Down-regulation of miR-23b may contribute to activation of the TGF-β1/Smad3 signalling pathway during the termination stage of liver regeneration

Yuan, Bin; Dong, Ruiqi; Shi, Duo; Zhou, Yun-heng; Zhao, Ying; Miao, Mingyong; Jiao, Binghua

doi:10.1016/j.febslet.2011.02.031

Cited by 86 publications

(60 citation statements)

References 28 publications

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“…First, certain similar miRNA changes are shared after hepatitis B (HBV) or salmonella infection and in our model. As reported, miR-27b-5p 56 and miR-23b-3p 57,58 were upregulated after HBV infection, and miR-26b, 59 mir-532-5p, 60 and mir-27b 60 were upregulated after salmonella infection. These miRNAs show the same tendency of changes after Ab stimulation.…”

Section: Discussionmentioning

confidence: 52%

“…These viral proteins can trigger cancer-related genes and motivate several signaling pathways including genetic instability, inflammatory, and host immune responses. 56 These pathways, especially the proliferation 57,61-63 and apoptosis 58,63 pathways, were included in our model. Thirdly, as we made predictions with limited confirmed miRNA data, some possible pathways might be missing as an inevitable undesired result.…”

Section: Discussionmentioning

confidence: 99%

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MicroRNA Expression Patterns Involved in Amyloid Beta–Induced Retinal Degeneration

Huang

Sun

Wang

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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Citation: Huang P, Sun J, Wang F, et al. MicroRNA expression patterns involved in amyloid beta-induced retinal degeneration. Invest Ophthalmol Vis Sci. 2017;58:172658: -173558: . DOI: 10.1167 PURPOSE. Dry age-related macular degeneration (AMD) is characterized by the accumulation of drusen under Bruch's membrane, and amyloid beta (Ab) is speculated to be one of the key pathologic factors. While the detrimental effects of Ab on retinas have been widely explored, Ab-induced epigenetic regulatory changes have yet to be fully investigated. We therefore aimed to identify the microRNA (miRNA) expression profiles in an Ab-induced mouse model of retinal degeneration.METHODS. C57BL/6 mice were intravitreally injected with Ab 1-40 or PBS and the eye tissues were collected for hematoxylin and eosin (H&E) staining, apoptosis immunofluorescence staining, and miRNA profiling. After filtering, 10 miRNAs and their target genes were chosen for quantitative RT-PCR (qRT-PCR) confirmations. Pathway analyses were employed for further bioinformatic analyses.RESULTS. Hematoxylin and eosin-stained sections of retinal pigment epithelium (RPE)/neural retina tissue demonstrated degenerative alterations, and immunofluorescence testing revealed apoptosis within the retina after Ab treatments. MicroRNA profiling revealed 61 miRNAs that were differentially expressed between the model and the control group. Among these, 38 miRNAs were upregulated (fold change > 1.5, P < 0.05) and 23 miRNAs were downregulated (fold change < 0.667, P < 0.05). Five of the 10 selected miRNAs (miR-142, miR-216, miR-155, miR-223, and miR-433) as well as several key target genes (CFH, IGF-1R, c-MET, and ABCA1) were confirmed by qRT-PCR analyses.CONCLUSIONS. Our study is the first to profile the miRNA expression patterns and suggests that Ab accumulation could lead to relevant biochemical alternations such as complement activation, barrier impairment, apoptosis, and positive feedback of Ab production.

