Downregulation of eukaryotic translation initiation factor 3b inhibited proliferation and metastasis of gastric cancer

Ma, Fengwang; Xue, Li; Ren, Juchao; Guo, Ruiting; Li, Yuwei; Liu, Jichang; Sun, Yuan; Liu, Zhifang; Jia, Jihui; Li, Wenjuan

doi:10.1038/s41419-019-1846-0

Cited by 24 publications

(23 citation statements)

References 34 publications

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“…All of these results indicate that EIF3B has an important role in cancer-associated processes. A previous study suggested that EIF3B is involved in the proliferation and metastasis of gastric cancer (39). In addition, the present study further determined that EIF3B is associated with the histologic grade and survival status of patients with liver cancer.…”

Section: Discussionsupporting

confidence: 72%

Expression of eukaryotic translation initiation factor 3 subunit B in liver cancer and its prognostic significance

et al. 2020

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Liver cancer is one of the major malignancies with the worst prognosis among all solid tumor types. It is therefore ponderable to explore prognostic biomarkers and therapeutic targets for liver cancer. Eukaryotic translation initiation factor 3 subunit B (EIF3B) is closely linked to the transcription initiation of cancer-associated genes. In the present study, EIF3B was indicated to be a potential prognostic biomarker of liver cancer. The mRNA expression level of EIF3B in liver cancer was assessed by analyzing the Cancer Genome Atlas dataset. χ 2 and Fisher's exact tests were used to assess the association of EIF3B expression with clinical parameters. Receiver-operating characteristic curve analysis was used for evaluating the diagnostic value of EIF3B. Overall and relapse-free survival were assessed using Kaplan-Meier curves to determine the association between EIF3B expression and survival. Univariate and multivariate Cox regression analysis were performed to identify the factors affecting overall/relapse-free survival. Gene set enrichment analysis (GSEA) was used to identify signaling pathways associated with EIF3B in liver cancer. It was revealed that EIF3B was highly expressed in liver cancer tissues and it had a promising diagnostic ability. Furthermore, the survival analysis indicated that patients with high EIF3B expression generally had shorter overall as well as relapse-free survival. Univariate and multivariate Cox analysis suggested that high EIF3B mRNA expression may serve as an independent biomarker for the prognostication of patients with liver cancer. GSEA suggested that MYC-V1 (HALLMARK_MYC_TARGETS_V1 geneset; P=0.009), MYC-V2 (HALLMARK_MYC_TARGETS_V2 geneset; P=0.004) and DNA repair pathways (HALLMARK_ DNA_REPAIR geneset; P<0.001) were differentially enriched in high EIF3B expression and low EIF3B expression groups. In conclusion, high EIF3B expression was indicated to be an independent prognostic biomarker for patients with liver cancer.

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Section: Discussionsupporting

confidence: 72%

Expression of eukaryotic translation initiation factor 3 subunit B in liver cancer and its prognostic significance

et al. 2020

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“…For example, reports revealed DDX21 could affect the proliferation of GC cells by up‐regulating levels of cyclin D1 and CDK2 . Likewise, the suppression of EIF3B inhibits the proliferation and metastasis of GC by effectively modulating the expression of cancer‐related genes . Likewise, we also found 15 co‐expression relationships in RBP interactions such as CPSF3 , DDX18 , DKC1 , and FUS (Table ).…”

Section: Resultssupporting

confidence: 58%

Function and regulation annotation of up‐regulated long non‐coding RNA LINC01234 in gastric cancer

