Downregulation of miR-320 Alleviates Endoplasmic Reticulum Stress and Inflammatory Response in 3T3-L1 Adipocytes

Abstract

Objective MicroRNAs serve important roles in the regulation of endoplasmic reticulum stress (ERs). This study aimed to investigate the role of microRNA-320 (miR-320) in the development of ERs and the inflammatory response in 3T3-L1 adipocytes. Materials and Methods The adipose tissue expression levels of miR-320 and ERs markers (GRP78, GRP94, Derlin-1 and CHOP) and the serum concentration of inflammatory cytokines (TNF-α, NF-κB and IL-6) in obese patients were evaluated using quantitative real-time RT-PCR. The correlation of miR-320 with genes involved in ERs and inflammation was analyzed. The effects of miR-320 on ERs and inflammation were explored using mature 3T3-L1 adipocytes, which were pretreated with palmitic acid (PA). Results ERs markers and inflammatory cytokines were all upregulated in obese patients. Adipose tissue miR-320 expression was also increased in obese patients, and had positive correlations with the levels of ERs markers and inflammatory cytokines. After PA treatment, the levels of ERs markers and inflammatory cytokines were elevated significantly in 3T3-L1 adipocytes. Moreover, miR-320 expression was increased in the cells under ERs status. The upregulation of miR-320 could enhance the expression of ERs markers and inflammatory cytokines, but the downregulation of miR-320 resulted in the opposite results. Conclusion The data of this study indicate that miR-320 expression is upregulated in ERs status, and the downregulation of miR-320 ameliorates ERs and the inflammatory response in 3T3-L1 adipocytes. We consider that the approaches to decrease miR-320 expression may be novel therapeutic strategies for the treatment of obesity and obesity-related diseases.

Detection of SARS-CoV-2 Derived Small RNAs and Changes in Circulating Small RNAs Associated with COVID-19

“…The diagrams in Figure 3 describe the detailed read abundance for the top six differentially abundant circ-miRNAs: has-miRNA320a-3hashsa-miRNA629-5p, hsa-miRNA29a-3p, has-miRNA-324-3p, has-miRNA-185-5hasnd hsa-miRNA4516, whereby the first five of the six clearly distinguish mild from severe cases in our cohort. hsa-miRNA320 ( Figure 3 ) was reported to be unregulated, in association with heart failure in patients with diabetes mellitus [ 35 , 36 , 37 ]. The entire subset of miRNA320 (b,c,d) was found to be up-regulated in severe cases here.…”

The MiR-320 Family Is Strongly Downregulated in Patients with COVID-19 Induced Severe Respiratory Failure

“…Interestingly, overexpression of miR-320-5p resulted in a marked reduction in TNF-α, IL-1β, IL-6 and IL-8 levels by targeting tumor necrosis factor receptor-associated factor 3 (TRAF). The role of miR320 in the regulation of IL-6 and TNF-α has also been described in the inflammatory response in 3T3-L1 adipocytes and in smoke inhalation injury rats [ 31 , 32 ]. In addition, miR-320 expression is significantly decreased in the inflamed mucosa of patients with ulcerative colitis and Crohn’s disease [ 33 ].…”

Epigenetic signatures underlying inflammation: an interplay of nutrition, physical activity, metabolic diseases, and environmental factors for personalized nutrition

“…Meanwhile, miR-130b contributed to obesity-associated adipose tissue inflammation and insulin resistance in diabetic rodents [98]. Endoplasmic reticulum stress and inflammatory markers were up-regulated in obese patients, showing positive correlations with miR-320 expression in adipose tissue [99]. miR-221 triggered white adipose tissue inflammation and insulin resistance in obesity partially through suppressing SIRT1 [100].…”