype has been positively selected in breeding programs in China. The functional marker for TaSDIR1-4A should be helpful for wheat breeding.

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“…Many research groups worldwide have obtained transgenic or mutant plants exhibiting higher yields than controls (Zhao et al, 2015;Cabello et al, 2017;Wang et al, 2017;Jiang et al, 2018;Lim et al, 2018). However, the evaluation of stems width and the number of vascular bundles in these plants was not investigated or associated with seed yield.…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis and sunflower plants with increased xylem area show enhanced seed yield

Cabello

Chan

2019

The Plant Journal

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Summary Plant architecture plasticity determines the efficiency at harvesting and plays a major role defining biomass and seed yield. We observed that several previously described transgenic genotypes exhibiting increased seed yield also show wider stems and more vascular bundles than wild‐type plants. Here, the relationship between these characteristics and seed yield was investigated. Hanging weight on the main stem of Arabidopsis plants provoked significant stem widening. Such widening was accompanied by an increase in the number of vascular bundles and about 100% of yield increase. In parallel, lignin deposition diminished. Vascular bundle formation started in the upper internode and continued downstream. AUX/LAX carriers were essential for this response. The increase of vascular bundles was reverted 3 weeks after the treatment leading to an enlarged xylem area. Aux1, lax1, and lax3 mutant plants were also able to enlarge their stems after the treatment, whereas lax2 plants did not. However, none of these mutants exhibited more vascular bundles or seed yield compared with untreated plants. Weight‐induced xylem area enhancement and increased seed yield were also observed in sunflower plants. Altogether these results showed a strong correlation between the number of vascular bundles and enhanced seed yield under a long‐day photoperiod. Furthermore, changes in the levels of auxin carriers affected both these processes in the same manner, suggesting that there may be an underlying causality.

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Down‐regulation of BnDA1, whose gene locus is associated with the seeds weight, improves the seeds weight and organ size in Brassica napus

Cited by 64 publications

References 45 publications

TaDA1, a conserved negative regulator of kernel size, has an additive effect with TaGW2 in common wheat (Triticum aestivum L.)

TaDA1, a conserved negative regulator of kernel size, has an additive effect with TaGW2 in common wheat (Triticum aestivum L.)

RING finger ubiquitin E3 ligase gene TaSDIR1-4A contributes to determination of grain size in common wheat

Arabidopsis and sunflower plants with increased xylem area show enhanced seed yield

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