Down-Regulation of MiR-193a-3p Dictates Deterioration of HCC: A Clinical Real-Time qRT-PCR Study

Liu, Yongru; Ren, Fanghui; Luo, Yihuan; Rong, Minhua; Chen, Gang; Dang, Yi‐Wu

doi:10.12659/msm.894077

Cited by 24 publications

(26 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…**P < 0.01 compared with NC + SPOCK1-WT group. MiR-193a-3p dictated the deterioration of HCC-clinical analysis 10 and reduced the 5-FU resistance of HCC cells by mediating SRSF2. 23 Similarly, miR-193b also facilitated sorafenib and cisplatin induced apoptosis, 14,24 indicating that miR-193 could improve chemosensitivity.…”

Section: Discussionmentioning

confidence: 99%

MiR‐139‐5p, miR‐940 and miR‐193a‐5p inhibit the growth of hepatocellular carcinoma by targeting SPOCK1

Xiao

Luo

et al. 2019

J Cellular Molecular Medi

View full text Add to dashboard Cite

The study was aimed to screen out miRNAs with differential expression in hepatocellular carcinoma (HCC), and to explore the influence of the expressions of these miRNAs and their target gene on HCC cell proliferation, invasion and apoptosis. MiRNAs with differential expression in HCC were screened out by microarray analysis. The common target gene of these miRNAs (miR‐139‐5p, miR‐940 and miR‐193a‐5p) was screened out by analysing the target genes profile (acquired from Targetscan) of the three miRNAs. Expression levels of miRNAs and SPOCK1 were determined by quantitative real time polymerase chain reaction (qRT‐PCR). The target relationships were verified by dual luciferase reporter gene assay and RNA pull‐down assay. Through 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2‐H‐tetrazolium bromide,thiazolyl blue tetrazolium bromide (MTT) and transwell assays and flow cytometry, HCC cell viability, invasion and apoptosis were determined. In vivo experiment was conducted in nude mice to investigate the influence of three miRNAs on tumour growth. Down‐regulation of miR‐139‐5p, miR‐940 and miR‐193a‐5p was found in HCC. Overexpression of these miRNAs suppressed HCC cell viability and invasion, promoted apoptosis and inhibited tumour growth. SPOCK1 , the common target gene of miR‐139‐5p, miR‐940 and miR‐193a‐5p, was overexpressed in HCC. SPOCK1 overexpression promoted proliferation and invasion, and restrained apoptosis of HCC cells. MiR‐139‐5p, miR‐940 and miR‐193a‐5p inhibited HCC development through targeting SPOCK1 .

show abstract

Section: Discussionmentioning

confidence: 99%

MiR‐139‐5p, miR‐940 and miR‐193a‐5p inhibit the growth of hepatocellular carcinoma by targeting SPOCK1

Xiao

Luo

et al. 2019

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…16 For one thing, miR-193a-3p was significantly down-regulated within HCC tissues in comparison to the corresponding peri-tumoral tissues, suggesting that this it might be tumor-suppressive for HCC. 17 For another, higher miR-193a-3p levels were found within 5-FU-resistant HCC cell lines than the 5-FU-sensitive ones, and the miR-193a-3p/ SRSF2 (serine/arginine-rich splicing factor 2) axis could well be conducive to pre-determining the HCC subjects' response to 5-FU therapy. 18 Nevertheless, whether lncRNA H19 interacted with miR-193a-3p to modify the radio-resistance of HCC remained unclear.…”

mentioning

confidence: 99%

The LncRNA H19/miR‐193a‐3p axis modifies the radio‐resistance and chemotherapeutic tolerance of hepatocellular carcinoma cells by targeting PSEN1

