Downregulation of miR‑10b‑5p facilitates the proliferation of uterine leiomyosarcoma cells: A microRNA sequencing‑based approach

Abstract

Uterine leiomyosarcoma (ULMS) is one of the most aggressive gynecological malignancies. In addition, the molecular background of ULMS has not been fully elucidated due to its low incidence. Therefore, no effective treatment strategies have been established based on its molecular background. The present study aimed to investigate the roles of microRNAs (miRNAs/miRs) in the development of ULMS. Comprehensive miRNA sequencing was performed using six ULMS and three myoma samples, and revealed 53 and 11 significantly upregulated and downregulated miRNAs, respectively. One of the most abundant miRNAs in myoma samples was miR-10b-5p. The mean normalized read count of miR-10b-5p was 93,650 reads in myoma, but only 27,903 reads in ULMS. Subsequently, to investigate the roles of miR-10b-5p, gain-of-function analysis was performed using SK-UT-1 and SK-LMS-1 cell lines. The overexpression of miR-10b-5p suppressed cell proliferation and reduced the number of colonies. Moreover, miR-10b-5p increased the number of cells in the G 1 phase. In conclusion, tumor-suppressive miR-10b-5p was significantly downregulated in ULMS compared with in myoma; thus, miR-10b-5p may serve a specific role in sarcoma progression.

Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progression

“…According to previous papers (49–50), comprehensive miRNA sequencing was performed. Sample EVs were isolated from MCF-7 cells, MCF-7 PSAT1 OE cells, BM02 cells and BM02 PSAT1 KD cells.…”

Comprehensive Review of Uterine Leiomyosarcoma: Pathogenesis, Diagnosis, Prognosis, and Targeted Therapy

“…Among them, the function of several miRNAs in uLMS has been characterized. miR-10b-5p significantly inhibited cell proliferation, reduced the number of colonies, and increased the number of uLMS cells in the G1 phase [145]. miR-130b promoted aggressiveness in LMS cells by accelerating tumor growth and enhancing metastatic potential [150].…”

“…Among several types of non-coding RNAs, intercellular miRNAs have been extensively investigated and demonstrated their important roles in cell signaling, proliferation, migration, and angiogenesis in LMS development [144] (Table 4). A few comparison studies between myometrium and LMS at tissue and cell levels identified many differentially expressed miRNAs [145][146][147][148][149]. Among them, the function of several miRNAs in uLMS has been characterized.…”