Downregulation of miR-4755-5p promotes fluoride-induced osteoblast activation via tageting Cyclin D1

Gao, Jiayu; Yu, Qiang; Luo, Keke; Wang, Xilan; Yu, Chun; Zhang, Aihua; Pan, Xueli

doi:10.1016/j.jtemb.2020.126626

Cited by 6 publications

(11 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There were no significant differences in gender, smoking, or drinking among the three subgroups. Detailed demographic characteristics and values have been reported in our previous study 25 . It was also confirmed in our previous study that the markers of osteogenic activation BGP and ALP elevated with the increase in UF concentration in fluoride‐exposed population.…”

Section: Resultssupporting

confidence: 85%

“…Bioinformatics analyses showed that CyclinD1 and TGF‐β1 were targets of miR‐486‐3p. Our previous study examined the expression of CyclinD1 among the fluoride‐exposed population and showed that CyclinD1 gene mRNA and protein levels increased as the concentration of UF increased 25 . In this study, with increased UF levels, the mRNA and protein levels of TGF‐β1 also increased (Figure 1(B),(C)).…”

Section: Resultssupporting

confidence: 63%

“…Sodium fluoride (NaF) (0, 600, or 1200 μmol/L; Sigma) was chosen for subsequent experiments according to the previous finding that treatment with 1200 μmol/L NaF for 72 h led to more viability of osteoblasts 25 ,35 In overexpression experiments, osteoblasts were transiently transfected prior to treatment with NaF at 0 or 1200 μmol/L.…”

Section: Methodsmentioning

confidence: 99%

“…The total protein from human osteoblasts was extracted in the RIPA buffer, and the proteins in the nucleus and the cytoplasm were obtained by the Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime, China). Western blotting was performed as described previously 25 . In brief, SDS‐PAGE was conducted for separating proteins and then transferred onto PVDF membranes.…”

Section: Methodsmentioning

confidence: 99%

“…We adopted bioinformatics tools (miRWalk 3.0 database and InteractiVenn virtual tool) and found three of the 83 downregulated miRNAs including miR‐486‐3p, miR‐4755‐5p, and let‐7c‐5p, with CyclinD1 as their target. These three miRNAs were all investigated and the results of miR‐4755‐5p as well as let‐7c‐5p has been published 25,26 . In this paper, we only reported miR‐486‐3p research results.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

miR‐486‐3p regulates CyclinD1 and promotes fluoride‐induced osteoblast proliferation and activation

Ouyang

Yu²,

Luo

et al. 2021

Environmental Toxicology

Self Cite

View full text Add to dashboard Cite

Fluoride is a persistent environmental pollutant, and its excessive intake contributes to skeletal and dental fluorosis. The mechanisms underlying fluoride‐induced abnormal osteoblast proliferation and activation, which are related to skeletal fluorosis, have not yet been fully clarified. As important epigenetic regulators, microRNAs (miRNAs) participate in bone metabolism. On the basis of our previous miRNA‐seq results and bioinformatics analysis, this study investigated the role and specific molecular mechanism of miR‐486‐3p in fluoride‐induced osteoblast proliferation and activation via CyclinD1. Herein, in the fluoride‐challenged population, we observed that miR‐486‐3p expression decreased while CyclinD1 and transforming growth factor (TGF)‐β1 increased, and miR‐486‐3p level correlated negatively with the expression of CyclinD1 and TGF‐β1 genes. Further, we verified that sodium fluoride (NaF) decreases miR‐486‐3p expression in human osteoblasts and overexpression of miR‐486‐3p reduces fluoride‐induced osteoblast proliferation and activation. Meanwhile, we demonstrated that miR‐486‐3p regulates NaF‐induced upregulation of CyclinD1 by directly targeting its 3′‐untranslated region (3′‐UTR). In addition, we observed that NaF activates the TGF‐β1/Smad2/3/CyclinD1 axis and miR‐486‐3p mediates transcriptional regulation of CyclinD1 by TGF‐β1/Smad2/3 signaling pathway via targeting TGF‐β1 3′‐UTR in vitro. This study, thus, contributes significantly in revealing the mechanism of miR‐486‐3p‐mediated CyclinD1 upregulation in skeletal fluorosis and sheds new light on endemic fluorosis treatment.

show abstract

Section: Resultssupporting

confidence: 85%

Section: Resultssupporting

confidence: 63%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations