Down-regulation of ribosomal protein L7A in human osteosarcoma

Zheng, Shuier; Yang, Yao; Dong, Yang; Lin, Feng; Zhao, Hui; Shen, Zan; Sun, Yong; Tang, Lina

doi:10.1007/s00432-008-0538-4

Cited by 25 publications

(13 citation statements)

References 31 publications

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“…In addition, it was reported that RPL7A expression was significantly down-regulated in OS tissues compared with that in normal bone tissues and benign bone lesions both in mRNA and protein levels. Low expression of RPL7A indicated a poor clinical outcome for overall survival in OS patients with lung metastasis at the time of initial diagnosis with primary OS41.…”

Section: Discussionmentioning

confidence: 99%

Highly expressed ribosomal protein L34 indicates poor prognosis in osteosarcoma and its knockdown suppresses osteosarcoma proliferation probably through translational control

Luo

Zhao

Fowdur

et al. 2016

Sci Rep

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Osteosarcoma has devastating health implications on children and adolescents. However, due to its low incidence and high tumor heterogeneity, it is hard to achieve any further improvements in therapy and overall survival. Ribosomal protein L34 (RPL34) has been increasingly recognized to promote the proliferation of malignant cells, but its role in osteosarcoma has not been investigated. In this study, real-time quantitative PCR (RT-qPCR) and immunohistochemistry revealed that RPL34 was highly expressed in osteosarcoma tissues when compared to adjacent tissues and normal bone tissues. Survival analysis showed that high expression of RPL34 predicted a poor prognosis for osteosarcoma patients. Knockdown of RPL34 in Saos-2 cells via lentivirus-mediated small interfering RNA (siRNA) significantly inhibited cell proliferation, induced cell apoptosis and G2/M phase arrest. Moreover, screening of transcription factors using University of California Santa Cruz (UCSC) Genome Browser, protein-protein interaction (PPI) network analysis, Gene Ontology (GO) and pathway enrichment analysis revealed that MYC participates in the transcriptional regulation of RPL34, which interacts with the subunits of eukaryotic translation initiation factor 3 (eIF3) and probably involves the translational control of growth-promoting proteins. Our findings suggest that RPL34 plays an important role in the proliferation of osteosarcoma cells.

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Section: Discussionmentioning

confidence: 99%

Highly expressed ribosomal protein L34 indicates poor prognosis in osteosarcoma and its knockdown suppresses osteosarcoma proliferation probably through translational control

Luo

Zhao

Fowdur

et al. 2016

Sci Rep

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“…RPL7a interacts with the human thyroid hormone receptor and inhibits transactivation. Hyperthyroidism favours osteosarcoma cell growth and down-regulation of RPL7a might enhance sensitivity to TR and disrupt growth control [61]. RPL18 was shown to inhibit autophosphorylation of the double-stranded RNA-activated protein kinase (PKR) and PKR mediated phosphorylation of the translation initiation factor eIF2α.…”

Section: Discussionmentioning

confidence: 99%

Novel polysome messages and changes in translational activity appear after induction of adipogenesis in 3T3-L1 cells

et al. 2012

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BackgroundControl of translation allows for rapid adaptation of the cell to stimuli, rather than the slower transcriptional control. We presume that translational control is an essential process in the control of adipogenesis, especially in the first hours after hormonal stimulation. 3T3-L1 preadipocytes were cultured to confluency and adipogenesis was induced by standard protocols using a hormonal cocktail. Cells were harvested before and 6 hours after hormonal induction. mRNAs attached to ribosomes (polysomal mRNAs) were separated from unbound mRNAs by velocity sedimentation. Pools of polysomal and unbound mRNA fractions were analyzed by microarray analysis. Changes in relative abundance in unbound and polysomal mRNA pools were calculated to detect putative changes in translational activity. Changes of expression levels of selected genes were verified by qPCR and Western blotting.ResultsWe identified 43 genes that shifted towards the polysomal fraction (up-regulated) and 2 genes that shifted towards free mRNA fraction (down-regulated). Interestingly, we found Ghrelin to be down-regulated. Up-regulated genes comprise factors that are nucleic acid binding (eIF4B, HSF1, IRF6, MYC, POLR2a, RPL18, RPL27a, RPL6, RPL7a, RPS18, RPSa, TSC22d3), form part of ribosomes (RPL18, RPL27a, RPL6, RPL7a, RPS18, RPSa), act on the regulation of translation (eIF4B) or transcription (HSF1, IRF6, MYC, TSC22d3). Others act as chaperones (BAG3, HSPA8, HSP90ab1) or in other metabolic or signals transducing processes.ConclusionsWe conclude that a moderate reorganisation of the functionality of the ribosomal machinery and translational activity are very important steps for growth and gene expression control in the initial phase of adipogenesis.

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“…The downregulation of L41 mRNA was detected in 75% of primary breast cancers . Downregulation of L7a is associated with a poorer survival of osteocarcinoma patients with lung metastasis . The S6 protein is highly expressed in diffuse large B‐cell lymphomas (DLBCL), and knockdown of S6 leads to decreased proliferation of these cells in culture .…”

Section: The Potential Value Of Ribosomal Proteins In the Diagnosis Amentioning

confidence: 99%

Ribosomal Proteins and Human Diseases: Pathogenesis, Molecular Mechanisms, and Therapeutic Implications

Wang

Nag

Zhang

et al. 2014

Medicinal Research Reviews

184

154

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Ribosomes are essential components of the protein synthesis machinery. The process of ribosome biogenesis is well organized and tightly regulated. Recent studies have shown that ribosomal proteins (RPs) have extraribosomal functions that are involved in cell proliferation, differentiation, apoptosis, DNA repair, and other cellular processes. The dysfunction of RPs has been linked to the development and progression of hematological, metabolic, and cardiovascular diseases and cancer. Perturbation of ribosome biogenesis results in ribosomal stress, which triggers activation of the p53 signaling pathway through RPs-MDM2 interactions, resulting in p53-dependent cell cycle arrest and apoptosis. RPs also regulate cellular functions through p53-independent mechanisms. We herein review the recent advances in several forefronts of RP research, including the understanding of their biological features and roles in regulating cellular functions, maintaining cell homeostasis, and their involvement in the pathogenesis of human diseases. We also highlight the translational potential of this research for the identification of molecular biomarkers, and in the discovery and development of novel treatments for human diseases.

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Down-regulation of ribosomal protein L7A in human osteosarcoma

Abstract: Under-expression of RPL7A may be involved in the carcinogenesis of osteosarcoma. Loss of RPL7A expression may be associated with poor survival of osteosarcoma patients with lung metastasis.

Cited by 25 publications

References 31 publications

Highly expressed ribosomal protein L34 indicates poor prognosis in osteosarcoma and its knockdown suppresses osteosarcoma proliferation probably through translational control

Highly expressed ribosomal protein L34 indicates poor prognosis in osteosarcoma and its knockdown suppresses osteosarcoma proliferation probably through translational control

Novel polysome messages and changes in translational activity appear after induction of adipogenesis in 3T3-L1 cells

Ribosomal Proteins and Human Diseases: Pathogenesis, Molecular Mechanisms, and Therapeutic Implications

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