Downregulation of lncRNA ASMTL-AS1 in Epithelial Ovarian Cancer Correlates with Worse Prognosis and Cancer Progression

Xu, Hui; Tang, Yan; Liu, Lu; Yan, Jie; Qin, Li

doi:10.1055/a-1872-0546

Cited by 3 publications

(2 citation statements)

References 37 publications

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“…The 5-year survival rate of EOC patients is not satisfied. To elucidate the mechanisms underlying EOC metastasis and improve the clinical prognosis, efforts have been made to investigate ncRNA features of EOC [26,27]. However, these ncRNA signatures have not been well implemented clinically.…”

Section: Discussionmentioning

confidence: 99%

Prognostic Value of hsa_circ_0007615 in Epithelial Ovarian Cancer and its Regulatory Effect on Tumor Progression

2023

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This study aimed to interrogate the functional and clinical significance of hsa_circ_0007615 in epithelial ovarian cancer (EOC). GSE192410 was screened for upregulated circRNAs in ovarian cancer. The expression levels of hsa_circ_0007615 were evaluated in a patient cohort comprising 113 EOC tissues and matched normal tissues. Subsequently, the prognostic value was confirmed by the relevance of hsa_circ_0007615 with clinical parameters, Kaplan–Meier analysis and Cox proportional risk model. Cell functional analyses were performed in EOC cell lines using a cell proliferation kit, transwell and cell death kit. Our data revealed that hsa_circ_0007615 was significantly upregulated in EOC tissues and cell lines, compared with normal ones. Multivariate survival analysis revealed that hsa_circ_0007615 emerged as an independent risk factor for overall survival and recurrence of EOC patients. Knockdown of hsa_circ_0007615 in EOC cells led to the blocking of cell proliferation, migration and invasion, but an increase of cell death presenting as ferroptosis. Tumor suppressive effects of hsa_circ_0007615 knockdown can be abolished by miR-874-3p inhibition. TUBB3 was a targeting gene of miR-874-3p. Hsa_circ_0007615 has the functional and clinical significance of EOC. Mechanistically, hsa_circ_0007615 may contribute to EOC by sponging miR-874-3p and moderating TUBB3.

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Section: Discussionmentioning

confidence: 99%

Prognostic Value of hsa_circ_0007615 in Epithelial Ovarian Cancer and its Regulatory Effect on Tumor Progression

2023

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“…[ 7 ] ASMTL‐AS1 strongly decreases cell proliferation, migration, and invasion in epithelial ovarian cancer. [ 8 ] Exosomal ASMTL‐AS1 aggravates the malignancy of residual hepatocellular carcinoma. [ 9 ] However, whether ASMTL‐AS1 affects RCC progression has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

Ursolic acid‐downregulated long noncoding RNA ASMTL‐AS1 inhibits renal cell carcinoma growth via binding to HuR and reducing vascular endothelial growth factor expression

Cai

Zhi

Xie

et al. 2023

J Biochem & Molecular Tox

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It has been reported ursolic acid (UA), one of the naturally abundant pentacyclic triterpenes, possesses a wide range of biological activities including anti‐inflammatory, anti‐atherosclerotic, and anticancer properties. Renal cell carcinoma (RCC) is a severe malignancy due to its asymptomatically spreading ability. Our study aimed to investigate the role and molecular mechanism of UA in RCC. RCC cell proliferation, migration, invasion, and angiogenesis were assessed using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide, Transwell, and tube formation assays. Xenograft tumor models were established to confirm the role of UA and long noncoding RNA ASMTL antisense RNA 1 (ASMTL‐AS1) in vivo. Expression levels of ASMTL‐AS1 and vascular endothelial growth factor (VEGF) were measured using reverse transcriptase quantitative polymerase chain reaction and western blot analysis. The interaction probabilities of ASMTL‐AS1 or VEGF with RNA‐binding protein human antigen R (HuR) were verified by RNA immunoprecipitation experiment. The half‐life period of messenger RNA (mRNA) was determined using actinomycin D. UA inhibited RCC cell growth in vivo and tumorigenesis in vitro. ASMTL‐AS1 was highly expressed in RCC cell lines. Of note, UA downregulated ASMTL‐AS1 expression, and overexpressed ASMTL‐AS1 reversed the UA‐induced suppression on RCC cell migration, invasion, and tube formation. Additionally, ASMTL‐AS1 bound to HuR to maintain the stability of VEGF mRNA. Rescue experiments showed that the suppressed malignancy of RCC cells mediated by ASMTL‐AS1 knockdown was counteracted by overexpression of VEGF. Moreover, silenced ASMTL‐AS1 inhibited RCC tumor growth and metastasis in vivo. The obtained data suggest UA as a promising therapeutic agent to attenuate the development of RCC via regulation of the targeted molecules.

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Long non-coding RNA LINC-PINT as a novel prognostic biomarker in human cancer: a meta-analysis and machine learning

Lin,

Chen,

Zhang

2024

Sci Rep

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Long intergenic non-protein coding RNA, P53 induced transcript (LINC-PINT) exhibits different expression patterns in the majority of tumors, yet its relationship with cancer prognosis remains a subject of debate. This study aims to comprehensively investigate the prognostic significance of LINC-PINT in diverse human cancer. A systematic search was conducted in PubMed, Embase, Cochrane Library, and Web of Science databases to identify pertinent studies exploring the correlation between LINC-PINT expression and cancer patients. Moreover, bioinformatics analysis and in vitro validation were used to validate the results of the meta-analysis and to investigate the potential oncogenic mechanism of LINC-PINT. The meta-analysis encompassed 8 studies, involving 911 patients. The pooled analysis demonstrated a significant association between upregulation of LINC-PINT expression and better survival (P = 0.002) during the cancers. Meanwhile, its downregulation was correlated with advanced tumor staging (P = 0.04) and tumor differentiation (P = 0.03). Additionally, bioinformatics analysis showed that LINC-PINT expression was observed to be linked with Tumor Mutational Burden (TMB) and Microsatellite Instability (MSI) in tumors, the results of bioinformatics were verified by qRT-PCR. And functional enrichment analysis hinted at its involvement in tumorigenesis and tumor progression. Dysregulated LICN-PINT expression is associated with the clinical prognostic and pathological features of various cancers, exhibiting substantial potential as a novel prognostic biomarker.

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Downregulation of lncRNA ASMTL-AS1 in Epithelial Ovarian Cancer Correlates with Worse Prognosis and Cancer Progression

Cited by 3 publications

References 37 publications

Prognostic Value of hsa_circ_0007615 in Epithelial Ovarian Cancer and its Regulatory Effect on Tumor Progression

Prognostic Value of hsa_circ_0007615 in Epithelial Ovarian Cancer and its Regulatory Effect on Tumor Progression

Ursolic acid‐downregulated long noncoding RNA ASMTL‐AS1 inhibits renal cell carcinoma growth via binding to HuR and reducing vascular endothelial growth factor expression

Long non-coding RNA LINC-PINT as a novel prognostic biomarker in human cancer: a meta-analysis and machine learning

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