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Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 99%

MicroRNA Expression Patterns Involved in Amyloid Beta–Induced Retinal Degeneration

Huang

Sun

Wang

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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“…8 It has been shown that certain micro RNAs may be involved in the termination of liver regeneration. 9,10 In addition, the ablation of integrin-linked kinase leads to enhanced liver proliferation.…”

Section: Partial Hepatectomy As a Model For The Study Of Mechanisms Wmentioning

confidence: 99%

Elimination of Tumor Suppressor Proteins during Liver Carcinogenesis

Timchenko

Lewis²

2014

Cancer Stud Mol Med Open J

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Liver cancer is one of the most lethal cancers. Quiescent liver expresses up to 20 tumor suppressor proteins including Rb, p53, CCAAT-Enhancer-Binding Protein (C/EBP)α, Hepatocyte Nuclear Factor (HNF4)α and p16 and it is well protected from development of liver cancer. However, the negative control of liver proliferation by these factors and other tumor suppressor genes is eliminated in liver cancer. Studies of liver regeneration after surgery and injury have provided fundamental mechanisms on how liver neutralizes tumor suppressor proteins for the time of regeneration; however, studies of liver cancer in animal models and in human samples showed several additional pathways of this neutralization. One of these additional pathways includes activation of a small subunit of the proteasome, Gankyrin. Gankyrin is dramatically increased in human hepatocellular carcinoma (HCC) and in animal models of carcinogenesis. Once activated Gankyrin triggers degradation of main tumor suppressor proteins during development of liver cancer using slightly different mechanisms. Recent studies identified mechanisms which repress Gankyrin in quiescent livers and mechanisms of activation of Gankyrin in liver cancer. These mechanisms involve a communication between Farnesoid X Receptor (FXR) signaling and chromatin remodelling proteins mediated by members of C/EBP family. It has been recently shown that C/EBPα plays a critical role in this network and that the activation of C/EBPα in cirrhotic livers with HCC inhibits cancer progression. This C/EBPα-dependent inhibition of liver cancer involves activation of a majority of tumor suppressor genes and repression of tumor initiating pathways such as β-catenin and c-myc. These recent findings provide a background for FXR-based and C/EBPα-based approaches to treat liver cancer.

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“…When injured or undergoing a partial hepatectomy (PH), hepatocytes are rapidly activated, proliferate, and restore the lost liver tissues; these processes are called liver regeneration (LR) (Taub, 2004;Xu et al, 2004). In recent years, an increasing number of studies have shown that microRNAs (miRNAs) such as miR-127, miR-34a, and miR-23b, play an important role in liver growth, development, and regeneration (Chen et al, 2011;Yuan et al, 2011;Pan et al, 2012). miRNAs are a class of endogenous, small regulatory RNA molecules with a length of about 25 nucleotides (nt), and are found mostly in introns and intergenic regions.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-21 promotes proliferation of rat hepatocyte BRL-3A by targeting FASLG

Li¹,

Chan²,

Leung³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Rat liver regeneration (RLR) induced by partial hepatectomy involves cell proliferation regulated by numerous factors, including microRNAs (miRNAs). miRNA high-throughput sequencing has been established and used to analyze miRNA expression profiles. This study showed that 39 miRNAs were related to RLR through the analysis of miRNA high-throughput sequencing. Their role toward rat normal hepatocyte line BRL-3A was studied by gain-and lossof-function analyses, and one of them, microRNA-21 (miR-21), obviously upregulated and promoted BRL-3A cell proliferation. Using bioinformatics to search for miR-21 targets revealed that Fas ligand (FASLG) is one of miR-21's target genes. A dual-luciferase report assay and Western blot assay showed that miR-21 directly targeted the 3'-untranslated region of FASLG and inhibited the expression of FASLG, which suggests that miR-21 promoted BRL-3A cell proliferation by reducing FASLG expression.

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Down-regulation of miR-23b may contribute to activation of the TGF-β1/Smad3 signalling pathway during the termination stage of liver regeneration

Cited by 86 publications

References 28 publications

MicroRNA Expression Patterns Involved in Amyloid Beta–Induced Retinal Degeneration

MicroRNA Expression Patterns Involved in Amyloid Beta–Induced Retinal Degeneration

Elimination of Tumor Suppressor Proteins during Liver Carcinogenesis

MicroRNA-21 promotes proliferation of rat hepatocyte BRL-3A by targeting FASLG

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