Downregulation of key genes involved in carbon metabolism in <i>Medicago truncatula</i> results in increased lipid accumulation in vegetative tissue

Wijekoon, Champa; Singer, Stacy D.; Weselake, Randall J.; Petrie, James R.; Chen, Guanqun; Singh, Surinder P.; Eastmond, Peter J.; Acharya, S. N.

doi:10.1002/csc2.20124

Cited by 4 publications

(1 citation statement)

References 40 publications

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“…Gene targeted Traits associated with target gene (Wijekoon et al 2020). In soybean, use of virus-or artificial microRNA-mediated gene silencing for GmWPR1, GmExo70J7, GmExo70J8 and GmExo70J9 genes resulted in accelerated leaf senescence and reduced nodule formation (Wang et al 2016b).…”

Section: Cropmentioning

confidence: 99%

Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops

Kumar

Gupta

et al. 2022

Heredity

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In the last decade, advancements in genomics tools and techniques have led to the discovery of many genes. Most of these genes still need to be characterized for their associated function and therefore, such genes remain underutilized for breeding the next generation of improved crop varieties. The recent developments in different reverse genetic approaches have made it possible to identify the function of genes controlling nutritional, biochemical, and metabolic traits imparting drought, heat, cold, salinity tolerance as well as diseases and insect-pests. This article focuses on reviewing the current status and prospects of using reverse genetic approaches to breed nutrient-rich and climate resilient cereal and food legume crops.

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Section: Cropmentioning

confidence: 99%

Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops

Kumar

Gupta

et al. 2022

Heredity

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Transcriptome-guided breeding for Paspalum notatum: producing apomictic hybrids with enhanced omega-3 content

Marino,

Altabe,

Colono

et al. 2024

Theor Appl Genet

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Key message Transcriptomics- and FAME-GC-MS-assisted apomixis breeding generated Paspalum notatum hybrids with clonal reproduction and increased α-linolenic acid content, offering the potential to enhance livestock product's nutritional quality and reduce methane emissions Abstract A low omega-6:omega-3 fatty acid ratio is considered an indicator of the nutritional impact of milk fat on human health. In ruminants, major long-chain fatty acids, such as linoleic acid (18:2, omega-6) and α-linolenic acid (18:3, omega-3), originate from dietary sources and reach the milk via the bloodstream. Since forages are the primary source of long-chain fatty acids for such animals, they are potential targets for improving milk lipid composition. Moreover, a high 18:3 content in their diet is associated with reduced methane emissions during grazing. This work aimed to develop genotypes of the forage grass Paspalum notatum with high leaf 18:3 content and the ability for clonal reproduction via seeds (apomixis). We assembled diploid and polyploid Paspalum notatum leaf transcriptomes and recovered sequences of two metabolism genes associated with the establishment of lipid profiles, namely SUGAR-DEPENDENT 1 ( SDP1 ) and PEROXISOMAL ABC TRANSPORTER 1 ( PXA1 ). Primers were designed to amplify all expressed paralogs in leaves. qPCR was used to analyse SDP1 and PXA1 expression in seven divergent genotypes. Reduced levels of SDP1 and PXA1 were found in the polyploid sexual genotype Q4188. Fatty acid methyl esters/gas chromatography/mass spectrometry (FAME/GC/MS) assays confirmed an increased percentage of 18:3 in this genotype. Crosses between Q4188 and the obligate apomictic pollen donor Q4117 resulted in two apomictic F 1 hybrids (JS9 and JS71) with reduced SDP1 and PXA1 levels, increased 18:3 content, and clonal maternal reproduction. These materials could enhance milk and meat quality while reducing greenhouse gas emissions during grazing. Supplementary Information The online version contains supplementary material available at 10.1007/s00122-024-04788-6.

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Allele mining in diverse accessions of tropical grasses to improve forage quality and reduce environmental impact

et al. 2021

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Background and Aims The C4 Urochloa spp (syn. Brachiaria) and Megathyrsus maximus (syn. Panicum maximum) are used as pasture for cattle across vast areas in tropical agriculture systems in Africa and South America. A key target for variety improvement is forage quality: enhanced digestibility could decrease amount of land required per unit production and enhanced lipid content could decrease methane emissions from cattle. For these traits, loss-of-function (LOF) alleles in known gene targets are predicted to improve them, making a reverse genetics approach of allele mining feasible. We therefore set out to look for such alleles in a diverse Urochloa spp and Megathyrsus maximus accessions from the genebank collection held at CIAT. Methods We studied allelic diversity of 20 target genes (11 for digestibility, 9 for lipid content) in 104 accessions selected to represent genetic diversity and ploidy levels of U. brizantha, U. decumbens, U. humidicola, U. ruziziensis and M. maximum. We used RNAseq and then bait-capture DNA-seq to improve gene models in a U. ruziziensis reference genome to assign polymorphisms with high confidence. Key results We found 953 non-synonymous polymorphisms across all genes and accessions; within these, we identified 7 putative LOF alleles with high confidence, including ones in the non-redundant SDP1 and BAHD01 genes present in diploid and tetraploid accessions. These LOF alleles could respectively confer increased lipid content and digestibility if incorporated into a breeding programme. Conclusions We demonstrated a novel, effective approach to allele discovery in diverse accessions using a draft reference genome from a single species. We used this to find gene variants in a collection of tropical grasses that could help reduce the environmental impact of cattle production.

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Downregulation of key genes involved in carbon metabolism in Medicago truncatula results in increased lipid accumulation in vegetative tissue

Abstract: key genes involved in carbon metabolism in Medicago truncatula results in increased lipid accumulation in vegetative tissue . Crop Science.

Cited by 4 publications

References 40 publications

Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops

Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops

Transcriptome-guided breeding for Paspalum notatum: producing apomictic hybrids with enhanced omega-3 content

Allele mining in diverse accessions of tropical grasses to improve forage quality and reduce environmental impact

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