Down‐regulation of miRNA 145 and up‐regulation of miRNA 4516 may be associated with primary hypertension

Özkan, Gülsüm; Ulusoy, Şükrü; Geyik, Elif; Erdem, Yunus

doi:10.1111/jch.13704

Cited by 15 publications

(5 citation statements)

References 26 publications

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“…Furthermore, the significant relationship between miR-30a-5p expression and screen-detected and known HPT was demonstrated. Several studies have reported on a number of dysregulated miRNAs in HPT using different tissues, but results remain inconsistent (21)(22)(23)(24)(25). A study similar to ours reported 27 dysregulated miRNAs in a sample of 13 individuals with HPT (21), although the miRNAs were not similar to ours.…”

Section: Discussioncontrasting

confidence: 60%

MicroRNA Profiles in Normotensive and Hypertensive South African Individuals

Matshazi

Weale

Erasmus

et al. 2021

Front. Cardiovasc. Med.

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Hypertension has a complex pathogenesis and symptoms appear in advanced disease. Dysregulation of gene expression regulatory factors like microRNAs has been reported in disease development. Identifying biomarkers which could help understand the pathogenesis and prognosis of hypertension is essential. The study's objective was to investigate microRNA expression profiles according to participant blood pressure status. Next generation sequencing was used to identify microRNAs in the whole blood of 48 body mass index-, smoking- and age-matched normotensive (n = 12), screen-detected hypertensive (n = 16) and known hypertensive (n = 20) female participants. Quantitative reverse transcription polymerase chain reaction was used to validate the next generation sequencing findings in a larger, independent sample of 84 men and 179 women. Using next generation sequencing, 30 dysregulated microRNAs were identified and miR-1299 and miR-30a-5p were the most significantly differentially expressed. Both microRNAs were upregulated in known hypertensives or screen-detected hypertensives compared to the normotensives. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated possible involvement of platelet activation, calcium signaling and aldosterone synthesis pathways. Further validation of miR-1299 and miR-30a-5p using quantitative reverse transcription polymerase chain reaction confirmed sequencing results while yielding new findings. These findings demonstrate microRNA dysregulation in hypertension and their expression may be related to genes and biological pathways essential for blood pressure homeostasis.

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Section: Discussioncontrasting

confidence: 60%

MicroRNA Profiles in Normotensive and Hypertensive South African Individuals

Matshazi

Weale

Erasmus

et al. 2021

Front. Cardiovasc. Med.

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“…Thousands of miRNAs have been identified in humans, animals, and plants. Given their ubiquity, miRNAs have been extensively studied and proven to contribute to pathological conditions, such as hypertension (Biancardi & Sharma, 2020; Li et al, 2018; Ozkan et al, 2019). However, research on the relationship between miRNAs and the neurogenic pathogenesis of hypertension is limited.…”

Section: Discussionmentioning

confidence: 99%

“…An increasing number of reports have shown that miRNA contributes to many pathological and physiological processes (Ali Syeda et al, 2020; Cakmak & Demir, 2020; Juźwik et al, 2019; Bhaskaran & Mohan, 2014). Several miRNAs, such as miRNA‐21, miRNA‐145, miRNA‐4516, and miRNA‐181a, have been proven to be associated with hypertension (Biancardi & Sharma, 2020; Li et al, 2018; Ozkan et al, 2019). However, few researchers have looked into the miRNA profiles of the RVLM and their effects on sympathetic activation and blood pressure.…”

Section: Introductionmentioning

confidence: 99%

Up‐regulation of miR‐335 and miR‐674‐3p in the rostral ventrolateral medulla contributes to stress‐induced hypertension

