Downregulation of CDC20 suppressed cell proliferation, induced apoptosis, triggered cell cycle arrest in osteosarcoma cells, and enhanced chemosensitivity to cisplatin

Abstract

Osteosarcoma (OS) is a common malignant bone tumor that occurs in adolescents or children under the age of 20, which is extremely difficult to cure and has a high recurrence rate. Recent studies showed that cell division cycle 20 (CDC20) overexpression is associated wit h poor prognosis in patients with osteosarcoma. However, the function of CDC20 in osteosarcoma has not been investigated clearly. In this study, we aim to explore the role of CDC20 in two independent human py sensitivity. We applied multiple approaches to measure cell growth, cell cycle, and apoptosis with or without deregulation or overexpression of CDC20. We found that the downregulation of CDC20 by siRNA or apcin suppressed cell proliferation, induced apoptosis, and triggered cell cycle arrest. Consistently, overexpression of CDC20 in normal cells promoted cell growth, inhibited apoptosis. What's more, the additional treatment of siCDC20 or apcin achieved better anticancer effects than that of cisplatin alone. Furthermore, Bim and p21 were upregulated in OS cells following apcin treatment. Altogether, the results of the present study demonstrated that targeting CDC20 could be useful for the treatment of OS, and might be a promising solution for the treatment of the OS with cisplatin insensitivity.

Dynamic transcriptome and chromatin architecture in granulosa cells during chicken folliculogenesis

“…Our results demonstrated that candidate loci can be analyzed in future studies of folliculogenesis. Typically, CCNA1 59 , CDC20 60 , and CPEB1 61 (which are cell cycle regulators) and BRDT 62 and FBXO43 63 (which are involved in cell meiosis) showed more PEIs and contacted with SEs during the SWF stage, though these interactions and active enhancers decreased in later stages (Supplementary Fig. 12a–e ).…”

Gene Expression Profiles Analyzed Using Integrating RNA Sequencing, and Microarray Reveals Increased Inflammatory Response, Proliferation, and Osteoclastogenesis in Pigmented Villonodular Synovitis

“…The tumor-related phenotypes observed locally in PVNS tissues mainly include increased cell proliferation and migration. Cell division cycle protein 20 ( 26 ), cyclin-dependent kinase subunit 2 ( 27 ), and E2F transcription factor 2 ( 28 ) were reported to be closely associated with cell proliferation and cell cycle. In addition, coactosin-like F-actin binding protein 1 ( 29 ), Rac family small GTPase 2, and RAC1 guanine nucleotide exchange factor mediated cytoskeleton assembly and chemotaxis ( 30 ).…”

CDC20 in and out of mitosis: a prognostic factor and therapeutic target in hematological malignancies

“…Recently, there is different evidence which suggest that CDC20 contributes to a number of cellular processes that have been partially explored. An overexpression and/or oncogenic role of CDC20 has been already described in a variety of human solid tumors [ 9 , 10 ] including pancreas [ 11 , 12 ], breast [ 13 , 14 ], lung [ 15 , 16 ], prostate [ 17 – 19 ], gastric, colorectal [ 16 , 18 ], hepatocellular [ 20 , 21 ], kidney [ 22 ], ovarian cancer [ 23 , 24 ], osteosarcoma [ 25 – 27 ] and glioblastoma [ 28 , 29 ]. Moreover, CDC20 expression level has been reported as a putative marker of clinical outcome in many cancer types, being associated with advanced stage, high grade and poor prognosis [ 9 , 10 , 30 , 31 ].…”