Downregulation of miR-136-5p in hepatocellular carcinoma and its clinicopathological significance

Abstract

The clinical significance of microRNA (miR)-136-5p in hepatocellular carcinoma (HCC) has not been verified. Therefore, in the current study, the authors aimed to explore miR-136-5p expression and its clinical significance in HCC, as well as to investigate its potential target genes function. The authors detected the levels of miR-136-5p in 101 pairs of HCC and para-cancer tissues via reverse transcription-quantitative polymerase chain reaction. Gene Expression Omnibus database and the Cancer Genome Atlas (TCGA) database were used to further verify the clinical significance of miR-136-5p expression in HCC. The target genes prediction analysis of miR-136-5p, natural language processing (NLP) analysis of HCC in PubMed and gene functional enrichment analysis were conducted. The miR-136-5p level was markedly downregulated in HCC tissue, compared to para-non-tumor tissue. MiR-136-5p expression decreased in HCC patients with metastasis (P=0.004), advance TNM stage (P<0.001), portal vein tumor embolus (P=0.007) and vaso-invasion (P=0.003), compared with those HCC patients with non-metastasis, early TNM stage, non-portal vein tumor embolus and non-vaso-invasion, respectively. In the TCGA database, downregulated miR-136-5p was also observed in HCC tissue compared to normal liver tissue (P<0.001). There were 178 genes obtained from the overlap between predicted targets and NLP analysis. GO and KEGG pathway analyses revealed some significant pathways related to cancers. Downregulation of miR-136-5p may be responsible for the carcinogenesis and aggressiveness of HCC. miR-136-5p may act as an anti-carcinoma miRNA, which is essential for HCC progression through the regulation of various signaling pathways. Thus, miR-136-5p interaction may provide a novel strategy for HCC treatment.

Silencing of long noncoding RNA LEF1‐AS1 prevents the progression of hepatocellular carcinoma via the crosstalk with microRNA‐136‐5p/WNK1

“…Additionally, in the context of colorectal cancer, it was discovered that the elevated lncRNA LEF1-AS1 expression has correlated to shorter periods of overall survival and recurrence-free survival of patients (Chen, Yu, Xu, & Shen, 2017). As a predicted downstream miR according to bioinformatics analysis, miR-136-5p shows therapeutic significance in HCC by functioning as a tumor suppressor through a variety of pathways, yet the detailed regulatory mechanism still awaits further investigation (Ding et al, 2017). Besides, miR-136-5p interacts with lncRNA CRNDE to potentiate the glioma malignancy via B-cell lymphoma-2 and Wnt2 (Li et al, 2017).…”

Exosomes derived from human umbilical cord MSCs rejuvenate aged MSCs and enhance their functions for myocardial repair

“…MiR-136 was initially identified by cloning studies in mouse, and later verified in human embryonic stem cells [38]. Researches have shown that miR-136 acts as an anti-carcinoma miRNA in lung [39] and hepatocellular carcinoma [40], and involves the metastasis of colon cancer by suppressing epithelial-to-mesenchymal transition [41]. Here, we showed that both the cellular and exosomal level of miR-136 were higher in UMSCs than in OMSCs.…”

Exosomes derived from human umbilical cord MSCs rejuvenate aged MSCs and enhance their functions for myocardial repair

“…MiR-136 was initially identified by cloning studies in mouse, and later verified in human embryonic stem cells [34]. Researches shown that miR136 acts as an anti-carcinoma miRNA in lung [35] and hepatocellular carcinoma [36], and involves the metastasis of colon cancer by suppressing epithelialto-mesenchymal transition [37]. Here, we showed that both cellular and exosomal level of miR-136 were higher in UMSCs than in OMSCs.…”