Liu

et al. 2018

J of Cellular Biochemistry

View full text Add to dashboard Cite

This study was designated to verify if the lncRNA H19/miR-193a-3p axis would play a regulatory role in the radio-/chemo-resistances of HCC cells through targeting PSEN1. Within the study, five human HCC cell lines were prepared, including Bel-7402, HepG2, Hep3b, QGY-7703, and SMMC-7721. Moreover, docetaxel (DT), paclitaxel (Pt), vinorelbine (Vb), and 5-fluorouracil (5-Fu) were managed as the chemo-therapeutics, and single-dose X-rays were performed as radio-therapies. Besides, lncRNA H19 and miR-193a-3p were detected by qRT-PCR and Western blot were implemented to quantify the expressional levels of PSEN1, Ku80, γ-H2AX, and RAD51. Luciferase reporter gene assay was advanced to verify the targeted relationship between lncRNA H19 and miR-193a-3p. As a consequence, QGY-7703 and Bel-7402 were, respectively, the most radiation-sensitive and radiation-proof cell lines, and Bel-7402 was associated with the highest resistances to DT, Pt, Vb, and 5-FU. The restrained lncRNA H19 and over-expressed miR-193a-3p expressions tended to significantly elevate the survival rate and proliferation of Bel-7402 cells, when they were exposed to radiation and subject to chemo-therapies. The lncRNA H19 was also found to directly target miR-193a-3p in inducing the HCC development. PSEN1 appeared to be subject to the modification of lncRNA H19 and miR-193a-3p in its acting on the survival rates and proliferative abilities of HCC cells. The lncRNA H19/miR-193a-3p/PSEN1 axis could be regarded as the treatment targets for HCC, so as to further improve the treatment efficacy of chemo- and radio-therapies for HCC.

show abstract

“…No specific blood miRNA has been verified as a biomarker in the clinic for the early screening of NSCLC. Only two studies have explored the diagnostic value of miR‐193a‐3p, in hepatocellular carcinoma and colorectal cancer . miR‐193a‐3p attracted our attention and in the present study we attempted to investigate the potential value of miR‐193a‐3p in early screening of NSCLC based on microarray databases, and to further explore its prospective relevant pathways via bioinformatics analysis.…”

Section: Discussionmentioning

confidence: 99%

The clinical value of miR‐193a‐3p in non‐small cell lung cancer and its potential molecular mechanism explored in silico using RNA‐sequencing and microarray data

et al. 2018

Self Cite

View full text Add to dashboard Cite

miR‐193a‐3p is a tumor‐related miRNA playing an essential role in tumorigenesis and progression of non‐small cell lung cancer (NSCLC). The objective of the present study was to investigate the relationship between miR‐193a‐3p expression and clinical value and to further explore the potential signaling of miR‐193a‐3p in the carcinogenesis of NSCLC. RNA‐sequencing and microarray data were collected from the databases GEO, ArrayExpress and The Cancer Genome Atlas (TCGA). Furthermore, in silico assessments were performed to analyze the prospective pathways and networks of the target genes of miR‐193a‐3p. In total, 453 cases of NSCLC patients and 476 normal controls were included in blood samples, while 920 cases of NSCLC patients and 406 normal controls were included in tissue samples. The pooled positive likelihood ratio, the pooled negative likelihood ratio and the pooled diagnostic odds ratio were calculated to reflect the diagnostic value of miR‐193a‐3p in blood and tissue samples. Moreover, the areas under the curve of the summary receiver operating characteristic curve of blood and tissue were 0.64 and 0.79, respectively. In addition, we found a lower level of miR‐193a in NSCLC tissues than in non‐cancerous controls based on TCGA. A gene ontology (GO) enrichment analysis demonstrated that miR‐193a‐3p could be related to key signaling pathways in NSCLC. Also, several vital pathways were illustrated by KEGG. Lower expression of miR‐193a‐3p in tissue samples of NSCLC may be associated with tumorigenesis and be a predictor of deterioration of NSCLC patients, and pathway analysis revealed crucial signaling pathways correlated with the incidence and progress of NSCLC.

show abstract

Down-Regulation of MiR-193a-3p Dictates Deterioration of HCC: A Clinical Real-Time qRT-PCR Study

Cited by 24 publications

References 34 publications

MiR‐139‐5p, miR‐940 and miR‐193a‐5p inhibit the growth of hepatocellular carcinoma by targeting SPOCK1

MiR‐139‐5p, miR‐940 and miR‐193a‐5p inhibit the growth of hepatocellular carcinoma by targeting SPOCK1

The LncRNA H19/miR‐193a‐3p axis modifies the radio‐resistance and chemotherapeutic tolerance of hepatocellular carcinoma cells by targeting PSEN1

The clinical value of miR‐193a‐3p in non‐small cell lung cancer and its potential molecular mechanism explored in silico using RNA‐sequencing and microarray data

Contact Info

Product

Resources

About