Downregulated expression of S2-RNase attenuates self-incompatibility in “Guiyou No. 1” pummelo

Hu, Jianbing; Xu, Qiang; Liu, Chenchen; Liu, Binghao; Deng, Chong-Ling; Chen, Chuanwu; Wei, Zhuangmin; Ahmad, Muhammad Husnain; Peng, Kang; Wen, Hao; Xiang-ling, Chen; Chen, Peng; Larkin, Robert M.; Ye, Junli; Deng, Xiuxin; Chai, Lijun

doi:10.1038/s41438-021-00634-8

Cited by 18 publications

(19 citation statements)

References 60 publications

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“…To evaluate heterozygosity within genomes and the association of heterozygosity with the reproductive modes, we estimated genic hemizygosity based on presence and absence variants (PAVs) in five assemblies with deep long-read coverage (>100-fold). The five assemblies included: a doubled-haploid sweet orange, which served as homozygous control; our inbred Hongkong kumquat; a diploid pummelo cultivar, ‘GY’, that was sexually reproducing [ 39 ], an apomictic diploid sweet orange cultivar, ‘UKXC’ [ 38 ], and an apomictic diploid wild trifoliate orange, ‘ZK8’ (Fig. 1 c and Table S6) [ 7 ].…”

Section: Resultsmentioning

confidence: 99%

Structural variation and parallel evolution of apomixis in citrus during domestication and diversification

Wang

Song

et al. 2022

National Science Review

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Apomixis, or asexual seed formation is prevalent in the Citrinae via a mechanism termed nucellar or adventitious embryony. Here, multiple embryos of a maternal genotype form directly from nucellar cells in the ovule and can outcompete the developing zygotic embryo as they utilize the sexually derived endosperm for growth. Whilst nucellar embryony enables the propagation of clonal plants of maternal genetic constitution, it is also a barrier to effective breeding through hybridization. To address the genetics and evolution of apomixis in the Citrinae, a chromosome-level genome of Hongkong kumquat (Fortunella hindsii) was assembled following a genome-wide variation map including structural variants (SVs) based on 234 Citrinae accessions. This map revealed that hybrid citrus cultivars shelter genome-wide deleterious mutations and SVs into heterozygous states free from recessive selection, which may explain the capability of nucellar embryony in most cultivars during Citrinae diversification. Analyses revealed that parallel evolution may explain the repeated origin of apomixis in different genera of Citrinae. Within Fortunella, we found that apomixis of some varieties originated via introgression. In apomictic Fortunella, the locus associated with apomixis contains the FhRWP gene, encoding an RWP-RK domain-containing protein previously shown to be required for nucellar embryogenesis in Citrus. We found the heterozygous SV in the FhRWP and CitRWP promoters from apomictic Citrus or Fortunella due to either two or three Miniature inverted transposon element (MITE) insertions. A transcription factor FhARID, encoding an AT-rich interaction domain-containing protein binds to the MITEs in the promoter of apomictic varieties which facilitates induction of nucellar embryogenesis. This study provides evolutionary genomic and molecular insights into apomixis in Citrinae and has potential ramifications for citrus breeding.

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Section: Resultsmentioning

confidence: 99%

Structural variation and parallel evolution of apomixis in citrus during domestication and diversification

Wang

Song

et al. 2022

National Science Review

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“…The mutation of S-RNase has been found to be related to the transition from SI to SC. For example, the loss of SI in “Guiyou” pummelo ( C. maxima , Rutaceae) was attributed to a pistil-side Sm-RNase mutation ( Liang et al 2020 ; Hu et al 2021 ). Moreover, the production of a stable SC phenotype was caused by a loss-of-function mutation in some Solanaceae species ( Solanum pennellii, Solanum habrochaites , and Solanum arcanum ) ( Kubo et al 2015 ; Ma et al 2021 ).…”

Section: Discussionmentioning

confidence: 99%

The Origin and Evolution of RNase T2 Family and Gametophytic Self-incompatibility System in Plants

