Downregulation of miR-451a in plasma may promote prothrombin expression and contribute to high blood coagulation state in gestational diabetes mellitus

Abstract

Purpose: To investigate F2 and miR-451a levels in gestational diabetes mellitus (GDM) patients, evaluate the potential role of miR-451a in GDM, and to explore its possible downstream targets.
 Methods: Thromboelastography (TEG) analysis was conducted to analyze coagulation differences between the GDM and control groups. Quantitative polymerase chain reaction (qPCR) and immunoblot were performed to analyze miR-451a and F2 levels after exposure to high glucose and miR-451a mimics or an miR-451a inhibitor. Luciferase assay was used to explore the potential regulatory mechanism.
 Results: Enhancement of the patients' blood coagulation ability and down-regulated miR-451a expression levels occurred in the plasma of GDM patients. The data further show that hepatocytes exposed to high glucose reduced miR-451a levels and enhanced F2 expression. The overexpression of miR-451a inhibited the expression of F2.
 Conclusion: MiR-451a may serve as a promising marker for hypercoagulable state GDM patients
 Keywords: Gestational diabetes mellitus (GDM), MiR-451a, F2, Hypercoagulable state, Prothrombin, Thromboelastography (TEG)

Dexmedetomidine alleviates microglial activation of neuropathic pain by modulating miR-23a/PDE10A axis in streptozotocin-induced diabetic mice

“…As a class of noncoding RNAs (18)(19)(20)(21)(22) nucleotides in length) [2], miRNAs act as gene suppressors, inhibiting expression of target mRNAs by targeting the 3'-untranslated regions (UTRs) [3]. Results of microarray analyses have revealed the alterations in the miRNAs expressions in streptozotocin (STZ)-induced DM mice, indicating that the abnormal miRNA expression profiling in DM progression [4].…”