Down‐regulation of the microRNA processing enzyme Dicer is a prognostic factor in human colorectal cancer

Faggad, Areeg; Kasajima, Atsuko; Weichert, Wilko; Stenzinger, Albrecht; Elwali, Nasr Eldin; Dietel, Manfred; Denkert, Carsten

doi:10.1111/j.1365-2559.2011.04110.x

Cited by 45 publications

(39 citation statements)

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“…We have demonstrated significant correlations between the DICER1 mRNA and tumor size, depth of invasion, lymph node metastasis, lymphatic invasion and Dukes' stage. Using the IHC method, Faggad et al (23) reported that the DICER1 protein expression in CRC tissues showed a significant correlation with tumor grade, lymph node metastasis, localization of tumor and tumor stage, thereby partially supporting our findings. We also evaluated the prognostic value of DICER1 mRNA in CRC tissues.…”

Section: Univariate Analysis Multivariate Analysis ------------------supporting

confidence: 79%

“…By contrast, Faggad et al (23) reported that the downregulation of DICER1 is a prognostic factor in CRC patients with WHO stage I, II, III and IV. These studies examined the DICER1 protein levels of tissue microarrays (TMA) using IHC staining.…”

Section: Univariate Analysis Multivariate Analysis ------------------mentioning

confidence: 98%

“…Therefore, it is of interest to determine whether or not DICER1 may be used as a prognostic marker to predict an individual patient's risk. Using the immunohistochemical method (IHC), only a few clinical studies have investigated the usefulness of DICER1 protein levels as a prognostic factor in CRC patients (22,23). However, the prognostic values of DICER1 protein levels in these studies were contradictory.…”

Section: Introductionmentioning

confidence: 99%

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Clinicopathological and prognostic significance of the microRNA processing enzyme DICER1 mRNA expression in colorectal cancer patients

Akahane

2012

Molecular and Clinical Oncology

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Section: Univariate Analysis Multivariate Analysis ------------------supporting

confidence: 79%

Section: Univariate Analysis Multivariate Analysis ------------------mentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Clinicopathological and prognostic significance of the microRNA processing enzyme DICER1 mRNA expression in colorectal cancer patients

Akahane

2012

Molecular and Clinical Oncology

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“…Furthermore, the upregulated expression of Dicer in serous ovarian carcinoma is associated with advanced tumor stages (25) and its downregulated expression in breast cancer is associated with cancer progression and recurrence (26). Particularly in CRC, previous studies have identified that dysregulation of the miRNA machinery may be involved in carcinogenesis (10,14,16,27,28). Drosha mRNA levels were not identified to be significantly associated with tumor stages (10).…”

Section: Discussionmentioning

confidence: 71%

miRNA biogenesis-associated RNase III nucleases Drosha and Dicer are upregulated in colorectal adenocarcinoma

et al. 2017

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Abstract. Drosha and Dicer are important regulators of microRNA (miRNA) biogenesis, and it has been suggested that their aberrant regulation may cause colorectal cancer (CRC). The aim of the present study was to evaluate the mRNA expression levels of these two important RNase III nucleases and their association with clinical features in CRC specimens from South Korean patients. The expression levels of Drosha and Dicer mRNA were investigated in 77 CRC tissues and adjacent histologically non-neoplastic tissues using the quantitative polymerase chain reaction. The expression levels of Drosha and Dicer mRNA were identified to be upregulated in CRC. Neither the Drosha nor the Dicer mRNA expression level was associated with any clinical parameter, including sex, age, TNM stage, body mass index and carcinoembryonic antigen titer in patients with CRC. Furthermore, the expression levels of Drosha and Dicer mRNA were not associated with each other. The miRNA biogenesis-associated RNase III nucleases Drosha and Dicer are significantly upregulated in CRC, suggesting their importance in the pathobiology of colorectal carcinogenesis. IntroductionColorectal carcinoma (CRC) is the third most common cancer in Western countries and South Korea (1,2). CRC is also one of the primary causes of cancer mortality worldwide (1,3). The pathogenesis of CRC has been reported to be complicated and tightly controlled by various mechanisms, including genome structural rearrangements, chromatin remodeling, genetic mutations and epigenetic alterations (4,5).MicroRNAs (miRNAs), which are small RNA molecules that serve an essential role in fine-tuning gene expression, regulate a range of biological processes, including cellular development, differentiation, proliferation, K + channel modulation, stress responses, DNA repair, cell adhesion, cell death, inflammation, metabolism and tumor development (6-9). It has been identified that appropriate and physiological miRNA biogenesis is controlled by an elaborate and well-regulated process, referred to as the 'miRNA machinery' pathway (10). This signaling pathway is regulated by various components, including Drosha, DiGeorge syndrome critical region gene 8 (DGCR8), exportin-5 (Xpo5), Dicer, transactivation-responsive RNA-binding protein (TRBP) and Argonaute (AGO). Among these, Drosha and Dicer are important regulators of miRNA biogenesis. In the nucleus, primary miRNAs (RNA extensions several hundred nucleotides long) are processed into precursor miRNAs (pre-miRNAs; stem-loop structures of between 70 and 100 nucleotides) by RNase III Drosha (11). These pre-miRNAs are then processed by another RNase III, Dicer, into mature miRNAs within the cytoplasm (12).As disruption of the miRNA machinery pathway has been suggested to be involved in cancer (13), a number of studies have demonstrated the expression and the clinical implications of the components of the miRNA machinery pathway in CRC. For instance, Faber et al (14) identified that overexpressed Dicer is significantly associated with poor survival and...

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“…Global repression of miRNAs in human cancers has been observed in previous studies (11,12) and has been suggested to result from defective biogenesis machinery. Dysregulation of Dicer is of great interest because, similarly to the suppression of miRNA, downregulation of Dicer has been widely observed in many types of human tumors and Dicer has been shown to function as a tumor suppressor (12)(13)(14)(15)(16)(17). Suppression of Dicer expression was found to dramatically enhance cancer metastasis through miR-200 miRNA (15,18).…”

Section: Introductionmentioning

confidence: 99%

HIF-1α promotes autophagic proteolysis of Dicer and enhances tumor metastasis

Lai¹,

Li²,

Wang³

et al. 2017

Journal of Clinical Investigation

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Down‐regulation of the microRNA processing enzyme Dicer is a prognostic factor in human colorectal cancer

Abstract: Dicer deregulation is linked significantly to adverse disease state and decreased overall survival in colorectal cancer. Our data suggest that reduced Dicer expression might contribute to tumour progression in colorectal cancer.

Cited by 45 publications

References 50 publications

Clinicopathological and prognostic significance of the microRNA processing enzyme DICER1 mRNA expression in colorectal cancer patients

Clinicopathological and prognostic significance of the microRNA processing enzyme DICER1 mRNA expression in colorectal cancer patients

miRNA biogenesis-associated RNase III nucleases Drosha and Dicer are upregulated in colorectal adenocarcinoma

HIF-1α promotes autophagic proteolysis of Dicer and enhances tumor metastasis

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