Qiao

Zhang

et al. 2022

Genome Biology and Evolution

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Ribonuclease (RNase) T2 genes are found widely in both eukaryotes and prokaryotes, and genes from this family have been revealed to have various functions in plants. In particular, S-RNase is known to be the female determinant in the S-RNase-based gametophytic self-incompatibility system. However, the origin and evolution of the RNase T2 gene family and gametophytic self-incompatibility system are not well understood. In this study, 785 RNase T2 genes were identified in 81 sequenced plant genomes representing broad-scale diversity, and divided into three subgroups (Class I, II, and III) based on phylogenetic and synteny network analysis. Class I was found to be of ancient origin and to emerge in green algae, Class II was shown to originate with the appearance of angiosperms, while Class III was discovered to be eudicot-specific. Each of three major classes could be further classified into several subclasses of which some subclasses were found to be lineage-specific. Furthermore, duplication, deletion, or inactivation of the S/S-like-locus was revealed to be linked to repeated loss and gain of self-incompatibility in different species from distantly related plant families with gametophytic self-incompatibility. Finally, the origin and evolutionary history of S-locus in Rosaceae species was unraveled with independent loss and gain of S-RNase occurred in different subfamilies of Rosaceae. Our findings provide insights into the origin and evolution of the RNase T2 family and the gametophytic self-incompatibility system in plants.

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“…The Chinese cultivars 'Guiyou No. 1' and 'Zigui Shatian' are self-compatible mutants of selfincompatible 'Shatian' pummelo (Chai et al, 2010b;Hu et al, 2021). A contrasting example of the development of self-compatibility from a self-incompatible cultivar has been reported in 'Wuzishatangju', which is self-incompatible and developed from the selfcompatible 'Shatangju' (Ye et al, 2009).…”

Section: Mutants Of Citrus Varieties Regarding Selfincompatibilitymentioning

confidence: 99%

Self-incompatibility Related to Seedless Fruit Production in Citrus Plants

Honsho

2023

Hort. J.

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Self-incompatibility in Citrus species is an important trait related to fruit set and seed formation. In particular, self-incompatible citrus varieties combined with sufficient parthenocarpy produce seedless fruits. The characteristics of self-incompatibility have been studied for many years, and essential traits, such as pollen tube elongation behavior and self-incompatibility genotypes, have been characterized. Recently, it has been shown that the genetic mechanism of self-incompatibility in citrus varieties is S-RNase-based gametophytic self-incompatibility. To date, 18 S-RNases (17 self-incompatible alleles and 1 self-compatible allele) have been identified. The DNA markers for S-RNases can enable the early identification of self-incompatibility/ compatibility status. The expression of self-compatibility in Citrus species is ascribed to the presence of the self-compatibility S m allele, which is a defective S-RNase, and to the suppression of S-RNase expression. Polyploidization induces self-compatibility in Citrus species: Citrus tamurana 'Hyuganatsu' is substantially self-incompatible; however, its bud mutation, 'Nishiuchi Konatsu', is self-compatible. 'Nishiuchi Konatsu' is diploid; however, it forms unreduced pollen, which causes the breakdown of self-incompatible reaction when self-pollinated because of a competitive interaction within the same individual. In addition, after fertilization by unreduced pollen, seed formation is also inhibited by triploid block caused by interploid hybridization between diploid pollen and haploid egg cells. Therefore, 'Nishiuchi Konatsu' shows self-compatibility regardless of the self-incompatibility haplotype and produces fruits with few seeds. The seedlessness trait could be beneficial for citrus breeding in the future; however, the genetic mechanisms involved in the expression of this trait remain unclear. This review focuses on the recent advances in the genetics of self-incompatibility in citrus plants, implicating the mechanisms involved in self-incompatibility and their applications for achieving the desired trait of seedlessness in citrus fruits.

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Downregulated expression of S2-RNase attenuates self-incompatibility in “Guiyou No. 1” pummelo

Cited by 18 publications

References 60 publications

Structural variation and parallel evolution of apomixis in citrus during domestication and diversification

Structural variation and parallel evolution of apomixis in citrus during domestication and diversification

The Origin and Evolution of RNase T2 Family and Gametophytic Self-incompatibility System in Plants

Self-incompatibility Related to Seedless Fruit Production in <i>Citrus</i> Plants

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