Down-regulation of TCF21 by hypermethylation induces cell proliferation, migration and invasion in colorectal cancer

Dai, Yaozhang; Duan, Hong; Duan, Chaojun; Zhou, Rongrong; He, Yuxiang; Tu, Qingsong; Shen, Liangfang

doi:10.1016/j.bbrc.2015.09.109

Cited by 30 publications

(39 citation statements)

References 23 publications

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“…TCF21 genetic polymorphisms are associated with the risk of breast cancer in Chinese women 6. Our previous study has revealed that TCF21 is frequently silenced by promoter hypermethylation in both CRC cell lines and primary CRC and that TCF21 methylation is significantly correlated with lymph node invasion 7. However, the role of TCF21 in CRC and the underlying mechanism remain unclear.…”

Section: Introductionmentioning

confidence: 99%

TCF21 functions as a tumor suppressor in colorectal cancer through inactivation of PI3K/AKT signaling

Dai¹,

Duan²,

Duan³

et al. 2017

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Colorectal cancer (CRC) has become a major public health problem, ranking as the third most common type of cancer. Our previous study has revealed that TCF21 is frequently silenced by promoter hypermethylation in both CRC cell lines and primary CRC, with TCF21 methylation being significantly correlated with lymph node invasion. In this study, we further analyze the expression of TCF21 in CRC tissues and investigate the role of TCF21 in CRC in vitro and in vivo. We also explore the possible pathway regulated by TCF21. We thus demonstrate that decreased levels of TCF21 are associated with the pathological stage, clinical stage and lymph node metastasis, indicating a poor prognosis in CRC patients; overexpression of TCF21 inhibits cell proliferation, migration and invasion in the colorectal cell lines HCT116 and HT29. Furthermore, TCF21 functions as a tumor suppressor probably through inactivation of PI3K/AKT signaling and inhibition of MMPs. Our results suggest that enhancement of TCF21 levels may be a potential strategy to facilitate the prevention and treatment of CRC in the clinic.

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Section: Introductionmentioning

confidence: 99%

TCF21 functions as a tumor suppressor in colorectal cancer through inactivation of PI3K/AKT signaling

Dai¹,

Duan²,

Duan³

et al. 2017

OTT

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“…Furthermore, TCF21 activity was restored with demethylation of CRC cell lines, inhibiting cell growth and invasion and promoting apoptosis. These results indicate that the TCF21 gene may serve as a tumor suppressor [53].…”

Section: Tcf21mentioning

confidence: 75%

Updates on the Molecular Genetics of Colorectal Cancer

Wong¹,

Xie²

2017

Colorec Cancer

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Colorectal cancer (CRC) is a leading cause of cancer mortality worldwide. Although definitive therapies for advanced disease are still lacking, rapid progress has been made in the last decade in understanding the molecular mechanisms underlying CRC tumorigenesis and progression. In this review, we summarize the most recent findings in the molecular genetics of CRC with a focus on gene mutations and epigenetic changes that were identified in CRC patients.

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“…Curcumin abrogates the catalytic activity of a methyltransferase, DNMT1, thereby affecting the methylation of a number of cancer-related genes [8–11]. TCF21, which can be inhibited by methylation, promotes mesenchymal to epithelial transition (MET) during organogenesis [12]. The reversal of MET, epithelial-to-mesenchymal transition (EMT), is a primary driver for cancer progression.…”

Section: Introductionmentioning

confidence: 99%

Curcumin increases exosomal TCF21 thus suppressing exosome-induced lung cancer

Zhou

Zeng

et al. 2016

Oncotarget

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Curcumin is a novel drug for lung cancer treatment. However, the mechanism underlying the anti-tumor effect of curcumin remains elusive. Previous evidences indicated that, the methylating transferase DNMT1 is downregulated by curcumin, and the transcription factor 21 (TCF21) is suppressed by DNMT1. We hereby attempt to elucidate the correlation between curcumin treatment and TCF21 expression. Exosomes derived from curcumin-pretreated H1299 cells were used to treat BEAS-2B cells, which induced proliferation, colony formation and migration of BEAS-2B cells. An increase in TCF21 expression in response to curcumin was also seen, as revealed by real-time PCR (RT-PCR) and western blot. Analysis using the GEO database (access #GSE21210) indicated that a positive correlation existed between TCF21 levels and lung cancer patient survival. TCF21 overexpression and knockdown was introduced to H1299 cells through lentiviral system, which led to suppression and promotion of tumor growth, respectively. We also demonstrated that DNMT1 expression was downregulated by curcumin. Therefore, curcumin exerts its anti-cancer function by downregulating DNMT1, thereby upregulating TCF21.

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Down-regulation of TCF21 by hypermethylation induces cell proliferation, migration and invasion in colorectal cancer

Cited by 30 publications

References 23 publications

TCF21 functions as a tumor suppressor in colorectal cancer through inactivation of PI3K/AKT signaling

TCF21 functions as a tumor suppressor in colorectal cancer through inactivation of PI3K/AKT signaling

Updates on the Molecular Genetics of Colorectal Cancer

Curcumin increases exosomal TCF21 thus suppressing exosome-induced lung cancer

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