Downregulation of high mobility group box 1 enhances the radiosensitivity of non‑small cell lung cancer by acting as a crucial target of microRNA‑107

Bai, Linquan; Zhang, Jingjing; Gao, Dongqi; Liu, Cheng-Yi; Li, Wenxin; Liu, Qingshan

doi:10.3892/etm.2021.10111

Cited by 3 publications

(4 citation statements)

References 41 publications

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Section: Discussionmentioning

confidence: 99%

“…24,25 For example, miR-129-5p and miR-1179 attenuated tumor proliferation via targeting HMGB1 in GC cells 26,27 miR-29b induced prostate cancer apoptosis by increasing Bim expression, 28 and miR-107 enhanced the radiosensitivity of nonsmall cell lung cancer by downregulating expression of HMGB1. 29 Extrapolating the results of the above research, we first analyzed the distribution of miRNAs in GC and adjacent normal tissues using the TCGA and TargetScan database, and then screened the differentially expressed miRNAs. We then predicted the identity of the miRNAs that possibly bind to the 3 ′ -UTRs of the HMGB1 gene using the TargetScan database (Table 1).…”

Section: Discussionmentioning

confidence: 99%

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Aloin Induces Gastric Cancer Cell Apoptosis via the miR-5683/HMGB1 Signal Axis

Chen

et al. 2023

Natural Product Communications

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Background: Recent studies conducted by us indicate that aloin (ALO) could promote gastric cancer (GC) cell apoptosis, but the underlying mechanism remains unclear. The high mobility group box 1 (HMGB1) has been reported to regulate the apoptosis, proliferation, and migration of several cancer types. Bioinformatics analysis suggests a possible targeted regulatory relationship between miR5683 and HMGB1. Purpose: Herein, we aimed to investigate the possible role of the miR-5683/HMGB1 axis in ALO-induced GC cell apoptosis. Methods: The expression levels of miR-5683 and HMGB1 in GC tissue and adjacent normal tissue were investigated using bioinformatics analysis, and their expressions at the cellular levels were determined using reverse transcription quantitative real-time polymerase chain reaction and western blotting (WB) assays. The interaction between miR-5683 and HMGB1 was predicted in the TargetScan database and verified by a dual-luciferase assay. Cell viability and apoptosis were assessed by the cell counting kit 8 (CCK-8) assay, 4′,6-diamidino-2-phenylindole (DAPI) staining, and flow cytometry. WB was used to examine the expression levels of apoptosis-related proteins and HMGB1. Results: Our data showed that ALO promoted GC cell apoptosis, downregulated HMGB1, upregulated miR-5683 expression, and dual-luciferase assay confirmed the interaction between HMGB1 and miR-5683. Moreover, miR-5683 mimics enhanced ALO-induced GC cell apoptosis and the inhibitory effects of ALO on the levels of HMGB1. However, miR-5683 inhibitors showed the opposite effects. Conclusion: Our data suggested that ALO promotes GC cell apoptosis through the miR-5683/HMGB1 axis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Aloin Induces Gastric Cancer Cell Apoptosis via the miR-5683/HMGB1 Signal Axis

Chen

et al. 2023

Natural Product Communications

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“…HMGB1 is a DNA-binding protein released during radiotherapy, and it is related to tumor radioresistance based on its DNA damage repair and autophagy functions [11]. HMGB1 is involved in mechanisms that regulate resistance to radiotherapy in pancreatic [13,14], cervical [8], breast [33], bladder [16,34], and lung cancers [15].…”

Section: Discussionmentioning

confidence: 99%

“…It is confirmed that knockdown of HMGB1 could decrease the proliferation and invasive ability of non-small cell lung cancer cells while also increasing the radiosensitivity of the cells [15]. Shrivastava et al showed a positive correlation between high levels of HMGB1 and radioresistance in bladder cancer cell lines.…”

Section: Introductionmentioning

confidence: 86%

HMGB1 overexpression promotes a malignant phenotype and radioresistance in ESCC

Dong¹,

Zhang²,

Du³

et al. 2022

J. Cancer

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Esophageal cancer is a common malignant disease that is generally treated with radiotherapy. High mobility group box 1 (HMGB1) plays an essential role in tumor cell proliferation, migration, and cell cycle progression. Here, we aimed to clarify the effects of HMGB1 on radioresistance in esophageal squamous cell carcinoma (ESCC) cell lines and patient survival. We performed immunohistochemistry for HMGB1 in biopsy samples of 39 stage I-III ESCC patients grouped by HMGB1 expression status. Then, 1-, 3-, 5-, and 10-year overall survival outcomes were calculated by Kaplan-Meier survival analysis. The cellular localization of HMGB1 was examined before and after irradiation by Immunofluorescence staining. Stable cell lines (KYSE30 and KYSE510) with differential HMGB1 expression were constructed using lentiviruses. Furthermore, we examined phosphorylated histone H2AX (γ-H2AX) expression in both HMGB1 overexpression and negative control groups by western blotting. HMGB1-negative expression was associated with superior ESCC patient 10-year survival (P=0.016). HMGB1 overexpression promoted cell migration, proliferation, and radioresistance and mitigated cell cycle arrest at the G0/G1 phase induced by irradiation. This demonstrates that HMGB1-positive expression is correlated with unfavorable clinical outcomes, and HMGB1 overexpression may promote the malignant phenotype of ESCC cells and induce radioresistance by regulating cell cycle distribution in ESCC.

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CircMMP11 as a prognostic biomarker mediates miR-361-3p/HMGB1 axis to accelerate malignant progression of hepatocellular carcinoma

Zou,

Zhang,

Zhu

et al. 2023

Open Medicine

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As a high metastatic tumor, patients having hepatocellular carcinoma (HCC) show poor prognosis. The carcinogenic roles of circMMP11 are generally described in the development of other cancers. However, there is a lack of studies on its involvement in HCC. Therefore, we investigated the potential role and molecular mechanisms of CircMMP11 in the development of HCC in vitro, providing preliminary evidence for the clinical treatment of HCC. First, we examined the expression of CircMMP11 in HCC tissues and cell lines in both clinical and in vitro experiments. We then used a loss-of-function assay to determine CircMMP11’s regulatory role on the malignant characteristics of HCC cells. The results showed that high expression of CircMMP11 in HCC was associated with patient overall survival. Serum CircMMP11 had good diagnostic efficacy in distinguishing HCC patients from the control group. In vitro, inhibiting CircMMP11 suppressed the malignant characteristics of human HCC cell lines by directly sequestering miR-361-3p, which further affected the downstream gene HMGB1 expression. In addition, we knocked down CircMMP11 and found that its deletion inhibited the malignant characteristics of HCC cells through the miR-361-3p/HMGB1 axis.

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Downregulation of high mobility group box 1 enhances the radiosensitivity of non‑small cell lung cancer by acting as a crucial target of microRNA‑107

Cited by 3 publications

References 41 publications

Aloin Induces Gastric Cancer Cell Apoptosis via the miR-5683/HMGB1 Signal Axis

Aloin Induces Gastric Cancer Cell Apoptosis via the miR-5683/HMGB1 Signal Axis

HMGB1 overexpression promotes a malignant phenotype and radioresistance in ESCC

CircMMP11 as a prognostic biomarker mediates miR-361-3p/HMGB1 axis to accelerate malignant progression of hepatocellular carcinoma

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