Down-regulation of β-carotene hydroxylase increases β-carotene and total carotenoids enhancing salt stress tolerance in transgenic cultured cells of sweetpotato

Kim, Sun Ha; Ahn, Young Ock; Ahn, Mi‐Jeong; Lee, Haeng‐Soon; Kwak, Sang‐Soo

doi:10.1016/j.phytochem.2011.11.003

Cited by 137 publications

(109 citation statements)

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“…In addition, beta-carotene as one of defense chemicals has been reported could be distinctly increased under biotic and abiotic environmental stresses in plants (Rosenfeld et al 1998;Du et al 2010;Kim et al 2012b;Wang et al 2014). Therefore, it is worth noting that amounts of endogenous beta-carotene would be favorable for strengthening the plant defense system under biotic stresses, such as virus attacks.…”

Section: Discussionmentioning

confidence: 99%

“…In plants, several studies suggested that the antioxidant activity of plants under salt, high temperature, drought, and pathogen stress could be enhanced by increasing their beta-carotene, vitamin C, and lycopene content (Rosenfeld et al 1998;Du et al 2010;Kim et al 2012b;Wang et al 2014). Recent years, transgenic plants have been developed by metabolic engineering to enhance the production of carotenoids, such as beta-carotene (pro-vitamin A), vio-laxanthin, and zeaxanthin, thereby increasing the nutrient value and stress tolerance of plants.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of the Response of Beta Carotene Enhanced Transgenic Soybeans to Soybean Mosaic Virus (SMV)

Yang

Shin

Seo

et al. 2016

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Beta-carotene, a defense chemical, is synthesized by the carotenoid biosynthesis pathway. In the present study, a transgenic soybean line, with a single copy insertion of phytoene synthase and carotene desaturase genes, having high beta-carotene content was studied for its response to systemically inoculated Soybean mosaic virus (SMV). Beta-carotene-enhanced transgenic soybean showed similar leaf and seed symptoms, viral RNA, and protein expression compared to the non-genetically modified (GM) 'Kwangan' control. Total antioxidant contents in the non-GM 'Kwangan' line were increased after SMV attack in both leaves and seeds; however, the antioxidant contents in the beta-carotene-enhanced soybean line have no significant changes. In addition, both GM and non-GM soybean were detected increased lipid hydroperoxide concentrations in leaves and seeds after SMV infection, even though they did not reach a statistical significant level. Abscisic acid (ABA) levels in beta-carotene-enhanced transgenic soybean seeds was determined 35-fold increase after SMV infections caused a lower seed germination rate and a higher SMV transmission rate to subsequent generations, compared to those of non-GM 'Kwangan'. Thus, we concluded that the additional production of beta-carotene did not confer resistance of beta-carotene-enhanced transgenic soybean to SMV infections, but caused mass accumulations of ABA in seeds.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assessment of the Response of Beta Carotene Enhanced Transgenic Soybeans to Soybean Mosaic Virus (SMV)

Yang

Shin

Seo

et al. 2016

Plant Breed. Biotech.

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“…Although sweetpotato is highly important as a valuable source of carotenoids including β-carotene, very little research has been done on molecular biological aspects of its carotenoid biosynthesis [6,7,9]. 'White Star' (WS) and W71, producing white-and orange-fleshed tubers, respectively, are important sweetpotato cultivars, since they are amenable to Agrobacterium-mediated transformation ( [13,14] and our unpublished results).…”

Section: Introductionmentioning

confidence: 95%

“…It can be used as food supply to combat malnutrition in the developing nations, since the tuberous roots (tubers) are enriched with starch and dietary fiber, along with carotenoids, anthocyanin, ascorbic acid, potassium, calcium, iron, and other bioactive ingredients [2][3][4][5]. Sweetpotato also possesses a potential for bioenergy production as it can adapt to growth on marginal lands [6]. For people of South-east Asia and Africa, this crop is the main source of β-carotene [7].…”

Section: Introductionmentioning

confidence: 99%

Carotenoid analysis of sweetpotatoIpomoea batatasand functional identification of its lycopene β- and ε-cyclase genes

