Down-regulation of lycopene ε-cyclase expression in transgenic sweetpotato plants increases the carotenoid content and tolerance to abiotic stress

Ke, Qingbo; Kang, Le; Kim, Ho Soo; Xie, Tian; Liu, Chunjuan; Ji, Cheng; Kim, Sun Ha; Park, Woo Sung; Ahn, Mi‐Jeong; Wang, Shiwen; Li, Hongbing; Deng, Xin; Kwak, Sang‐Soo

doi:10.1016/j.plantsci.2019.01.002

Cited by 50 publications

(23 citation statements)

References 51 publications

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“…It starts with the condensation of two molecules of geranylgeranyl diphosphate (GGPP) by phytoene synthase ( PSY ) to generate phytoene, which normally is not accumulated in tissues. This step is a key rate-limiting stage of carotenoid biosynthesis, since it might affect the carotenoid pool content (Cazzonelli and Pogson, 2010; Ke et al, 2019). Following a sequence of desaturation and isomerization reactions catalyzed by phytoene desaturase ( PDS ), zeta-carotene isomerase ( Z-ISO ), zeta-carotene desaturase ( ZDS ), and carotenoid isomerase ( CRTISO ), the lycopene biosynthesis, a red-colored carotenoid, takes place.…”

Section: Carotenoid Biosynthesis and Degradation Pathwaysmentioning

confidence: 99%

Carotenoid Pigment Content in Durum Wheat (Triticum turgidum L. var durum): An Overview of Quantitative Trait Loci and Candidate Genes

et al. 2019

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Carotenoid pigment content is an important quality trait as it confers a natural bright yellow color to pasta preferred by consumers (whiteness vs. yellowness) and nutrients, such as provitamin A and antioxidants, essential for human diet. The main goal of the present review is to summarize the knowledge about the genetic regulation of the accumulation of pigment content in durum wheat grain and describe the genetic improvements obtained by using breeding approaches in the last two decades. Although carotenoid pigment content is a quantitative character regulated by various genes with additive effects, its high heritability has facilitated the durum breeding progress for this quality trait. Mapping research for yellow index and yellow pigment content has identified quantitative trait loci (QTL) on all wheat chromosomes. The major QTL, accounting for up to 60%, were mapped on 7L homoeologous chromosome arms, and they are explained by allelic variations of the phytoene synthase (PSY) genes. Minor QTL were detected on all chromosomes and associated to significant molecular markers, indicating the complexity of the trait. Despite there being currently a better knowledge of the mechanisms controlling carotenoid content and composition, there are gaps that require further investigation and bridging to better understand the genetic architecture of this important trait. The development and the utilization of molecular markers in marker-assisted selection (MAS) programs for improving grain quality have been reviewed and discussed.

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Section: Carotenoid Biosynthesis and Degradation Pathwaysmentioning

confidence: 99%

Carotenoid Pigment Content in Durum Wheat (Triticum turgidum L. var durum): An Overview of Quantitative Trait Loci and Candidate Genes

et al. 2019

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“…A study conducted by Kang et al (2018) showed overexpression of lycopene β-cyclase (IbLCYB2) significantly improved the contents of α-carotene (α-Car), β-Car, Lut, β-cryptoxanthin, and zeaxanthin (Zea) leading to enhancement of salt tolerance (Kang et al 2018). Ke et al (2019) also reported lycopene epsilon-cyclase (IbLCY-epsilon) gene downregulation in transgenic sweet potato can increase total carotenoid and β-Car contents, leading to the high activity of reactive oxygen species/radical scavenging, consequently, enhancing salt and drought tolerances (Ke et al 2019). Whereas, little concern about the relationship between the carotenoids and LL resistance is reported.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome sequence and physiological analysis revealed the roles of carotenoids and photosynthesis under low temperature combined with low-light stress on pepper (Capsicum annuum L.)

Li¹,

Xie²,

Yu³

et al. 2021

Photosynt.

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“…The expression of LCYβ increased to more than twice that of LCYε at 25 DAPs. Suppression of LCYε could enhance synthesis of β ‐carotene according to literature 39 . Therefore, the branch of zeaxanthin formation stayed high effeciency during maturation of BSC considered of high transcript level of LCYβ , which may be the reason causing abnormalhigh amount of zeaxanthin in BSC than other yellow sweet corns.…”

Section: Resultsmentioning

confidence: 88%

“…Suppression of LCYε could enhance synthesis of ⊎-carotene according to literature. 39 Therefore, the branch of zeaxanthin formation stayed high effeciency during maturation of BSC considered of high transcript level of LCY⊎, which may be the reason causing abnormalhigh amount of zeaxanthin in BSC than other yellow sweet corns. HYD and LUT5 together regulated the formation of zeinoxanthin, ⊎-cryptoxanthin and zeaxanthin where HYD and LUT5 both maintained high expression levels with some variation.…”

Section: Carotenoid and Vitamin E Content In Bsc During Kernel Develomentioning

confidence: 99%

Biosynthesis and accumulation of multi‐vitamins in black sweet corn (Zea mays L.) during kernel development

Liu

et al. 2020

J Sci Food Agric

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BACKGROUND: Black sweet corn as an edible fruit has various nutritional qualities. This study discusses changes in the vitamin C and E, folate, and carotenoid content during black sweet corn maturation, and also the effects of preharvest weather conditions and of related genes in multi-vitamin biosynthesis pathways. RESULTS: Most vitamin levels improved, especially vitamin C and carotenoid levels, while the folate content dropped rapidly. Transcript levels of most genes in folate biosynthesis showed trends that were similar to the content changes. VTC2 and GLDH, which are regulated by light, had high expression levels leading to an increase in ascorbate content during maturation. γ-Tocotrienol is the main vitamin E component, and HGGT, the key gene controlling the synthesis of tocotrienols, had a much higher expression level than other genes. Lutein and zeaxanthin were the dominant carotenoid components. A rapid reduction in the transcription level of LCYε could result in a lower lutein production rate. CONCLUSION: Black sweet corn has a high nutritional value and is rich in vitamins, including zeaxanthin, γ-tocotrienols, and ascorbic acid. The best harvest time is between 20-25 days after pollination (DAPs) when kernels had a good taste as well as relatively high vitamin levels.

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Down-regulation of lycopene ε-cyclase expression in transgenic sweetpotato plants increases the carotenoid content and tolerance to abiotic stress

Cited by 50 publications

References 51 publications

Carotenoid Pigment Content in Durum Wheat (Triticum turgidum L. var durum): An Overview of Quantitative Trait Loci and Candidate Genes

Carotenoid Pigment Content in Durum Wheat (Triticum turgidum L. var durum): An Overview of Quantitative Trait Loci and Candidate Genes

Transcriptome sequence and physiological analysis revealed the roles of carotenoids and photosynthesis under low temperature combined with low-light stress on pepper (Capsicum annuum L.)

Biosynthesis and accumulation of multi‐vitamins in black sweet corn (Zea mays L.) during kernel development

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