Down-regulation of defender against apoptotic death (DAD1) after yellow head virus (YHV) challenge in black tiger shrimp Penaeus monodon

Molthathong, Sudkhate; Senapin, Saengchan; Klinbunga, Sirawut; Puanglarp, Narongsak; Rojtinnakorn, Jiraporn; Flegel, Timothy W.

doi:10.1016/j.fsi.2007.10.013

Cited by 21 publications

(14 citation statements)

References 26 publications

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“…Other studies have reported that expression of non-specific nucleases occurs in the digestive gland of Penaeus japonicus and tiger shrimp Penaeus monodon (29), where zymographic analysis shows that they degrade as much DNA as RNA, according with our results.The results of the digestibility of dsRNA-ORF89 in the digestive tract of the shrimp, reduce the possibilities of its inclusion of naked form (without protection).This information is important to design an adequate vehicle to incorporate specific dsRNA, so that it retains its antiviral property when passing through the digestive tract. Potential vehicles for the delivery of dsRNA, to reduce their hydrolysis in the digestive tract of shrimp, include nanocarriers based on cationic liposomes (nano and micro encapsulation systems), which because of their amphipathic nature are more permeable in the Phospholipid membrane of the cells, favoring the entrance of the specific dsRNA (30,9).…”

Section: Discussionsupporting

confidence: 90%

“…minuto -1 ). En otros trabajos se han reportado nucleasas no específicas en la glándula digestiva del camarón japonés Penaeus japonicus y el camarón tigre Penaeus monodon (29), donde el análisis zimográfico muestra que las nucleasas inespecíficas de la glándula digestiva degradan tanto ADN como ARN, en concordancia con nuestros resultados. Los resultados de la digestibilidad de dsRNA-ORF89 en el tracto digestivo del camarón, reducen las posibilidades de su inclusión de forma desnuda (sin protección).…”

Section: Discussionunclassified

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Digestibilidad in vitro del dsRNA específico por enzimas del tracto digestivo del camarón Litopenaeus vannamei

2017

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Objective. The digestibility of specific dsRNA by action of the enzymes of digestive tract of the whiteleg shrimp Litopenaeus vannamei was determined in vitro. Materials and methods. Digestive enzyme activity (amylase, lipase, protease, DNase and RNase) was measured in the stomach, digestive gland, and anterior, middle, and posterior intestine of juvenile shrimp, and the digestibility of DNA, RNA and the dsRNA-ORF89, specific to WSSV, was determined by in vitro assays, as well as electrophoretic and densitometric analyses. Results. The highest enzymatic activity was found in the digestive gland: amylase (81.41%), lipase (92.60%), protease (78.20%), DNase (90.85%), and RNase (93.14%). The highest digestive capacity against DNA, RNA, and dsRNA was found in the digestive gland (5.11 ng of DNA per minute, 8.55 ng of RNA per minute, and 1.48 ng dsRNA per minute). Conclusions. The highest digestibility of dsRNA-ORF89, specific to WSSV, was found in the digestive gland, whereas the lowest digestibility was observed in the posterior intestine. This is the first report regarding the digestibility of dsRNA-ORF89 by whiteleg shrimp digestive tract enzymes, with potential therapeutic importance in shrimp culture to prevent WSSV disease through balanced feed.Keywords: Aquaculture, balanced feed, digestive gland, nucleic acids, WSSV (Sources: MeSH, FAO). RESUMENObjetivo. La digestibilidad del dsRNA específico para el virus de la mancha blanca (WSSV) por acción de las enzimas del tracto digestivo del camarón Litopenaeus vannamei fue analizada in vitro. Material y métodos. Se midió la actividad de enzimas digestivas (proteasa, amilasa, lipasa, ADNasa y ARNasa) en el estómago, la glándula digestiva, el intestino anterior, medio y posterior en juveniles de camarón patiblanco y se evaluó la digestibilidad de ácidos nucleicos ADN, ARN y dsRNA-ORF89 especifico contra el virus WSSV, por análisis electroforéticos y densitometría. Resultados. La actividad enzimática más alta se encontró en la glándula digestiva del camarón: amilasa (81.41%), lipasa (92.60%), proteasa (78.20%), ADNasa (90.85%) y ARNasa (93.14%). Se evidenció la capacidad digestiva del camarón patiblanco contra el ADN, ARN y dsRNA-ORF89 encontrando en la glándula digestiva la mayor digestión (5.11 ng de ADN por minuto, 8.55 ng de ARN por minuto y 1.48 ng de dsRNA por minuto). Conclusiones. La mayor digestibilidad del dsRNA-ORF89, específico contra el virus WSSV, se encontró en la glándula digestiva y la menor en el intestino posterior. Este es el primer informe relacionado con la digestibilidad del dsRNA-ORF89 por las enzimas del camarón patiblanco con potencial importancia terapéutica en el cultivo de camarón para prevenir la enfermedad del WSSV a través del alimento balanceado. INTRODUCCIÓNUna de las herramientas más poderosas utilizadas en la acuicultura frente a patógenos virales como el virus de la mancha blanca (WSSV) es el mecanismo de ARN de interferencia (ARNi) inducido por ARN de doble cadena (dsRNA, por sus siglas en ingles double-stranded RNA) (1). P...

