2006
DOI: 10.1002/path.1922
|View full text |Cite
|
Sign up to set email alerts
|

Down-regulation of the claudin-18 gene, identified through serial analysis of gene expression data analysis, in gastric cancer with an intestinal phenotype

Abstract: Gastric cancer (GC) is one of the most common malignancies worldwide. Genes whose expression is down-regulated in GC may be tumour suppressor genes. In the present study, genes with decreased expression in GC were screened for by serial analysis of gene expression (SAGE) data analysis and reverse transcription (RT)-polymerase chain reaction (PCR), and CLDN18 (encoding claudin-18) was identified. Quantitative RT-PCR revealed that expression of CLDN18 was down-regulated in 13 (56.5%) of 23 GCs. Immunostaining sh… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

10
120
4
3

Year Published

2007
2007
2016
2016

Publication Types

Select...
8
1

Relationship

0
9

Authors

Journals

citations
Cited by 146 publications
(137 citation statements)
references
References 21 publications
10
120
4
3
Order By: Relevance
“…However, apart from E-cadherin, only few studies investigated the correlation of TJ proteins with gastric cancer prognosis: reduced expression of claudin 3 has been associated with a poorer prognosis in intestinal-type tumours, yet failing to gain statistical significance in multivariate analysis (Soini et al, 2006). Furthermore, gastric cancer patients showing downregulation of claudin 18 had a significantly worse survival, compared with patients with robust expression of this protein (Sanada et al, 2006). In contrast, strong expression of claudin 4 significantly correlated with a decreased survival in gastric cancers (Resnick et al, 2005).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…However, apart from E-cadherin, only few studies investigated the correlation of TJ proteins with gastric cancer prognosis: reduced expression of claudin 3 has been associated with a poorer prognosis in intestinal-type tumours, yet failing to gain statistical significance in multivariate analysis (Soini et al, 2006). Furthermore, gastric cancer patients showing downregulation of claudin 18 had a significantly worse survival, compared with patients with robust expression of this protein (Sanada et al, 2006). In contrast, strong expression of claudin 4 significantly correlated with a decreased survival in gastric cancers (Resnick et al, 2005).…”
Section: Discussionmentioning
confidence: 99%
“…Although previously, in particular, the adherens junction protein E-cadherin (Christofori and Semb, 1999) has been studied, investigation of tight junctions (TJs) in gastric cancer has become of interest in recent years. Hereby, a decreased presence of TJ proteins has been described for claudins 4, 18, and 23, ZO-1 as well as occludin, in part correlating with poor cancer differentiation (Kimura et al, 1997;Katoh, 2005;Lee et al, 2005;Sanada et al, 2006). An increased presence of TJ proteins when compared with normal gastric mucosa has been described for claudins 1, 3, 4, 5, and 7, particularly in intestinal-type adenocarcinomas (Johnson et al, 2005;Resnick et al, 2005;Hewitt et al, 2006;Soini et al, 2006;Wu et al, 2006).…”
mentioning
confidence: 99%
“…Atrophic and intestinal metaplasia induced by Helicobacter pylori infection changes the TJ protein expression pattern from that of gastric-type claudins to that of intestinal-type claudins [50,[52][53][54][55]. Therefore, the interpretation of an increase or decrease in TJ proteins in cancer should be carefully analyzed, and may be primarily associated with the phenotype change of gastric epithelial cells rather than with cancer development or progression.…”
Section: Gastric Cancermentioning
confidence: 99%
“…24,37 In contrast, CLDN18 expression was significantly downregulated in gastric carcinoma as reported previously. 38 On the basis of these microarray data, the protein levels of claudin-4 expression were further determined in normal gastric mucosa, gastric adenocarcinoma and its related lesions using immunohistochemistry. Claudin-4 was strongly stained in both cytoplasm and membrane in intestinal metaplasia and gastric epithelial dysplasia lesions, whereas normal gastric epithelia and chronic gastritis samples were focally and weakly stained for claudin-4 ( Figure 1a, Supplementary Table S3).…”
Section: Increased Membranous Claudin-4 Expression In Gastric Adenocamentioning
confidence: 99%