Downregulation of Inducible Nitric Oxide Synthase Expression in Keloids

Lim, Teik‐Cheng; Moochhala, Shabbir; Tl, Tan; Vj, Chhatwal; Suhumaran, S; Wm, Hon; He, Khoo

doi:10.1097/00006534-199610000-00052

Cited by 5 publications

(1 citation statement)

References 2 publications

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“…16 Furthermore, it has been suggested that keloid formation may be secondary to diminished control of fibroblast collagen production related to the downregulation of iNOS . 17 Our examination of sites of iNOS expression identified macrophages as potential souf ces of NO production. Macrophage iNOS could ,enhance burn wound repair by boosting nonspecific defense mechanisms against pathogens such as bacteria, viruses, and fungi that pose a threat when the integrity of the skin is breached .…”

Section: Discussionmentioning

confidence: 90%

Expression of inducible nitric oxide synthase in human burn wounds

Paulsen

Wurster

Nanney

1998

Wound Repair Regeneration

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Nitric oxide is produced by various cell types and can initiate either beneficial or deleterious effects. Because cultured human keratinocytes express an inducible isoform of nitric oxide synthase, it was postulated that keratinocytes within a burn wound would express increased levels of inducible nitric oxide synthase following the injury. Immunohistochemical staining identified the sites of cellular expression and temporal sequence of inducible nitric oxide synthase protein within partial- and full-thickness burns excised from 29 patients. While migrating keratinocytes at the immediate edge of the wounds showed decreased or undetectable levels of inducible nitric oxide synthase, the immediately adjacent proliferative population and upwardly growing keratinocytes from surviving hair follicles showed increasingly greater cytoplasmic staining for inducible nitric oxide synthase at 4-21 days after injury. Noninjured skin showed minimal inducible nitric oxide synthase staining. Within the wound, detectable inducible nitric oxide synthase protein appeared to decrease as keratinocytes assumed a differentiated phenotype in the outer newly resurfaced epidermis, in inner root sheath layers of hair follicles, or in epithelium of eccrine sweat ducts. Within granulation tissue, immunoreactive inducible nitric oxide synthase was detected in capillary endothelium and in arterial smooth muscle layer. Focal increases in inducible nitric oxide synthase expression were noted in association with inflammatory infiltrates. In conclusion, the cellular and temporal distributions of immunoreactive inducible nitric oxide synthase suggest that nitric oxide may play a role in the regulation of wound repair processes beyond the acute burn injury.

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Section: Discussionmentioning

confidence: 90%