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Section: Discussionsupporting

confidence: 90%

Section: Discussionunclassified

Digestibilidad in vitro del dsRNA específico por enzimas del tracto digestivo del camarón Litopenaeus vannamei

2017

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“…The programs and protocols used for sequence alignment and phylogenetic tree construction are described in a companion manuscript [14]. N-linked glycosylation sites were predicted according to …”

Section: Sequence Analysis and Phylogenetic Tree Analysismentioning

confidence: 99%

“…Methods for determining tissue distribution (including shrimp specimen source and handling, tissues used, RNA extraction and real-time RTePCR) are described in the companion manuscript [14]. Additional information specific to ribophorin 1 analysis follows.…”

Section: Tissue Distribution Of P Monodon Ribophorin Imentioning

confidence: 99%

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Up-regulation of Ribophorin I after yellow head virus (YHV) challenge in black tiger shrimp Penaeus monodon

Molthathong

Buaklin

Senapin

et al. 2008

Fish & Shellfish Immunology

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Immunology of Invertebrates: Cellular

Smith¹

2010

Encyclopedia of Life Sciences

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Circulating (free) cells are the main mediators of immunity in invertebrates, carrying out the phagocytic, encapsulating and microbicidal responses that enable these animals to protect themselves against infection without a specific adaptive immune system. The cells are specialised for these functions, with division of labour between types. In general, immune cell diversity tends to increase with body complexity and life histories but common to the majority of species, however, are phagocytic and granular cell types that synthesise and store bioactive proteins. The release of these compounds occurs by exocytosis following interaction of pattern recognition receptors and nonself motifs carried on the surface of pathogens or parasites. Cell death of one form or another appears to be inherent in immune reactivity of many invertebrates with new cells produced, at least in coelomates, by mesodermal haemopoietic tissues. Key concepts Immunity in invertebrates is confined to nonspecific inflammatory responses, mediated to a large extent by the circulating blood cells (haemocytes or coelomocytes) or their products. All coelomate invertebrates contain populations of freely circulating cells dedicated to host defence, some well developed for specialist purposes. There are no true vertebrate‐type lymphocytes and no long‐term, highly specific immune memory in invertebrates. The main invertebrate cellular immune responses include phagocytosis, encapsulation, cytotoxicity and the synthesis and release of microbicidal agents. These cellular reactions often involve the death of immune cells or their removal from the circulation, requiring new cells to be synthesised in the haemopoietic tissue and released into the blood. Genomic and proteomic studies have enabled many of the proteins responsible for cellular defence in invertebrates to be identified, especially in arthropods. Although many of the responses and pathways are ancient and highly conserved, there is great diversity in the effector molecules across the invertebrates as a whole.

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Down-regulation of defender against apoptotic death (DAD1) after yellow head virus (YHV) challenge in black tiger shrimp Penaeus monodon

Cited by 21 publications

References 26 publications

Digestibilidad in vitro del dsRNA específico por enzimas del tracto digestivo del camarón Litopenaeus vannamei

Digestibilidad in vitro del dsRNA específico por enzimas del tracto digestivo del camarón Litopenaeus vannamei

Up-regulation of Ribophorin I after yellow head virus (YHV) challenge in black tiger shrimp Penaeus monodon

Immunology of Invertebrates: Cellular

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