Downregulation of miR-101 contributes to epithelial-mesenchymal transition in cisplatin resistance of NSCLC cells by targeting ROCK2

Ye, Zhiqiang; Yin, Shuo; Su, Zhongzhen; Bai, Man; Zhang, Haibo; Hei, Ziqing; Cai, Sanjun

doi:10.18632/oncotarget.6852

Cited by 51 publications

(38 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Accumulating researches had reported that the abnormal expression of miR-101 was involved in cell proliferation, colony formation, migration and apoptosis through targeting some genes [21,22]. Given its signi cant functions in controlling the biological behaviors of tumor cells, miR-101 was considered as a promising candidate bio-marker for therapy and prognosis of some cancers [23,24]. For examples, the study of Li et al reported that the expression of miR-101 was decreased in laryngeal squamous cell carcinoma tissues, and showed negative association with high tumor grade, lymph node metastasis as well as advanced clinical stage [25].…”

Section: Discussionmentioning

confidence: 99%

microRNA-101 Represents an Innovative Bio-Marker in Oral Cancer Detection

Wang

Song

Yang

2020

Preprint

View full text Add to dashboard Cite

Background: It had been suggested that microRNA-101 (miR-101) was involved in carcinogenesis and progression of various human tumors. The purpose of this study was to investigate the diagnostic value of miR-101 in oral cancer.Methods: Quantitative real-time polymerase chain reaction (QRT-PCR) was used to detect the serum mRNA level of miR-101 in 130 oral cancer cases and 82 healthy individuals. The association of miR-101 with clinical characteristics of oral cancer patients was estimated using chi-square test. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic significance of miR-101 in oral cancer.Results: Compared with healthy controls, serum miR-101 level was significantly down-regulated in oral cancer patients (P<0.001). Furthermore, low expression of miR-101 was closely associated with histological grade (P=0.037), TNM stage (P=0.018) and lymph node metastasis (P=0.023). ROC curve analysis indicated that serum miR-101 could effectively distinguish oral cancer patients from healthy controls with an area under the curve (AUC) of 0.878 (95%CI=0.834-0.922, P<0.001). The cutoff level of miR-101 expression for oral cancer diagnosis was 1.46, with the sensitivity of 82.9% and the specificity of 74.6%.Conclusions: Decreased expression of miR-101 is correlated with the aggressive progression of oral cancer. Serum miR-101 may be a promising bio-marker for early diagnosis of oral cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

microRNA-101 Represents an Innovative Bio-Marker in Oral Cancer Detection

Wang

Song

Yang

2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Cancer cells can hijack signaling by over-expressing or mutating growth factor receptors to increase proliferative signals and miRNAs target certain receptor to modulate signaling. [187][188][189][190][191][192] let-7c-5p Decreased N/K-RAS, ABCC2, Bcl-XL, ITGB3, MAP4K3, HOXA1 [122,[193][194][195][196] miR-101-3p Decreased ZEB1, ROCK2, MALAT-1, EZH2 [48,180,[197][198][199] target epidermal growth factor (EGFR) (Figure 3), which is commonly overexpressed in NSCLC (Table 1), to alter downstream signaling molecules such as AKT and ERK1/2 and decrease the growth phenotype. In addition, miR-139-5p, 58 miR-30a-5p, 59 miR-140-3p, 60 miR-320a-3p 61 and miR-195-5p 62 all target insulin-like growth factor 1 receptor (IGF1R), while miR-486-5p directly targets both IGF1R and its ligand insulin-like growth factor 1 (IGF1) 63 and miR-135a-5p targets only IGF1.…”

Section: Signal Transduction In Lung Cancer Survival and Proliferationmentioning

confidence: 99%

MicroRNAs in non‐small cell lung cancer: Gene regulation, impact on cancer cellular processes, and therapeutic potential

