Downregulation of gene expression and activity of GRIM-19 affects mouse oocyte viability, maturation, embryo development and implantation

Lan, Chao; Xiao, Wang; Yang, Yang; Wen-juan, Cui; Xu, Jing; Chen, Honglei; Hao, Aijun; Deng, Xiaohui

doi:10.1007/s10815-014-0413-y

Cited by 7 publications

(4 citation statements)

References 37 publications

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“…Negative: No fluorescence control; Positive: Embryos pretreated with DNAse to ensure TUNEL staining was successful; 5 embryos in each group found in our study, we might presume that the blastocysts derived from heifers after they reach puberty have considerably higher developmental competence, caused by more efficient respiratory chain action. To support the hypothesis that lower expression of NDUFA13 found in the blastocysts derived from oocytes collected from prepubertal heifers accounts for the probable higher lethality of those embryos, expressed by lower blastocyst rate in that group of blastocysts compared to the group from pubertal heifers, we would like to cite the studies of Huang et al [45], Chao et al [46] and Cui et al [47] in mice. In those experiments, it was shown previously that the downregulation of the NDUFA13 gene caused a reduction in mouse oocyte viability, maturation performance, and the developmental and implantation ability of embryos.…”

Section: Discussionmentioning

confidence: 91%

Deregulation of oxidative phosphorylation pathways in embryos derived in vitro from prepubertal and pubertal heifers based on whole-transcriptome sequencing

Traut,

Kowalczyk-Zieba,

Boruszewska

et al. 2024

BMC Genomics

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Background Although, oocytes from prepubertal donors are known to be less developmentally competent than those from adult donors it does not restrain their ability to produce full-term pregnancies. The transcriptomic profile of embryos could be used as a predictor for embryo’s individual developmental competence. The aim of the study was to compare transcriptomic profile of blastocysts derived from prepubertal and pubertal heifers oocytes. Bovine cumulus-oocyte complexes (COCs) were obtained by ovum pick- up method from prepubertal and pubertal heifers. After in vitro maturation COCs were fertilized and cultured to the blastocyst stage. Total RNA was isolated from both groups of blastocysts and RNA-seq was performed. Gene ontology analysis was performed by DAVID (Database for Annotation, Visualization and Integrated Discovery). Results A higher average blastocyst rate was obtained in the pubertal than in the pre-pubertal group. There were no differences in the quality of blastocysts between the examined groups. We identified 436 differentially expressed genes (DEGs) between blastocysts derived from researched groups, of which 247 DEGs were downregulated in blastocysts derived from pubertal compared to prepubertal heifers oocytes, and 189 DEGs were upregulated. The genes involved in mitochondrial function, including oxidative phosphorylation (OXPHOS) were found to be different in studied groups using Kyoto Encyclopedia of Genes (KEGG) pathway analysis and 8 of those DEGs were upregulated and 1 was downregulated in blastocysts derived from pubertal compared to prepubertal heifers oocytes. DEGs associated with mitochondrial function were found: ATP synthases (ATP5MF-ATP synthase membrane subunit f, ATP5PD- ATP synthase peripheral stalk subunit d, ATP12A- ATPase H+/K + transporting non-gastric alpha2 subunit), NADH dehydrogenases (NDUFS3- NADH: ubiquinone oxidoreductase subunit core subunit S3, NDUFA13- NADH: ubiquinone oxidoreductase subunit A13, NDUFA3- NADH: ubiquinone oxidoreductase subunit A3), cytochrome c oxidase (COX17), cytochrome c somatic (CYCS) and ubiquinol cytochrome c reductase core protein 1 (UQCRC1). We found lower number of apoptotic cells in blastocysts derived from oocytes collected from prepubertal than those obtained from pubertal donors. Conclusions Despite decreased expression of genes associated with OXPHOS pathway in blastocysts from prepubertal heifers oocytes, the increased level of ATP12A together with the lower number of apoptotic cells in these blastocysts might support their survival after transfer.

