Downregulation of miR‐24‐3p promotes osteogenic differentiation of human periodontal ligament stem cells by targeting SMAD family member 5

Li, Zhaobao; Sun, Yangying; Cao, Sumin; Zhang, Jing; Wei, Jianming

doi:10.1002/jcp.27499

Cited by 19 publications

(11 citation statements)

References 31 publications

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“…MiR-24 also participates in erythropoiesis [29]. miR-24-3p controls osteogenic differentiation via regulating SMAD family member 5 (Smad5) in human periodontal ligament stem cells (hPDLSCs) [30]. Previous studies have demonstrated that miR-24-3p can promote the proliferation of several types of cancer cells [25,31,32,33,34,35], which contradicts our results.…”

Section: Discussioncontrasting

confidence: 79%

Bta-miR-24-3p Controls the Myogenic Differentiation and Proliferation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting ACVR1B

Xing

Ren

et al. 2019

Animals

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Simple SummaryMicroRNAs play pivotal roles in skeletal muscle development, but the molecular basis of their functions in fetal bovine skeletal muscle development is largely unknown. Here, we report a mechanistic study of bta-miR-24-3p, a key miRNA regulator of the myogenic differentiation of fetal bovine platelet-derived growth factor receptor alpha negative (PDGFRα-) progenitor cells. We isolated progenitor cells from the bovine fetal longissimus dorsi muscle and purified them with PDGFRα antibodies to remove fibro-adipogenic progenitors. We observed elevated bta-miR-24-3p expression during differentiation, and bta-miR-24-3p overexpression led to promoted myogenic differentiation but suppressed proliferation. Moreover, activin receptor type 1B (ACVR1B) was identified as a direct target of bta-miR-24-3p, and ACVR1B-silencing cells exhibited similar phenotypes to bta-miR-24-3p-overexpressing bovine PDGFRα- progenitor cells. These results extended our understanding on the roles of miRNA in fetal muscle development. The method of removing fibro-adipogenic progenitors in our study will also provide useful information for other investigators.AbstractMicroRNAs modulate a variety of cellular events, including skeletal muscle development, but the molecular basis of their functions in fetal bovine skeletal muscle development is poorly understood. In this study, we report that bta-miR-24-3p promotes the myogenic differentiation of fetal bovine PDGFRα- progenitor cells. The expression of bta-miR-24-3p increased during myogenic differentiation. Overexpression of bta-miR-24-3p significantly promoted myogenic differentiation, but inhibited proliferation. A dual-luciferase assay identified ACVR1B as a direct target of bta-miR-24-3p. Similarly, knocking down ACVR1B by RNA interference also significantly inhibited proliferation and promoted the differentiation of bovine PDGFRα- progenitor cells. Thus, our study provides a mechanism in which bta-miR-24-3p regulates myogenesis by inhibiting ACVR1B expression.

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Section: Discussioncontrasting

confidence: 79%

Bta-miR-24-3p Controls the Myogenic Differentiation and Proliferation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting ACVR1B

Xing

Ren

et al. 2019

Animals

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“…miR - 24 - 3p , which belongs to the miR - 24 family, is a well-known cancer-associated miRNA that is differentially expressed in the initiation and progression in multiple kinds of tumors. Inhibition of miR - 24 - 3p promotes osteogenic differentiation by targeting Smad5 [17]. miR - 24 - 3p also facilitates cell proliferation and inhibits cell apoptosis in gastric cancer by targeting BCL2L11 [18].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-24-3p regulates neuronal differentiation by controlling hippocalcin expression

Kang

Park

Han

2019

Cell. Mol. Life Sci.

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Hippocalcin (HPCA) is a neuron-specific calcium-binding protein predominantly expressed in the nervous system. In the present study, we demonstrate that HPCA regulates neuronal differentiation in SH-SY5Y cells. We observed that the expression level of HPCA was increased during neuronal differentiation. Depletion of HPCA inhibited both neurite outgrowth and synaptophysin (SYP) expression, whereas overexpression of HPCA enhanced neuronal differentiation. Interestingly, we also found that the expression of HPCA mRNA was modulated by miR-24-3p. Using a dual-luciferase assay, we showed that co-transfection of a plasmid containing the miR-24-3p binding site from the 3′-untranslated region (3′UTR) of the HPCA gene and an miR-24-3p mimic effectively reduced luminescence activity. This effect was abolished when miR-24-3p seed sequences in the 3′UTR of the HPCA gene were mutated. miR-24-3p expression was decreased during differentiation, suggesting that the decreased expression level of miR-24-3p might have upregulated mRNA expression of HPCA. As expected, upregulation of miR-24-3p by an miRNA mimic led to reduced HPCA expression, accompanied by diminished neuronal differentiation. In contrast, downregulation of miR-24-3p by an antisense inhibitor promoted neurite outgrowth as well as levels of SYP expression. Taken together, these results suggest that miR-24-3p is an important miRNA that regulates neuronal differentiation by controlling HPCA expression.Electronic supplementary materialThe online version of this article (10.1007/s00018-019-03290-3) contains supplementary material, which is available to authorized users.

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“…During osteogenic differentiation of PDLSCs, the expression of miR-21 and miR-24-3p decrease, and their downregulation markedly inhibits osteogenesis of hPDLSCs by targeting SMAD family member 5 (Smad5)[ 125 , 126 ]. miR-21 also performs a positive function in mediating the stretch-induced osteogenic differentiation of hPDLSCs by regulating the expression of activin receptor type IIB[ 127 ].…”

Section: Multiple Factors Influencing the Odontogenic/osteogenic Differentiation Of Dmscsmentioning

confidence: 99%

Multidifferentiation potential of dental-derived stem cells

Yin¹,

Luo²,

Feng³

et al. 2021

WJSC

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Tooth-related diseases and tooth loss are widespread and are a major public health issue. The loss of teeth can affect chewing, speech, appearance and even psychology. Therefore, the science of tooth regeneration has emerged, and attention has focused on tooth regeneration based on the principles of tooth development and stem cells combined with tissue engineering technology. As undifferentiated stem cells in normal tooth tissues, dental mesenchymal stem cells (DMSCs), which are a desirable source of autologous stem cells, play a significant role in tooth regeneration. Researchers hope to reconstruct the complete tooth tissues with normal functions and vascularization by utilizing the odontogenic differentiation potential of DMSCs. Moreover, DMSCs also have the ability to differentiate towards cells of other tissue types due to their multipotency. This review focuses on the multipotential capacity of DMSCs to differentiate into various tissues, such as bone, cartilage, tendon, vessels, neural tissues, muscle-like tissues, hepatic-like tissues, eye tissues and glands and the influence of various regulatory factors, such as non-coding RNAs, signaling pathways, inflammation, aging and exosomes, on the odontogenic/osteogenic differentiation of DMSCs in tooth regeneration. The application of DMSCs in regenerative medicine and tissue engineering will be improved if the differentiation characteristics of DMSCs can be fully utilized, and the factors that regulate their differentiation can be well controlled.

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Downregulation of miR‐24‐3p promotes osteogenic differentiation of human periodontal ligament stem cells by targeting SMAD family member 5

Cited by 19 publications

References 31 publications

Bta-miR-24-3p Controls the Myogenic Differentiation and Proliferation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting ACVR1B

Bta-miR-24-3p Controls the Myogenic Differentiation and Proliferation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting ACVR1B

MicroRNA-24-3p regulates neuronal differentiation by controlling hippocalcin expression

Multidifferentiation potential of dental-derived stem cells

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