Downregulation of hsa_circ_0006220 and its correlation with clinicopathological factors in human breast cancer

Liu, Chong; Chen, Mingshi; Shi, Yue

doi:10.21037/gs-21-42

Cited by 8 publications

(8 citation statements)

References 41 publications

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“…In our previous study, hsa_circ_0006220 was identified to be downregulated in BC tissues relative to the paired noncancerous tissues using a circRNA microarray and RT-PCR (14). In the present study, hsa_circ_0006220 was shown to be more obviously downregulated in BC cells than normal breast epithelial cells, MCF-10A (see Figure 1A) by RT-PCR.…”

Section: Downregulation Of Hsa_circ_0006220 In Tnbc Tissues and Cellssupporting

confidence: 60%

“…Numerous of circRNAs have been reported to function as miRNA sponges in cancers (24,25), and circRNAs play roles in the development of tumors by their diseaseassociated target miRNAs (26). Previously, we identified 5 possible target miRNAs of hsa_circ_0006220 (14). Among them, miR-197-5p was found to be regulated by hsa_ circ_0006220.…”

Section: Discussionmentioning

confidence: 98%

“…In our previous study, hsa_circ_0006220 was found to downregulate BC cells and tissues by a circRNA microarray and real-time polymerase chain reaction (RT-PCR). Further, 5 potential target miRNAs were predicted, and hsa_circ_0006220 was confirmed to modulate miR-197-5p in BC cells (14). In addition, Shaker et al reported that the level of miR-197 was notably increased in the serum of BC patients (15).…”

Section: Introductionmentioning

confidence: 91%

“…In our previous study, we identified the 5 miRNAs most likely to be sponged by hsa_circ_0006220, and examined their level changes after the upregulation of hsa_circ_0006220 in MDA-MB-231 (14). Among the 5 miRNAs, miR-197-5p was significantly decreased when overexpressing hsa_circ_0006220; thus, it might be a target of hsa_circ_0006220.…”

Section: Hsa_circ_0006220 Acts As a Sponge Of Mir-197-5pmentioning

confidence: 99%

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CircRNA hsa_circ_0006220 acts as a tumor suppressor gene by regulating miR-197-5p/CDH19 in triple-negative breast cancer

Shi¹,

Han²,

Liu³

2021

Ann Transl Med

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Background: Triple-negative breast cancer (TNBC) is one of the most aggressive breast cancer (BC) subtypes. Circular ribonucleic acids (circRNAs) are a class of novel stable and conserved forms of ribonucleic acids (RNAs). circRNAs have been documented to be involved in multiple diseases, especially malignancies, through a competing endogenous RNA (ceRNA) mechanism. However, few studies have been conducted on the function of circRNAs in TNBC. Previously, hsa_circ_0006220 was found to be downregulated in BC tissues. The present study sought to explore the mechanism of hsa_circ_0006220 in TNBC progression. Methods: A real-time polymerase chain reaction (PCR) was used to validate the expression of hsa_ circ_0006220 in TNBC tissues and cells. In addition, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2Htetrazolium bromide (MTT), wound-healing and Transwell assays were conducted to measure the inhibition effects of hsa_circ_0006220 on TNBC cells in vitro. Further, a dual-luciferase reporter assay was performed to confirm the interaction between hsa_circ_0006220 and miR-197-5p. A mimic and an inhibitor of miR-197-5p were constructed to confirm the downstream mechanism of hsa_circ_0006220 in TNBC cells.Results: Hsa_circ_0006220 was more downregulated in TNBC than other subtypes of BC tissues and cell lines. In vitro data showed that hsa_circ_0006220 remarkably inhibited the proliferation, migration, and invasion of TNBC cells. Further, hsa_circ_0006220 was confirmed to be a sponge of miR-197-5p, and to indirectly regulate CDH19 expression. A rescue assay indicated the biological function of the hsa_ circ_0006220/miR-197-5p/CDH19 pathway in TNBC cells.Conclusions: hsa_circ_0006220 plays an inhibitory role in TNBC progression. It might be a potential diagnostic marker and therapeutic target for TNBC patients.

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Section: Downregulation Of Hsa_circ_0006220 In Tnbc Tissues and Cellssupporting

confidence: 60%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 91%

Section: Hsa_circ_0006220 Acts As a Sponge Of Mir-197-5pmentioning

confidence: 99%

See 2 more Smart Citations

CircRNA hsa_circ_0006220 acts as a tumor suppressor gene by regulating miR-197-5p/CDH19 in triple-negative breast cancer

Shi¹,

Han²,

Liu³

2021

Ann Transl Med

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“…For example, in breast cancer, circ_0006220 is reported to be downregulated in cancer tissues compared with adjacent normal tissues, 22) and the level of circ_0006220 is negatively correlated with aggressive phenotypes of cancer. 27) This phenomenon may be due to the complexity of tumorigenesis and the differences in tumor microenvironment. Compared with BEAS-2B cell line, we found that circ_0006220 level was upregulated in A-549 and NCI-H1299 cell lines.…”

Section: Discussionmentioning

confidence: 99%

Circ_0006220 Contributes to NSCLC Progression through miR-342-3p/GOT2 Axis

Tang

Zhao

et al. 2023

ATCS

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Purpose: Dysregulated circular RNAs (circRNAs) have shown crucial modulatory functions in tumorigenesis, containing non-small cell lung cancer (NSCLC). The purpose of this study was to explore the biological functions and regulatory theory of circ_0006220 in NSCLC. Methods: Reverse transcription-quantitative polymerase chain reaction and Western blot assay were conducted to measure RNA and protein expression, respectively. A total of 73 cases of NSCLC tumor samples were collected for expression analysis, and A-549 and NCI-H1299 cell lines were used for functional experiments. Cell proliferation was assessed by cell counting kit-8 assay, colony formation assay, 5-ethynyl-2’-deoxyuridine assay, and flow cytometry. Cell apoptosis, motility, and angiogenesis ability were analyzed by flow cytometry, transwell assays, and capillary-like network formation assay. Dual-luciferase reporter assay and RNA immunoprecipitation assay were conducted to verify the target relationships. Results: Circ_0006220 was highly expressed in NSCLC tissues and cell lines. Circ_0006220 silencing inhibited the proliferation, migration, invasion, and angiogenesis but induced the apoptosis of NSCLC cells. Circ_0006220 acted as a microRNA-342-3p (miR-342-3p) sponge, and circ_0006220 knockdown-induced changes on the phenotypes of NSCLC cells were largely overturned by the knockdown of miR-342-3p. miR-342-3p interacted with the 3’ untranslated region of glutamic-oxaloacetic transaminase 2 (GOT2), and GOT2 overexpression largely diminished miR-342-3p overexpression-mediated influences in NSCLC cells. Circ_0006220 could up-regulate GOT2 expression by sponging miR-342-3p. Conclusion: Circ_0006220 promoted the malignant behaviors of NSCLC cells through mediating the miR-342-3p/GOT2 regulation cascade.

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