Zhu

Luo

Korakkandan

et al. 2020

Clinical Laboratory Analysis

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Background Accumulated evidences indicate that long non‐coding RNAs (lncRNAs) participate in many biological mechanisms. Moreover, it acts as an essential regulator in various human diseases such as gastric cancer (GC). Nevertheless, the comprehensive regulatory roles and clinical significance of most lncRNAs in GC are not fully understood. Methods In this research, our aim was to investigate the underlying mechanism of lncRNA LINC01234 in GC. Firstly, the usage of qRT‐PCR helped to establish expression pattern of LINC01234 in GC tissues. Following this, appropriate statistical tests were applied to analyze the relation between expression level and clinicopathological factors. Ultimately, potential functions and regulatory network of LINC01234 were concluded via GSEA and a series of bioinformatics tools or databases, respectively. Results Consequently, at the end of research we found LINC01234 is up‐regulated in GC tissues in comparison with adjacent normal tissues. Furthermore, its expression level is correlated with differentiation of patients with GC. It is also important to highlight bioinformatics analysis revealed that LINC01234 is involved in cancer‐associated pathways such as cell cycle and mismatch repair. Also, regulatory network of LINC01234 presented a probability in the involvement of tumorigenesis through regulating cancer‐associated genes. Conclusion Overall, our results suggested that LINC01234 may play a crucial role in GC.

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“…Protein extraction and western blot were performed as previously described [28]. Antibodies were as follows: NFIB (Abcam, ab186738, Cambridge, UK, 1:1000 dilution) and GAPDH (CST, #5174, MA, USA, 1:3000 dilution).…”

Section: Protein Extraction and Western Blotmentioning

confidence: 99%

Hsa_circ_0026416 promotes proliferation and migration in colorectal cancer via miR-346/NFIB axis

Liang

Shi

et al. 2020

Cancer Cell Int

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Background Colorectal cancer (CRC) is one of the most common cancers worldwide. Circular RNAs (circRNAs), a novel class of non-coding RNAs, have been confirmed to be key regulators of many diseases. With many scholars devoted to studying the biological function and mechanism of circRNAs, their mysterious veil is gradually being revealed. In our research, we explored a new circRNA, hsa_circ_0026416, which was identified as upregulated in CRC with the largest fold change (logFC = 3.70) of the evaluated circRNAs via analysing expression profiling data by high throughput sequencing of members of the GEO dataset (GSE77661) to explore the molecular mechanisms of CRC. Methods qRT-PCR and western blot analysis were utilized to assess the expression of hsa_circ_0026416, miR-346 and Nuclear Factor I/B (NFIB). CCK-8 and transwell assays were utilized to examine cell proliferation, migration and invasion in vitro, respectively. A luciferase reporter assay was used to verify the combination of hsa_circ_0026416, miR-346 and NFIB. A nude mouse xenograft model was also utilized to determine the role of hsa_circ_0026416 in CRC cell growth in vivo. Results Hsa_circ_0026416 was markedly upregulated in CRC patient tissues and plasma and was a poor prognosis in CRC patients. In addition, the area under the curve (AUC) of hsa_circ_0026416 (0.767) was greater than the AUC of CEA (0.670), CA19-9 (0.592) and CA72-4 (0.575). Functionally, hsa_circ_0026416 promotes cell proliferation, migration and invasion both in vitro and in vivo. Mechanistically, hsa_circ_0026416 may function as a ceRNA via competitively absorbing miR-346 to upregulate the expression of NFIB. Conclusions In summary, our findings demonstrate that hsa_circ_0026416 is an oncogene in CRC. Hsa_circ_0026416 promotes the progression of CRC via the miR-346/NFIB axis and may represent a potential biomarker for diagnosis and therapy in CRC.

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Downregulation of eukaryotic translation initiation factor 3b inhibited proliferation and metastasis of gastric cancer

Cited by 24 publications

References 34 publications

Expression of eukaryotic translation initiation factor 3 subunit B in liver cancer and its prognostic significance

Expression of eukaryotic translation initiation factor 3 subunit B in liver cancer and its prognostic significance

Function and regulation annotation of up‐regulated long non‐coding RNA LINC01234 in gastric cancer

Hsa_circ_0026416 promotes proliferation and migration in colorectal cancer via miR-346/NFIB axis

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