Downregulated gelsolin expression in hyperplastic and neoplastic lesions of the prostate

Lee, Hsiang-Kuang; Driscoll, Deborah L.; Asch, Harold L.; Asch, Bonnie B.; Zhang, Paul J.

doi:10.1002/(sici)1097-0045(19990615)40:1<14::aid-pros2>3.0.co;2-6

Cited by 70 publications

(49 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This was consistent with previous observations that gelsolin was down-regulated in several types of transformed cells and tumors (23)(24)(25). The partial or total loss of gelsolin expression in a majority of breast cancers of diverse aetiologies has led to the proposal that gelsolin is a candidate tumor suppressor gene for breast cancer (26).…”

Section: Description Of Differentially Expressed Proteinssupporting

confidence: 94%

Differential Proteome Analysis of Replicative Senescence in Rat Embryo Fibroblasts

Benvenuti

Cramer

Quinn

et al. 2002

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

Normal somatic cells undergo a finite number of divisions and then cease dividing whereas cancer cells are able to proliferate indefinitely. To identify the underlying mechanisms that limit the mitotic potential, a two-dimensional differential proteome analysis of replicative senescence in serially passaged rat embryo fibroblasts was undertaken. Triplicate independent two-dimensional gels containing over 1200 spots each were run, curated, and analyzed. This revealed 49 spots whose expression was altered more than 2-fold. Of these, 42 spots yielded positive protein identification by mass spectrometry comprising a variety of cytoskeletal, heat shock, and metabolic proteins, as well as proteins involved in trafficking, differentiation, and protein synthesis, turnover, and modification. These included gelsolin, a candidate tumor suppressor for breast cancer, and ␣-glucosidase II, a member of the family of glucosidases that includes klotho; a defect in klotho expression in mice results in a syndrome that resembles human aging. Changes in expression of TUC-1, -2, -4, and -4␤, members of the TUC family critical for neuronal differentiation, were also identified. Some of the identified changes were also shown to occur in two other models of senescence, premature senescence of REF52 cells and replicative senescence of mouse embryo fibroblasts. The majority of these candidate proteins were unrecognized previously in replicative senescence.

show abstract

Section: Description Of Differentially Expressed Proteinssupporting

confidence: 94%

Differential Proteome Analysis of Replicative Senescence in Rat Embryo Fibroblasts

Benvenuti

Cramer

Quinn

et al. 2002

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

show abstract

“…Unlike CapG, gelsolin expression has been studied in a variety of cancers, including bladder (Tanaka et al, 1995), lung (Dosaka-Akita et al, 1998), prostate (Lee et al, 1999), and cervical cancer (Liao et al, 2011). Gelsolin has been reported to be down-regulated in a subset of NSCLC in some studies (Dosaka-Akita et al, 1998), whereas other studies reported that it was up-regulated in NSCLC (Yang et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Prognostic Evaluation of CapG, Gelsolin, P‐gp, GSTP1, and Topo‐II Proteins in Non‐Small Cell Lung Cancer

