Downregulated Expression of Integrin α6 by Transforming Growth Factor-β1 on Lens Epithelial Cells in Vitro

Lim, Jung Min; Kim, Jeong‐A; Lee, Jin-Hak; Joo, Choun‐Ki

doi:10.1006/bbrc.2001.4942

Cited by 19 publications

(3 citation statements)

References 46 publications

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“…STK4 is an important signal transducer for the TGF beta family [28]. TGF beta induced down regulation of ITGA6 and upregulation of CILP2 (cartilage intermediate layer protein 2) has already been demonstrated in other tissue models [29,30]. Therefore these genes may provide good candidates with which to test the importance of TGF beta signalling in our model and also the importance of stromal derived TGF beta.…”

Section: Discussionmentioning

confidence: 97%

Stromal upregulation of lateral epithelial adhesions: Gene expression analysis of signalling pathways in prostate epithelium

et al. 2011

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BackgroundStromal signalling increases the lateral cell adhesions of prostate epithelial cells grown in 3D culture. The aim of this study was to use microarray analysis to identify significant epithelial signalling pathways and genes in this process.MethodsMicroarray analysis was used to identify genes that were differentially expressed when epithelial cells were grown in 3D Matrigel culture with stromal co-culture compared to without stroma. Two culture models were employed: primary epithelial cells (ten samples) and an epithelial cell line (three experiments). A separate microarray analysis was performed on each model system and then compared to identify tissue-relevant genes in a cell line model.ResultsTGF beta signalling was significantly ranked for both model systems and in both models the TGF beta signalling gene SOX4 was significantly down regulated. Analysis of all differentially expressed genes to identify genes that were common to both models found several morphology related gene clusters; actin binding (DIAPH2, FHOD3, ABLIM1, TMOD4, MYH10), GTPase activator activity (BCR, MYH10), cytoskeleton (MAP2, MYH10, TMOD4, FHOD3), protein binding (ITGA6, CD44), proteinaceous extracellular matrix (NID2, CILP2), ion channel/ ion transporter activity (CACNA1C, CACNB2, KCNH2, SLC8A1, SLC39A9) and genes associated with developmental pathways (POFUT1, FZD2, HOXA5, IRX2, FGF11, SOX4, SMARCC1).ConclusionsIn 3D prostate cultures, stromal cells increase lateral epithelial cell adhesions. We show that this morphological effect is associated with gene expression changes to TGF beta signalling, cytoskeleton and anion activity.

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Section: Discussionmentioning

confidence: 97%

Stromal upregulation of lateral epithelial adhesions: Gene expression analysis of signalling pathways in prostate epithelium

et al. 2011

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“…This may require further confirmation. Previous studies have shown that TGF-β1 may downregulate total LM mRNA expression in HLE B-3 cells [60]. However, it induces aberrant deposition of LMα2, LMα1 and LMβ1 in glomerular BMs [61].…”

Section: Discussionmentioning

confidence: 99%

“…TGF-β1 was highly expressed in LECs with kinds of cataracts, such anterior subcapsular cataract [54], posterior capsular opacification [55], and diabetic cataract [56,57], and was found to increase with age [58,59]. TGF-β1 downregulated the mRNA level of total LM in apoptotic HLE B-3 cells [60], and induced aberrant deposition of LMα2-α1 and LMβ1 in glomerular BMs [61]. It also may stimulate the expression of type I collagen in bovine lens epithelial explants and intact rabbit lens [62].…”

Section: Introductionmentioning

confidence: 99%

Laminin α4 overexpression in the anterior lens capsule may contribute to the senescence of human lens epithelial cells in age-related cataract

Sun

et al. 2019

Aging

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Senescence is a leading cause of age-related cataract (ARC). The current study indicated that the senescence-associated protein, p53, total laminin (LM), LMα4, and transforming growth factor-beta1 (TGF-β1) in the cataractous anterior lens capsules (ALCs) increase with the grades of ARC. In cataractous ALCs, patient age, total LM, LMα4, TGF-β1, were all positively correlated with p53. In lens epithelial cell (HLE B-3) senescence models, matrix metalloproteinase-9 (MMP-9) alleviated senescence by decreasing the expression of total LM and LMα4; TGF-β1 induced senescence by increasing the expression of total LM and LMα4. Furthermore, MMP-9 silencing increased p-p38 and LMα4 expression; anti-LMα4 globular domain antibody alleviated senescence by decreasing the expression of p-p38 and LMα4; pharmacological inhibition of p38 MAPK signaling alleviated senescence by decreasing the expression of LMα4. Finally, in cataractous ALCs, positive correlations were found between LMα4 and total LM, as well as between LMα4 and TGF-β1. Taken together, our results implied that the elevated LMα4, which was possibly caused by the decreased MMP-9, increased TGF-β1 and activated p38 MAPK signaling during senescence, leading to the development of ARC. LMα4 and its regulatory factors show potential as targets for drug development for prevention and treatment of ARC.

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Inhibitory Effects of Salmosin, a Disintegrin, on Posterior Capsular Opacification in vitro and in vivo

Kim

Lee

Chung

et al. 2002

Experimental Eye Research

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Downregulated Expression of Integrin α6 by Transforming Growth Factor-β1 on Lens Epithelial Cells in Vitro

Cited by 19 publications

References 46 publications

Stromal upregulation of lateral epithelial adhesions: Gene expression analysis of signalling pathways in prostate epithelium

Stromal upregulation of lateral epithelial adhesions: Gene expression analysis of signalling pathways in prostate epithelium

Laminin α4 overexpression in the anterior lens capsule may contribute to the senescence of human lens epithelial cells in age-related cataract

Inhibitory Effects of Salmosin, a Disintegrin, on Posterior Capsular Opacification in vitro and in vivo

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