Down-regulation of the cotton endo-1,4-β-glucanase gene KOR1 disrupts endosperm cellularization, delays embryo development, and reduces early seedling vigour

Shang, Xiaoguang; Chai, Qichao; Qing-hu, Zhang; Jiang, Jianxiong; Zhang, Tianzhen; Guo, Wangzhen; Ruan, Yong‐Ling

doi:10.1093/jxb/erv111

Cited by 18 publications

(8 citation statements)

References 45 publications

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“…The total RNA was extracted from different tissues, including roots, stems, and leaves using the cetyltrimethyl ammonium bromide-acid phenol method (Shang et al, 2015). First-strand cDNA was generated using HiScript Q RT SuperMix for qPCR (+gDNA wiper) (Vazyme) according to the manufacturer's instructions.…”

Section: Rna Extraction and Qrt-pcrmentioning

confidence: 99%

5-Aminolevulinic Acid Dehydratase Gene Dosage Affects Programmed Cell Death and Immunity

Chai

Shang

et al. 2017

Plant Physiol.

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Programmed cell death (PCD) is an important form to protect plants from pathogen attack. However, plants must precisely control the PCD process under microbe attacks to avoid detrimental effects. The complexity of how plants balance the defense activation and PCD requires further clarification. Lesion mimic mutants constitute an excellent material to study the crosstalk between them. Here, we identified a (cotton) lesion mimic mutant (), which exhibits necrotic leaf damage and enhanced disease resistance. Map-based cloning demonstrated that , encoding 5-aminolevulinic acid dehydratase and located on chromosome D5, was responsible for the phenotype. The mutant was resulted from a nonsense mutation within the coding region of It exhibited an overaccumulation of the 5-aminolevulinic acid, elevated levels of reactive oxygen species and salicylic acid, along with constitutive expression of pathogenesis-related genes and enhanced resistance to the infection. Interestingly, plays a dosage-dependent role in regulating PCD of cotton leaves and resistance to infection. This study provides a new strategy on the modulation of plant immunity, particularly in polyploidy plants.

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Section: Rna Extraction and Qrt-pcrmentioning

confidence: 99%

5-Aminolevulinic Acid Dehydratase Gene Dosage Affects Programmed Cell Death and Immunity

Chai

Shang

et al. 2017

Plant Physiol.

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“…Recent RNAi and co-suppression mediated down regulation study toward investigating the potential function of cotton KORRIGAN ( GhKOR1 ), a highly conserved membrane-bound endoglucanase, has resulted in smaller filial tissue and reduced seed weight, leading to various abnormalities in endosperm formation and delayed embryo development. Results collectively suggested that the GhKOR1 gene is required for the development of viable cottonseed with normal seed germination and seedling growth ( Shang et al, 2015 ). Another interesting RNAi gene silencing study targeting cotton acyl-CoA N-acyltransferase (EC 2.3; GhACNAT ) using VIGS resulted in abnormal anther formation and sterile plants.…”

Section: Rnai For Fertility and Embryogenesis Studiesmentioning

confidence: 99%

RNA Interference for Functional Genomics and Improvement of Cotton (Gossypium sp.)

Abdurakhmonov¹,

Ayubov²,

Ubaydullaeva³

et al. 2016

Front. Plant Sci.

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RNA interference (RNAi), is a powerful new technology in the discovery of genetic sequence functions, and has become a valuable tool for functional genomics of cotton (Gossypium sp.). The rapid adoption of RNAi has replaced previous antisense technology. RNAi has aided in the discovery of function and biological roles of many key cotton genes involved in fiber development, fertility and somatic embryogenesis, resistance to important biotic and abiotic stresses, and oil and seed quality improvements as well as the key agronomic traits including yield and maturity. Here, we have comparatively reviewed seminal research efforts in previously used antisense approaches and currently applied breakthrough RNAi studies in cotton, analyzing developed RNAi methodologies, achievements, limitations, and future needs in functional characterizations of cotton genes. We also highlighted needed efforts in the development of RNAi-based cotton cultivars, and their safety and risk assessment, small and large-scale field trials, and commercialization.

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“…Aniline blue was prepared in water at 0.05% (w/v) and stored in the dark at 4°C. Free-hand sections (;1 mm) of seeds were produced and stained with aniline blue for 10 min in the dark, followed by a 30-s wash with water (Shang et al, 2015). Fluorescent signals from aniline blue were observed under a Zeiss LSM780 confocal microscope using a 405-nm excitation laser (Benitez-Alfonso et al, 2013).…”

Section: Aniline Blue Staining Electron Microscopy Of Pd Structure mentioning

confidence: 99%

“…Seeds fixation and embedding were performed as described by Shang et al (2015). Cotton seeds (5 DPA) were fixed in 2% (v/v) paraformaldehyde and 0.1% (v/v) glutaraldehyde in phosphate buffer (25 mM, pH 7.2) for 2 h. The seeds were dehydrated in a step-graded ethanol series and embedded in LR White resin (medium grade; Alltech) through a step-graded series after washing in buffer.…”

Section: Aniline Blue Staining Electron Microscopy Of Pd Structure mentioning

confidence: 99%

Suppressing a Putative Sterol Carrier Gene Reduces Plasmodesmal Permeability and Activates Sucrose Transporter Genes during Cotton Fiber Elongation

et al. 2017

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Plasmodesmata (PDs) play vital roles in cell-to-cell communication and plant development. Emerging evidence suggests that sterols are involved in PD activity during cytokinesis. However, whether sterols contribute to PD gating between established cells remains unknown. Here, we isolated , a putative sterol carrier protein gene from elongating cotton () fibers. In contrast to wild-type fiber PDs, which opened at 5 to 10 d postanthesis (DPA) and closed only at 15 to 25 DPA, plants with suppressed expression had reduced sterol contents and closed PDs at 5 through 25 DPA Thesuppressed fibers exhibited callose deposition at the PDs, likely due to reduced expression of , which encodes a PD-targeting β-1,3-glucanase. Both expression and callose deposition were sensitive to a sterol biosynthesis inhibitor. Moreover, suppressing upregulated a cohort of and sucrose transporter genes in fiber cells. Collectively, our results indicate that (1) GhSCP2D is required for expression to degrade callose at the PD, thereby contributing to the establishment of the symplasmic pathway; and (2) blocking the symplasmic pathway by downregulating activates or increases the expression of and , leading to the switch from symplasmic to apoplasmic pathways.

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Down-regulation of the cotton endo-1,4-β-glucanase gene KOR1 disrupts endosperm cellularization, delays embryo development, and reduces early seedling vigour

Abstract: HighlightTransgenic evidence is provided that the expression of a cotton endo-1,4-β-glucanase, GhKOR1, is required for endosperm cellularization, cotton fibre cellulose synthesis, and the establishment of seedling vigour

Cited by 18 publications

References 45 publications

5-Aminolevulinic Acid Dehydratase Gene Dosage Affects Programmed Cell Death and Immunity

5-Aminolevulinic Acid Dehydratase Gene Dosage Affects Programmed Cell Death and Immunity

RNA Interference for Functional Genomics and Improvement of Cotton (Gossypium sp.)

Suppressing a Putative Sterol Carrier Gene Reduces Plasmodesmal Permeability and Activates Sucrose Transporter Genes during Cotton Fiber Elongation

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