Khan

Takemura

Maoka

et al. 2016

Zeitschrift Für Naturforschung C

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Abstract:Sweetpotato Ipomoea batatas is known as a hexaploid species. Here, we analyzed carotenoids contained in the leaves and tubers of sweetpotato cultivars 'White Star' (WS) and W71. These cultivars were found to contain several carotenoids unique to sweetpotato tubers such as β-carotene-5,6,5′,8′-diepoxide and β-carotene-5,8-epoxide. Next, we isolated two kinds of carotene cyclase genes that encode lycopene β-and ε-cyclases from the WS and W71 leaves, by RT-PCR and subsequent RACE. Two and three lycopene β-cyclase gene sequences were, respectively, isolated from WS, named IbLCYb1, 2, and from W71, IbLCYb3, 4, 5. Meanwhile, only a single lycopene ε-cyclase gene sequence, designated IbLCYe, was isolated from both WS and W71. These genes were separately introduced into a lycopene-synthesizing Escherichia coli transformed with the Pantoea ananatis crtE, crtB and crtI genes, followed by HPLC analysis. β-Carotene was detected in E. coli cells that carried IbLCYb1-4, indicating that the IbLCYb1-4 genes encode lycopene β-cyclase. Meanwhile, the introduction of IbLCYe into the lycopene-synthesizing E. coli led to efficient production of δ-carotene with a monocyclic ε-ring, providing evidence that the IbLCYe gene codes for lycopene ε-(mono)cyclase. Expression of the β-and ε-cyclase genes was analyzed as well.Keywords: α-carotene; β-carotene; carotenoid; Ipomoea batatas; lycopene cyclase; sweetpotato.Dedication: This work is dedicated to the memory of Professor Peter BÖger. He kindly provided the opportunity for NM to work in his laboratory on the generation of bleaching herbicide-resistant plants, and encouraged NM to work on carotenoid biosynthesis.

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“…Biofortification of beta carotene on plants can be conducted through several approaches, for instance by down regulating the gene encoding the beta carotene hydroxilase or isopentenyl diphosphate isomerase, overexpression of the gene 1 deoxy D-xylulose 5 phosphate synthase (DXS) and gene pythoene synthase (McGregor and Labonte 2006;Telengech et al 2015). Beta carotene hydroxilase are regulator enzymes that catalyze the hydroxylation process of beta carotene into beta cryptosantin and of beta cryptosantin into zea xanthine (Kim et al 2012). The accumulation process of provitamin A on cassava tuber (Manihot esculenta Cranz) is controlled by a single nucleotide polymorphism (SNP) of phytoene synthase gene (Welsch et al 2010;Welsch 2011), therefore, overexpression of phytoene synthase genes could increase beta-carotene levels on cassava.…”

Section: Introductionmentioning

confidence: 99%

Technological innovation in the protection of beta carotene on MOCAF production which is rich in beta carotene

et al. 2017

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Abstract. Hartati, Fathoni A, Kurniawati S, Hartati NS, Sudarmonowati E. 2017. Technological innovation in the protection of beta carotene on MOCAF production which is rich in beta carotene. Nusantara Bioscience 9: 6-11. The conversion process of high beta carotene cassava into high beta carotene MOCAF might cause a high loss of beta carotene contents during the process of flour making. This research aims to develop the most appropriate technology to protect the loss of beta carotene contents during MOCAF process. Preservation methods used consisting of four starter treatment combinations of Bimo-CF and sodium metabisulfite (Na 2 S 2 O 5 ) on Adira 1 variety, namely (i) 0.5 g starter/L volume of soaking solution and 0.3% of Na 2 S 2 O 5 , (ii) 0.5 g starter and 0.15% of Na 2 S 2 O 5 , (iii) 1 g starter and 0.3% of Na 2 S 2 O 5 , and (iv) 1 g starter and 0.15% of Na 2 S 2 O 5 . The results showed that Na 2 S 2 O 5 can reduce the loss of beta carotene contents during the process of flour making. Of the initial concentration of beta carotene in tubers (3.5 mg/kg), the loss of beta carotene on MOCAF control without preservation process with Na 2 S 2 O 5 reached 68.8% (1.09 mg/kg). With Na 2 S 2 O 5 application, the loss of beta carotene could be reduced to 24.3% (2.65 mg/kg). The fermentation process with Bimo-CF starter increased the protein content of MOCAF two times from the control. The best and safest protection of beta carotene for food was achieved from preservation method using the combination of 1 g/L volume of soaking solution starter and 0.15% of Na 2 S 2 O 5 which produced residue of Na 2 S 2 O 5 to 53.07 mg/kg, which means lower than the maximum residue allowed in a flour product (70 mg/kg). The MOCAF yield obtained from each combination is quite high in range of 32-38%. The highest MOCAF yield is obtained from the protection method using combination of 1 g/L volume of starter soaking solution with Na 2 S 2 O 5 0% and 0.15%.

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Down-regulation of β-carotene hydroxylase increases β-carotene and total carotenoids enhancing salt stress tolerance in transgenic cultured cells of sweetpotato

Cited by 137 publications

References 45 publications

Assessment of the Response of Beta Carotene Enhanced Transgenic Soybeans to Soybean Mosaic Virus (SMV)

Assessment of the Response of Beta Carotene Enhanced Transgenic Soybeans to Soybean Mosaic Virus (SMV)

Carotenoid analysis of sweetpotatoIpomoea batatasand functional identification of its lycopene β- and ε-cyclase genes

Technological innovation in the protection of beta carotene on MOCAF production which is rich in beta carotene

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