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Section: Discussionmentioning

confidence: 91%

Deregulation of oxidative phosphorylation pathways in embryos derived in vitro from prepubertal and pubertal heifers based on whole-transcriptome sequencing

Traut,

Kowalczyk-Zieba,

Boruszewska

et al. 2024

BMC Genomics

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“…Previous studies have shown a lack of NDUFA13 leads to the abnormality of the mitochondrion structure, morphology and cellular distribution (Chao et al, 2015;Huang et al, 2004). A deficiency of NDUFA13 leads to a collapse of MMP and an increased apoptosis in HTR-8/SVneo cell line (Chen et al, 2015).…”

Section: Discussionmentioning

confidence: 94%

Expression of NDUFA13 in asthenozoospermia and possible pathogenesis

Yang

Cheng

Wang

et al. 2017

Reproductive BioMedicine Online

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“…The results of the current study indicated that esophageal tumor cells exhibited reduced Grim-19 mRNA and protein expression compared with normal esophageal epithelial cells. Grim-19 has been associated with reactive oxygen species induced by an IFN/RA pathway and has a conserved evolutionary history in eukaryotic cells (41,42).…”

Section: Discussionmentioning

confidence: 99%

Grim-19 expressed by recombinant adenovirus for esophageal neoplasmtarget therapy

et al. 2018

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Esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (EA) are the two most common types of esophageal cancer, which is the sixth highest cause of cancer‑associated mortality and the eighth most common cancer worldwide. Gene associated with retinoid‑interferon (IFN)‑induced mortality‑19 (Grim‑19) is reported to be a cell death activator that may be used to define mechanisms involved in IFN‑β‑ and retinoic acid‑induced cell death and apoptosis in a number of tumor cell lines. The present study constructed a recombinant adenovirus expressing Grim‑19 (rAd‑Grim‑19) and investigated its therapeutic outcomes in ESCC cells and tumor‑bearing mice. Grim‑19 expression was detected in EC‑109 (ESCC) cells by reverse transcription‑quantitative polymerase chain reaction and western blot analysis. Tumor cell death and apoptosis induced by rAd‑Grim‑19 in EC‑109 cells were analyzed by flow cytometry. The inhibitory effects of rAd‑Grim‑19 on EC‑109 growth were determined by MTT assays. Furthermore, the therapeutic effects of rAd‑Grim‑19 were investigated in EC‑109‑bearing mice. The results demonstrated that Grim‑19 mRNA and protein expression was downregulated in EC‑109 esophageal carcinoma cells compared with Het‑1A normal esophageal epithelial cells. In addition, EC‑109 cells exhibited a significant reduction in tumor cell growth in the rAd‑Grim‑19 group compared with the control groups. Furthermore, rAd‑Grim‑19 increased EC‑109 cell apoptosis compared with the control group. These results indicated that rAd-Grim-19 may regulate tumor cell growth and apoptosis. Additionally, the results demonstrated that rAd‑Grim‑19 led to beneficial outcomes and prolonged the survival of esophageal tumor‑bearing mice. In conclusion, the present study demonstrated that rAd‑Grim‑19 may have potential as an antitumor agent for esophageal neoplasms and may therefore be beneficial for patients with esophageal neoplasms.

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Downregulation of gene expression and activity of GRIM-19 affects mouse oocyte viability, maturation, embryo development and implantation

Cited by 7 publications

References 37 publications

Deregulation of oxidative phosphorylation pathways in embryos derived in vitro from prepubertal and pubertal heifers based on whole-transcriptome sequencing

Deregulation of oxidative phosphorylation pathways in embryos derived in vitro from prepubertal and pubertal heifers based on whole-transcriptome sequencing

Expression of NDUFA13 in asthenozoospermia and possible pathogenesis

Grim-19 expressed by recombinant adenovirus for esophageal neoplasmtarget therapy

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