Downregulated circular RNA hsa_circ_0001649 regulates proliferation, migration and invasion in cholangiocarcinoma cells

Xu, Yi; Yao, Yongliang; Zhong, Xian; Leng, Kaiming; Qin, Wei; Qu, Lijun; Cui, Yunfu; Jiang, Xingming

doi:10.1016/j.bbrc.2018.01.077

Cited by 155 publications

(143 citation statements)

References 26 publications

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“…CircRNAs were discovered over 30 years ago, but has only recently attracted investigators' attention due to their important functional roles in diseases [16]. Recently, accumulating evidence has indicated that circRNAs could function as a biomarker to predict the clinical severity in cancer patients [17][18][19][20]. Previously, circ_0005230 was found upregulated in BC tissues revealed by the human circRNA microarray [13].…”

Section: Discussionmentioning

confidence: 99%

“…Cell metastatic properties were determined by Transwell assays in accordance with the previous studies [14,17].…”

Section: Transwell Assaymentioning

confidence: 99%

“…The xenograft study was approved by the Animal Care and Use Committee of Harbin Medical University and conducted as previously described [14,17]. Transfected MCF7 cells were used for injection.…”

Section: Animal Studymentioning

confidence: 99%

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Increased Expression of Circular RNA circ_0005230 Indicates Dismal Prognosis in Breast Cancer and Regulates Cell Proliferation and Invasion via miR-618/ CBX8 Signal Pathway

Yao

Leng

et al. 2018

Cell Physiol Biochem

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Background/Aims: Circular RNAs (circRNAs) are a class of non-coding RNAs. They have been proved to be critically involved in tumorigenesis and progression of malignancies through competing endogenous RNA (ceRNA) mechanism. Nevertheless, the exploration between circRNAs and pathogenesis of breast cancer (BC) is limited. Previously, circ_0005230 was identified upregulated in BC tissues screened by circRNA microarray. In the present study, we aimed to investigate the expression pattern, functional role, and mechanism of circ_0005230 in BC. Methods: qRT-PCR was conducted to elucidate the expression levels of circ_0005230 in BC tissues and cells. Additionally, the clinical severity and prognostic value were investigated. CCK-8, colony-forming, flow cytometric assays were performed. Animal study was conducted to validate the in vitro data. What’s more, Transwell assays were induced to detect the cell metastatic properties of circ_0005230 exerts in BC cells. Luciferase reporter assay was used to measure the mechanism of circ_0005230. Results: circ_0005230 was overexpressed in BC tissue specimens and cell lines. The overexpression of circ_0005230 was related to adverse phenotypes in the patients with BC. In addition, circ_0005230 could be regarded as a prognostic predictor in BC patients. In vitro and in vivo data demonstrated the cell growth promoting role of circ_0005230. Moreover, circ_0005230 could also promote cell migratory and invasive capacities. For the mechanism investigation, circ_0005230 was proved to be a sponge of miR-618, and expression of miR-618 could regulate CBX8 expression via targeting the 3’UTR of CBX8. Rescue assays also illustrated an oncogenic function of circ_0005230 in BC via acting as a miR-618 sponge to promote CBX8 expression. Conclusion: circ_0005230/miR-618/CBX8 axis might play a key role in BC tumorigenesis and development.

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Section: Discussionmentioning

confidence: 99%

“…Cell metastatic properties were determined by Transwell assays in accordance with the previous studies [14,17].…”

Section: Transwell Assaymentioning

confidence: 99%

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Increased Expression of Circular RNA circ_0005230 Indicates Dismal Prognosis in Breast Cancer and Regulates Cell Proliferation and Invasion via miR-618/ CBX8 Signal Pathway

Yao

Leng

et al. 2018

Cell Physiol Biochem

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“…8,9 Emerging evidence shows that circRNA can serve as a competing endogenous RNA to modulate gene expression through sponging micro-RNAs (miRNAs). 8,10 For instance, hsa_circ_0001946 facilitates lung adenocarcinoma cell growth via affecting miR-135a-5p/SIRT1 signaling. 11 In glioma, circ_0034642 could enhance BATF3 level by targeting miR-1205 to promote cell progression.…”

Section: Introductionmentioning

confidence: 99%

Circular RNA circ_0079593 indicates a poor prognosis and facilitates cell growth and invasion by sponging miR‐182 and miR‐433 in glioma