Petrek

2019

Pharmacology Res & Perspec

View full text Add to dashboard Cite

Lung cancer remains the most lethal cancer among men and women in the United States and worldwide. The majority of lung cancer cases are classified as non‐small cell lung cancer (NSCLC). Developing new therapeutics on the basis of better understanding of NSCLC biology is critical to improve the treatment of NSCLC. MicroRNAs (miRNAs or miRs) are a superfamily of genome‐derived, small noncoding RNAs that govern posttranscriptional gene expression in cells. Functional miRNAs are commonly dysregulated in NSCLC, caused by genomic deletion, methylation, or altered processing, which may lead to the changes of many cancer‐related pathways and processes, such as growth and death signaling, metabolism, angiogenesis, cell cycle, and epithelial to mesenchymal transition, as well as sensitivity to current therapies. With the understanding of miRNA biology in NSCLC, there are growing interests in developing new therapeutic strategies, namely restoration of tumor suppressive miRNAs and inhibition of tumor promotive miRNAs, to combat against NSCLC. In this article, we provide an overview on the molecular features of NSCLC and current treatment options with a focus on pharmacotherapy and personalized medicine. By illustrating the roles of miRNAs in the control of NSCLC tumorigenesis and progression, we highlight the latest efforts in assessing miRNA‐based therapies in animal models and discuss some critical challenges in developing RNA therapeutics.

show abstract

“…9 Furthermore, miRNAs play a critical role in various cancer cellular processes, such as development, proliferation, apoptosis, migration, invasion, and drug resistance. 10 To identify the biological role of miRNAs in gefitinibresistant cells, we examined the genome-wide miRNA expression in the parental HCC827 cells and gefitinibresistant HCC827/GR-8-1 cells. Previous studies showed that miRNAs regulate the EGFR gene pathway and are a predictor of response to EGFR-tyrosine kinase inhibitor (TKI) therapy in cancer.…”

Section: Micro-rna and Lung Cancermentioning

confidence: 99%

Genome-wide profiling of micro-RNA expression in gefitinib-resistant human lung adenocarcinoma using microarray for the identification of miR-149-5p modulation

Qin

Yan

et al. 2017

Tumour Biol.

View full text Add to dashboard Cite

To understand the mechanism involved in gefitinib resistance, we established gefitinib-resistant human HCC827/GR-8-1 cell line from the parental HCC827 cell line. We compared the micro-RNA expression profiles of the HCC827 cells HCC827/GR-8-1 using Agilent micro-RNA microarrays. The micro-RNAs, such as the miR-149-5p, were up-or downregulated and associated with acquired gefitinib resistance. Quantitative real-time polymerase chain reaction was then performed to verify the expression patterns of different micro-RNAs. The result showed that miR-149-5p was upregulated in the HCC827/GR-8-1 cell line. To investigate the biological function of miR-149-5p in non-small cell lung cancer cells acquired gefitinib resistance, we examined cell proliferation using a cell counting kit-8 assay. Cell viability was evaluated after the miR-149-5p mimics, inhibitors, and negative control were separately transfected into the non-small cell lung cancer cells. The results showed that the non-small cell lung cancer cells transfected with miR-149-5p mimics exhibited reduced cell motility. The drug-sensitivity assay results revealed that the overexpression of miR-149-5p effectively evaluates the half maximal inhibitory concentration values of the cell in response to gefitinib, and the downregulation of miR-149-5p can attenuate the half maximal inhibitory concentration values of the cell lines in response to gefitinib. Furthermore, the levels of miR-149-5p in the HCC827 and HCC827/GR-8-1 cells were inversely correlated with caspase-3 expression. In conclusion, this study revealed that miR-149-5p is upregulated in the HCC827/ GR-8-1 cells and involved in the acquired gefitinib resistance.

show abstract

Downregulation of miR-101 contributes to epithelial-mesenchymal transition in cisplatin resistance of NSCLC cells by targeting ROCK2

Cited by 51 publications

References 30 publications

microRNA-101 Represents an Innovative Bio-Marker in Oral Cancer Detection

microRNA-101 Represents an Innovative Bio-Marker in Oral Cancer Detection

MicroRNAs in non‐small cell lung cancer: Gene regulation, impact on cancer cellular processes, and therapeutic potential

Genome-wide profiling of micro-RNA expression in gefitinib-resistant human lung adenocarcinoma using microarray for the identification of miR-149-5p modulation

Contact Info

Product

Resources

About