Downregulation of miR-29c-3p is associated with a poor prognosis in patients with laryngeal squamous cell carcinoma

Abstract

BackgroundLaryngeal squamous cell carcinoma (LSCC) is considered to be a common malignancy of the head and neck with poor prognosis for its late diagnosis, metastasis and recurrence. Growing evidence demonstrates that the dysregulation of miR-29c-3p (microRNA-29c-3p) plays an important role in various tumor processes. Our study investigates the expression of miR-29c-3p in LSCC and analyzes the correlation of its dysregulation with clinicopathologic parameters and prognosis.MethodsThe expression of hsa-miR-29c-3p in LSCC tissues and the adjacent normal laryngeal tissues was detected in 96 LSCC formalin-fixed paraffin-embedded tissues by quantitative real-time PCR (qRT-PCR). The SPSS statistical software package (17.0) was used to analyze the associations between miR-29c-3p expressions and various clinicopathological characteristics. The overall survival (OS) was analyzed by the Kaplan-Meier method and log-rank test, and we analyzed the independent factor of prognosis by Cox proportional hazard analysis.ResultsA downregulation of miR-29c-3p expression in LSCC was significantly correlated with smoking index, tumor size, tumor site, differentiation, T classification, TNM stage, and lymph node metastasis (P < 0.05), but there was no correlation with age and alcohol consumption (P > 0.05). In the multivariate survival analysis, low miR-29c-3p expression was associated with shorter overall survival (P < 0.05). Furthermore, miR-29c expression was an independent prognostic factor for laryngeal cancer patients.ConclusionsMiR-29c-3p has different expression levels at different stages of tumor progression, suggesting that miR-29c-3p may be a promising biomarker for evaluating the progression of LSCC and the prognosis of patients with LSCC. MiR-29c-3p can also be a novel molecular target for anti-laryngeal cancer therapy.

The Construction and Analysis of Tumor-Infiltrating Immune Cells and ceRNA Networks in Bladder Cancer

“…Therefore, we inferred that hsa-miR-29c-3p, ELN, DSC2, neutrophils, and Tfh might play crucial roles in the progression of BLCA. miR-29c-3p, which was reported as a tumor suppressor in the miRNAs family (Schmitt et al, 2013), plays a protective role in various malignancies, such as head and neck cancers (Liu et al, 2013;Hudcova et al, 2016;Fang et al, 2019), gastrointestinal cancers (Ding et al, 2011;Matsuo et al, 2013;Chen et al, 2017), hepatobiliary cancers (Wang et al, 2015;Shu et al, 2017), breast cancer (Bhardwaj et al, 2017), and BLCA (Inamoto et al, 2018). Numerous studies have shown that the low expression level of miR-29c was associated with worse tumor differentiation, advanced disease stage, and poor prognosis.…”

MicroRNA-1298 is downregulated in non-small cell lung cancer and suppresses tumor progression in tumor cells

“…*P < 0.05, **P < 0.01 ***P < 0.001 4709 expression in colon adenocarcinoma predicts a poor prognosis in cancer patients [26]. Downregulation of miR-29c-3p is associated with the poor overall survival in patients with laryngeal squamous cell carcinoma [27]. Overexpression of miR-193b in glioma patients serves as a candidate diagnostic and prognostic biomarker [28].…”

Long Non-Coding RNA MEG3 Modifies Cell-Cycle, Migration, Invasion, and Proliferation Through AKAP12 by Sponging miR-29c in Meningioma Cells

“…In addition, miR-29c acted as cancer-associated miRNA and could modulate the tumorigenesis of multiple human carcinomas. For instance, miR-29c deletion was tightly implicated in poor prognosis in laryngeal squamous cell carcinoma ( 31 ). Also, another report presented that miR-29c retarded cell migration and invasion by inactivating cyclin-dependent kinase 6 (CDK6) in gastric cancer ( 32 ).…”