Zhang

Xing

Chen

et al. 2022

Journal of Neurochemistry

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The rostral ventrolateral medulla (RVLM) is known as the vasomotor center that plays a crucial role in mediating the development of stress-induced hypertension (SIH).MicroRNAs (miRNAs) are involved in many different biological processes and diseases.However, studies that evaluated the roles of miRNAs in the RVLM during SIH do not exist. Here, we performed RNA sequencing to explore the genome-wide miRNA profiles in RVLM in an SIH rat model established by administering electric foot-shocks and noises. The function of miRNAs in blood pressure regulation was determined in vivo via the intra-RVLM microinjection of the agomir or antagomir. Furthermore, the underlying mechanisms of miRNAs on SIH were investigated through in vitro and in vivo experiments, like gain-of-function. We discovered 786 miRNA transcripts among which 4 were differentially expressed. The over-expression of miR-335 and miR-674-3p in RVLM dramatically increased the heart rate (HR), arterial blood pressure (ABP), systolic blood pressure (SBP), diastolic blood pressure (DBP), and mean arterial pressure (MAP) levels of normotensive rats, whereas the knockdown of miR-335 and miR-674-3p in RVLM markedly reduced the HR, ABP, SBP, DBP, and MAP levels of SIH rats. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation revealed that miR-335 and miR-674-3p participated in regulating the development of SIH from different aspects, like apoptosis-multiple species pathway. Sphk1, whose expression was markedly decreased in SIH, was identified as a novel target of miR-335. MiR-335 over-expression substantially reduced the expression of Sphk1 and promoted neural apoptosis, and its inhibition had opposite effects. Re-introduction of Sphk1 dramatically abrogated the apoptosis induced by miR-335. This study provides the first systematic dissection of the RVLM miRNA landscape in SIH. MiR-335 and miR-674-3p act as SIH promoters, and the identified miR-335/Sphk1/apoptosis axis represents one of the possible mechanisms. These miRNAs can be exploited as potential targets for the molecular-based therapy of SIH.

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“…RUNX1 [449], CAV1 [472], STAT6 [468], CDKN2A [226], HSPA2 [485], hsa-mir-10b-5p [617] and HOXA5 [618] were related to the obesity. RUNX1 [511], CAV1 [534], CACNA1A [543], STAT6 [528], CDKN2A [105], hsa-mir-4516 [619], SRY (sex determining region Y) [620], FOXC1 [621] and PRDM1 [622] might offer useful information for treating hypertension. CAV1 [303], CACNA1A [307], IRS4 [317] and hsa-mir-155-5p [623] expression have been found to be altered in patients with schizophrenia.…”

Section: Discussionmentioning

confidence: 99%

Screening of the Key Genes and Signaling Pathways for Schizophrenia Using Bioinformatics and Next Generation Sequencing Data Analysis

Vastrad,

Vastrad

2023

Preprint

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Schizophrenia is thought to be the most prevalent chronic psychiatric disorder. Numerous proteins have been identified that are associated with the occurrence and development of schizophrenia. This study aimed to identify potential core genes and pathways involved in schizophrenia, through exhaustive bioinformatic and next generation sequencing (NGS) data analyses using GSE20966 NGS data of neural progenitor cells and neurons obtained from healthy controls and patients with schizophrenia. The NGS data were downloaded from the Gene Expression Omnibus database. NGS data was processed by the DESeq2 package in R software and the differentially expressed genes (DEGs) were identified. Gene Ontology (GO) enrichment analysis and REACTOME pathway enrichment analysis were carried out to identify potential biological functions and pathways of the DEGs. Protein-protein interaction (PPI) network, module, miRNA-hub gene regulatory network and TF-hub gene regulatory network analysis were performed to identify the hub genes, miRNA and TFs. Potential hub genes were analyzed using receiver operating characteristic (ROC) curves in the R package (pROC). In this investigation, an overall 955 DEGs were identified: 478 genes were remarkably up regulated and 477 genes were distinctly down regulated. These genes were enriched for GO terms and pathways mainly involved in the multicellular organismal process, GPCR ligand binding, regulation of cellular process and amine ligand-binding receptors. MYC, FN1, CDKN2A, EEF1G, CAV1, ONECUT1, SYK, MAPK13, TFAP2A and BTK were considered the potential hub genes. miRNA-hub gene regulatory network and TF-hub gene regulatory network were constructed successfully. On the whole, the findings of this investigation enhance our understanding of the potential molecular mechanisms of schizophrenia and provide potential targets for further investigation.

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Down‐regulation of miRNA 145 and up‐regulation of miRNA 4516 may be associated with primary hypertension

Cited by 15 publications

References 26 publications

MicroRNA Profiles in Normotensive and Hypertensive South African Individuals

MicroRNA Profiles in Normotensive and Hypertensive South African Individuals

Up‐regulation of miR‐335 and miR‐674‐3p in the rostral ventrolateral medulla contributes to stress‐induced hypertension

Screening of the Key Genes and Signaling Pathways for Schizophrenia Using Bioinformatics and Next Generation Sequencing Data Analysis

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