Zhu

Liu

et al. 2019

J of Cellular Biochemistry

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Glioma is one of the major global health problems, including in China. Circular RNAs (circRNAs) have been increasingly identified and characterized in almost every aspect of biology, especially in cancer biology. This research desires to explore the functions and mechanism of a novel circRNA, circ_0079593, on regulating glioma progression. Quantitative real‐time polymerase chain reaction (qRT‐PCR) was carried out to measure the relative expression of circ_0079593, which was upregulated in matched cancerous tissues from 60 patients and four cell lines of glioma. A higher level of circ_0079593 in glioma specimens was linked to larger tumor size, higher WHO grade, and worse survival rate for patients with glioma. Moreover, circ_0079593 can be deemed as an independent prognostic predictor for glioma patients analyzed by multivariate method. Cell counting kit‐8, flow cytometric, wound healing, and transwell experiments were used to evaluate cell growth, apoptosis, migration, and invasion influenced by circ_0079593 knockdown/overexpression. Exogenous downregulation of circ_0079593 expression significantly suppressed glioma cell proliferation by increasing cell apoptosis in vitro, and retarded the migratory and invasive potential. Ectopic expressed circ_0079593 could induce the opposite effects. Mechanistically, bioinformatics analysis, qRT‐PCR, and dual‐luciferase reporter assays showed that microRNA 182 (miR‐182) and miR‐433 could be sponged and negatively regulated by circ_0079593. Further, rescue assays demonstrated that the biological functions of circ_0079593 are dependent on its inhibition of miR‐182 and miR‐433. Collectively, the present work indicates that circ_0079593 may be used as an effective prognostic marker and therapeutic target for glioma.

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“…5,6 Accumulating evidence indicates that circRNA is a promising biomarker in cancer due to its stability and specificity. 19 Circ-CDC45 (has_circ_0062270) is a novel circRNA located at chr22: 19496052-19502571. 19 Circ-CDC45 (has_circ_0062270) is a novel circRNA located at chr22: 19496052-19502571.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of circular RNA circ‐CDC45 facilitates glioma cell progression by sponging miR‐516b and miR‐527 and predicts an adverse prognosis

Liu

Hou

et al. 2019

J of Cellular Biochemistry

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Glioma (GM) is a highly lethal human cancer. Circular RNAs (circRNAs) act as an imperative factor in oncogenesis. We aimed to investigate the biological functions and mechanisms of circ‐CDC45 in GM. circ‐CDC45 expression in GM specimens and cell lines was measured by real‐time quantitative reverse transcription polymerase chain reaction. Fisher's exact test and Kaplan‐Meier curves further analyzed its clinical implications. A gain/loss‐of‐function study was conducted to investigate the role of circ‐CDC45 in GM. Additionally, luciferase reporter and rescue assays were performed to unravel the mechanisms of circ‐CDC45. High circ‐CDC45 expression was found in GM specimens and cells, which was tightly related to a larger tumor size, higher world health organization (WHO) stages, and worse survival for patients with GM. Functionally, manipulation of circ‐CDC45 expression strongly affected cell growth, apoptosis, migration and invasion, which suggests the oncogenic function of circ‐CDC45 in GM oncogenesis. Stepwise mechanism studies indicated that circ‐CDC45 sponged and regulated the expression of miR‐516b and miR‐527 to promote cell growth and invasion. Briefly, the regulatory network of circ‐CDC45/miR‐516b/miR‐527 plays a pivotal role in GM tumorigenesis and may act as a potential target for GM treatment.

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Downregulated circular RNA hsa_circ_0001649 regulates proliferation, migration and invasion in cholangiocarcinoma cells

Cited by 155 publications

References 26 publications

Increased Expression of Circular RNA circ_0005230 Indicates Dismal Prognosis in Breast Cancer and Regulates Cell Proliferation and Invasion via miR-618/ CBX8 Signal Pathway

Increased Expression of Circular RNA circ_0005230 Indicates Dismal Prognosis in Breast Cancer and Regulates Cell Proliferation and Invasion via miR-618/ CBX8 Signal Pathway

Circular RNA circ_0079593 indicates a poor prognosis and facilitates cell growth and invasion by sponging miR‐182 and miR‐433 in glioma

Overexpression of circular RNA circ‐CDC45 facilitates glioma cell progression by sponging miR‐516b and miR‐527 and predicts an adverse prognosis

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