Down-regulation of surface major histocompatibility complex class I by guinea pig cytomegalovirus

Lacayo, Juan; Sato, Hiroshi; Kamiya, Hajime; McVoy, Michael A.

doi:10.1099/vir.0.18675-0

Cited by 1 publication

(2 citation statements)

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“…GPL cells were infected with either WT-GFP or 3DX-GFP (MOI=0.1) viruses and were analyzed for class I surface expression at 72 h post infection as described previously [51] except that the murine monoclonal antibody B640, a gift from Hubert Schafer, was used to detect guinea pig MHC I (1/100 dilution). For MHC I down regulation analysis a GFP-positive wild-type GPCMV was used as a control [51].…”

Section: Methodsmentioning

confidence: 99%

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A live guinea pig cytomegalovirus vaccine deleted of three putative immune evasion genes is highly attenuated but remains immunogenic in a vaccine/challenge model of congenital cytomegalovirus infection

Crumpler

Choi

McVoy

et al. 2009

Vaccine

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Live attenuated vaccines for prevention of congenital cytomegalovirus infections encode numerous immune evasion genes. Their removal could potentially improve vaccine safety and efficacy. To test this hypothesis, three genes encoding MHC class I homologs (presumed NK evasins) were deleted from the guinea pig cytomegalovirus genome and the resulting virus, 3DX, was evaluated as a live attenuated vaccine in the guinea pig congenital infection model. 3DX was attenuated in vivo but not in vitro. Vaccination with 3DX produced elevated cytokine levels and higher antibody titers than wild type virus (WT) while avidity and neutralizing titers were similar. Protection, assessed by maternal viral loads and pup mortality following pathogenic viral challenge during pregnancy, was comparable between 3DX and WT and significant compared to naïve animals. These results suggest that the safety and perhaps efficacy of live attenuated human cytomegalovirus vaccines could be enhanced by deletion of viral immunomodulatory genes.

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Section: Methodsmentioning

confidence: 99%

“…For MHC I down regulation analysis a GFP-positive wild-type GPCMV was used as a control [51]. Mouse IgG isotype control (Biolegend) was used as a control for B640 MHC I antibody.…”

Section: Methodsmentioning

confidence: 99%