Down‐regulation of <i><scp>CBP</scp>80</i> gene expression as a strategy to engineer a drought‐tolerant potato

Pieczynski, Marcin; Marczewski, Waldemar; Hennig, Jacek; Dolata, Jakub; Bielewicz, Dawid; Piontek, Paulina; Wyrzykowska, Anna; Krusiewicz, Dominika; Strzelczyk-Żyta, Danuta; Konopka-Postupolska, Dorota; Krzesłowska, Magdalena; Jarmołowski, Artur; Szweykowska-Kulińska, Zofia

doi:10.1111/pbi.12032

Cited by 145 publications

(114 citation statements)

References 43 publications

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“…It should be noted that the guard cells of mutant were smaller, more precisely shorter (80% of the WT) and wider (124% of the WT). These results are in accordance with previously reported cbp80 mutants of A. thaliana and S. tuberosum , which exhibited fewer stomata (respectively, 75 and 80% of the WT) and more trichomes (respectively, 140% and 130% of the WT) on the adaxial side of the leaves (Daszkowska-Golec et al, 2013; Pieczynski et al, 2013). …”

Section: Resultssupporting

confidence: 92%

“…Interestingly, the Arabidopsis knockout mutant in CBP80 ( Cap-Binding Protein 80; ABA hypersensitive 1 ) exhibited a similar phenotype when exposed to ABA or drought stress (Hugouvieux et al, 2001, 2002; Daszkowska-Golec et al, 2013). In Solanum tuberosum , an amiR80.2-14 mutant ( CBP80 silenced using artificial microRNAs) was also reported to be drought tolerant (Pieczynski et al, 2013). …”

Section: Introductionmentioning

confidence: 99%

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Mutation in HvCBP20 (Cap Binding Protein 20) Adapts Barley to Drought Stress at Phenotypic and Transcriptomic Levels

et al. 2017

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CBP20 (Cap-Binding Protein 20) encodes a small subunit of the cap-binding complex (CBC), which is involved in the conserved cell processes related to RNA metabolism in plants and, simultaneously, engaged in the signaling network of drought response, which is dependent on ABA. Here, we report the enhanced tolerance to drought stress of barley mutant in the HvCBP20 gene manifested at the morphological, physiological, and transcriptomic levels. Physiological analyses revealed differences between the hvcbp20.ab mutant and its WT in response to a water deficiency. The mutant exhibited a higher relative water content (RWC), a lower stomatal conductance and changed epidermal pattern compared to the WT after drought stress. Transcriptome analysis using the Agilent Barley Microarray integrated with observed phenotypic traits allowed to conclude that the hvcbp20.ab mutant exhibited better fitness to stress conditions by its much more efficient and earlier activation of stress-preventing mechanisms. The network hubs involved in the adjustment of hvcbp20.ab mutant to the drought conditions were proposed. These results enabled to make a significant progress in understanding the role of CBP20 in the drought stress response.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Mutation in HvCBP20 (Cap Binding Protein 20) Adapts Barley to Drought Stress at Phenotypic and Transcriptomic Levels

et al. 2017

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“…In the model plant Arabidopsis, an increased amount of cuticular material of the ABA oversensitive cbp20 mutant has been associated with improved drought tolerance . In potato lines silenced for an ortholog of the Arabidopsis ABH1/CBP80 gene, more compact cuticular ultrastructure was observed, correlating with a higher rate of water retention of leaves (Pieczynski et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

A morpho-physiological approach differentiates bread wheat cultivars of contrasting tolerance under cyclic water stress

Jäger

Fábián

Eitel

et al. 2014

Journal of Plant Physiology

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Leaf micromorphological traits and some physiological parameters with potential relevance to drought tolerance mechanisms were investigated in four selected winter wheat varieties. Plants were subjected to two cycles of drought treatment at anthesis. Yield components confirmed contrasting drought-sensitive and -tolerant behavior of the genotypes. Drought tolerance was associated with small flag leaf surfaces and less frequent occurrence of stomata. Substantial variation of leaf cuticular thickness was found among the cultivars. Thin cuticle coincided with drought sensitivity and correlated with a high rate of darkadapted water loss from leaves. Unlike in Arabidopsis, thickening of the cuticular matrix in response to water deprivation did not occur. Water stress induced epicuticular wax crystal depositions preferentially on the abaxial leaf surfaces. According to microscopy and electrolyte leakage measurements from leaf tissues, membrane integrity was lost earlier or to a higher extent in sensitive than in tolerant genotypes. Cellular damage and a decline of relative water content of leaves in sensitive cultivars became distinctive during the second cycle of water deprivation. Our results indicate strong variation of traits with potential contribution to the complex phenotype of drought tolerance in wheat genotypes. The maintained membrane integrity and relative water content values during repeated water limited periods were found to correlate with drought tolerance in the selection of cultivars investigated.

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“…We used a hollow metal cylinder to punch out round leaf discs (surface 0.86 cm 2 ) from sampled leaves. Samples were taken only on the areas between leaf veins [31]. …”

Section: Methodsmentioning

confidence: 99%

Plant Responses to Brief Touching: A Mechanism for Early Neighbour Detection?

et al. 2016

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In natural habitats plants can be exposed to brief and light contact with neighbouring plants. This mechanical stimulus may represent a cue that induces responses to nearby plants. However, little is known about the effect of touching on plant growth and interaction with insect herbivores. To simulate contact between plants, a soft brush was used to apply light and brief mechanical stimuli to terminal leaves of potato Solanum tuberosum L. The number of non-glandular trichomes on the leaf surface was counted on images made by light microscope while glandular trichomes and pavement cells were counted on images made under scanning electronic microscope. Volatile compounds were identified and quantified using coupled gas chromatography–mass spectrometry (GC-MS). Treated plants changed their pattern of biomass distribution; they had lower stem mass fraction and higher branch and leaf mass fraction than untouched plants. Size, weight and number of tubers were not significantly affected. Touching did not cause trichome damage nor change their total number on touched terminal leaves. However, on primary leaves the number of glandular trichomes and pavement cells was significantly increased. Touching altered the volatile emission of treated plants; they released higher quantities of the sesquiterpenes (E)-β-caryophyllene, germacrene D-4-ol and (E)-nerolidol, and lower quantities of the terpenes (E)-ocimene and linalool, indicating a systemic effect of the treatment. The odour of touched plants was significantly less preferred by the aphids Macrosiphum euphorbiae and Myzus persicae compared to odour of untouched plants. The results suggest that light contact may have a potential role in the detection of neighbouring plants and may affect plant-insect interactions.

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Down‐regulation of CBP80 gene expression as a strategy to engineer a drought‐tolerant potato

Cited by 145 publications

References 43 publications

Mutation in HvCBP20 (Cap Binding Protein 20) Adapts Barley to Drought Stress at Phenotypic and Transcriptomic Levels

Mutation in HvCBP20 (Cap Binding Protein 20) Adapts Barley to Drought Stress at Phenotypic and Transcriptomic Levels

A morpho-physiological approach differentiates bread wheat cultivars of contrasting tolerance under cyclic water stress

Plant Responses to Brief Touching: A Mechanism for Early Neighbour Detection?

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