Zhu

Hunag

Liu

et al. 2011

The Anatomical Record

View full text Add to dashboard Cite

Metastasis and multidrug resistance (MDR) are the main reasons for the poor prognosis of non-small cell lung cancer (NSCLC) patients. The use of biomarkers may contribute to a more accurate prediction of tumor metastasis, a better response to chemotherapy, and better patient survival. Gelsolin-like actin-capping protein (CapG) and gelsolin have been identified as playing important roles in tumor invasion and metastasis. Permeability glycoprotein (P-gp), glutathione S-transferase pi (GSTP1), and topoisomerase-II (Topo-II) are proteins that are closely related to MDR. In this study, we assessed the prognostic significance of CapG and gelsolin (both markers of tumor motility), and of P-gp, GSTP1, and Topo-II (markers of MDR) in NSCLC patients. One hundred and twenty-one patients with pathologically confirmed, resectable NSCLC were included in the study. The expression levels of the five kinds of proteins mentioned above were determined by immunohistochemistry (IHC). The correlation between the clinical characteristics and IHC findings were analyzed. Expression of CapG, gelsolin, and P-gp was found to be associated with an increased risk of death (Hazard Ratio (HR) ¼ 2.799, 95% Confidence Interval (CI) ¼ 1.2705-6.169, P ¼ 0.011; HR ¼ 3.968, 95% CI ¼ 1.811-8.693, P ¼ 0.001; HR ¼ 3.251, 95% CI ¼ 1.456-7.260, P ¼ 0.004, respectively), whereas expression of GSTP1 and Topo-II was not. These results suggest that higher tumor motility and MDR may be important in NSCLC prognosis. Anat Rec, 295:208-214, 2012. V V C 2011 Wiley Periodicals, Inc.Key words: NSCLC; CapG; gelsolin; P-gp; prognosisLung cancer remains the leading cause of cancerrelated deaths in China, in both males and females. The most common form of lung cancer, non-small cell lung cancer (NSCLC), has a 5-year relative survival rate below 70% in patients with Stage I tumors, and below 1% in patients with Stage IV tumors (Jemal et al., 2009). Treatment of patients with NSCLC is governed by various prognostic factors such as surgical stage, histological grade, differentiation, and lymph node metastasis. Nevertheless, the clinical outcome in individual patients is often difficult to predict.The main cause of death from lung cancer is usually related to metastasis and resistance to chemotherapeutics after primary surgery. If prognosis could be determined more precisely before treatment, then patients would be more effectively treated with individualized therapy. Compared with mRNAs, which degrade quickly if samples are not prepared correctly, proteins are more stable and potentially very useful biomarkers. Furthermore, differences in protein expression may reveal information about biological properties of tumors.

show abstract

“…Several reports showed that gelsolin expression was low in the poorly differentiated stages of bladder cancer, 24 ovarian cancer, 25 and colon cancer. 26 Lee et al 27 examined gelsolin expression in 72 prostatectomy specimens with adenocarcinoma and 8 nonneoplastic prostates from autopsies. The expression was decreased in prostatic adenocarcinoma in comparison with nonproliferative tissue.…”

Section: Gelsolin-mediated Invasion Of Prostate Cancer K Hashimoto Et Almentioning

confidence: 99%

The potential of neurotensin secreted from neuroendocrine tumor cells to promote gelsolin-mediated invasiveness of prostate adenocarcinoma cells

Hashimoto

Kyoda

Tanaka

et al. 2015

Laboratory Investigation

View full text Add to dashboard Cite

Neuroendocrine (NE) cells in prostate cancer have been shown to be associated with the progression of prostate cancer. However, little is known about the molecular basis of this association. We have previously demonstrated that NE cells promote metastasis of a human prostate cancer cell line (LNCaP) with overexpression of the gelsolin gene. The purpose of this study was to investigate the interactions between NE cells and LNCaP cells and the involvement of gelsolin in contributing to the invasive potential of LNCaP cells. In addition, we examined whether neurotensin induced gelsolin-mediated invasion. We used the NE cell line NE-CS that was established from the prostate of the LPB-Tag 12T-10 transgenic mouse. Small interfering RNA (siRNA) targeting gelsolin or not targeting it was transfected into LNCaP cells.

show abstract

Downregulated gelsolin expression in hyperplastic and neoplastic lesions of the prostate

Cited by 70 publications

References 19 publications

Differential Proteome Analysis of Replicative Senescence in Rat Embryo Fibroblasts

Differential Proteome Analysis of Replicative Senescence in Rat Embryo Fibroblasts

Prognostic Evaluation of CapG, Gelsolin, P‐gp, GSTP1, and Topo‐II Proteins in Non‐Small Cell Lung Cancer

The potential of neurotensin secreted from neuroendocrine tumor cells to promote gelsolin-mediated invasiveness of prostate adenocarcinoma cells

Contact Info

Product

Resources

About