Downregulation of MMP1 in MDS-Derived Mesenchymal Stromal Cells Reduces the Capacity to Restrict MDS Cell Proliferation

Zhao, Shuyun; Zhao, Yan; Guo, Jiang; Fei, C.; Zheng, Qiusheng; Li, X.; Chang, Chia-Chuan

doi:10.1016/s0145-2126(17)30305-3

Cited by 2 publications

(2 citation statements)

References 23 publications

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“…The results of this study indicate that MSC-WJ therapy has not been effective in reducing MMP-1 levels within 3 weeks after injection. Possibly in this period, MSC-WJ was still synthesising and releasing MMP-1 which is needed for the apoptosis of chondrocyte and synovial cells [ 20 ], the process of MSC migration and proliferation [ 16 ] and differentiation. Ho et al , (2009) showed that MMP-1 plays an important role in the MSC migration function, which operates through MMP1-PAR1 axis signalling [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Mesenchymal Stem Cell Wharton's Jelly on Matrix Metalloproteinase-1 and Interleukin-4 Levels in Osteoarthritis Rat Model

Endrinaldi¹,

Darwin²,

Zubir³

et al. 2019

Open Access Maced J Med Sci

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BACKGROUND: Osteoarthritis (OA) is generally considered a degenerative joint disease caused by biomechanical changes and the ageing process. In OA pathogenesis, the development of OA is thought to be regulated largely by excess matrix metalloproteinase (MMP), which contributes to the degradation of extracellular matrices such as MMP-1 and Interleukin-4. AIM: This study aims to prove the influence of Mesenchymal Stem Cell Wharton Jelly on decreasing MMP-1 levels and increasing IL-4 which is a specific target as a target component in cases of osteoarthritis in vivo. MATERIAL AND METHODS: This research is an experimental study with the design of Post-Test-Only Control Group Design. The sample consisted of 16 OA rats as a control group and 16 OA rats treated with MSC-WJ as a treatment group. OA induction is done by injection of monosodium iodoacetate (MIA) into the intra-articular right knee. Giving MSC-WJ is done in the third week after MIA induction. The serum MMP-1 and IL-4 levels were measured after 3 weeks treated with MSC-WJ using the ELISA method. The statistical test used is an independent t-test. The value of p < 0.05 was said to be statistically significant. RESULTS: The result showed that serum MMP-1 levels were higher in the group treated with MSC-WJ than in the control group (p < 0.05). Serum IL-4 levels were higher in the group treated with MSC-WJ than in the control group (p < 0.05). CONCLUSION: This study concluded that MSC-WJ increased MMP-1 levels and IL-4 levels in serum OA rats. MSC-WJ showed a negative effect on MMP-1 in the serum of OA rats.

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Section: Discussionmentioning

confidence: 99%

The Effect of Mesenchymal Stem Cell Wharton's Jelly on Matrix Metalloproteinase-1 and Interleukin-4 Levels in Osteoarthritis Rat Model

Endrinaldi¹,

Darwin²,

Zubir³

et al. 2019

Open Access Maced J Med Sci

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“…The Janus kinase (JAK) family of nonreceptor protein-tyrosine kinases consisting of JAK1, JAK2, JAK3, and JAK1/2 signaling participates in the development of several malignancies including leukemias and lymphomas (Roskoski, 2016). Several research works have reported that JAK2/STAT3 signaling was important in angiogenesis and AML development (Yan et al, 2017;Zhao et al, 2016); hence, it may be a potent target of AML prognosis treatment.…”

Section: Discussionmentioning

confidence: 99%

Wogonoside impedes the progression of acute myeloid leukemia through inhibiting bone marrow angiogenesis

Lin

Zhao

Yang

et al. 2018

Journal Cellular Physiology

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Decreasing bone marrow (BM) microvessel density and circulating angiogenic cytokine levels are promising strategies for the treatment of relapsed and resistant acute myeloid leukemia (AML). Previous studies have reported that wogonoside could inhibit the progression of AML and suppress angiogenesis in a solid tumor, but the correlation of these two effects was ignored. In this research, we determined whether wogonoside could inhibit angiogenesis in this hematologic malignancy. We found that wogonoside could inhibit tumor growth and progression, and prolong the survival of nude mice inoculated with U937/MDR. Besides, reducing BM angiogenesis might cause therapeutic effect against resistant AML. Therefore, coculture between AML cells and BM stromal cells was established to imitate their crosstalk. Then, the effect of wogonoside on BM angiogenesis was tested in vitro and in vivo. We found that wogonoside could suppress microvessel formation in the chicken chorioallantoic membrane assay model and matrigel plug assay. The mechanism research revealed that wogonoside could block the JAK2-STAT3 pathway in AML cells and stromal cells to break their positive feedback. We detected several cytokines related to AML or angiogenesis and found that secreted interleukin-8 was a significant angiogenic cytokine to induce BM angiogenesis. These findings supported that new diagnostics and promising treatment strategies could be developed in relapsed and resistant AML patients.

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Downregulation of MMP1 in MDS-Derived Mesenchymal Stromal Cells Reduces the Capacity to Restrict MDS Cell Proliferation

Cited by 2 publications

References 23 publications

The Effect of Mesenchymal Stem Cell Wharton's Jelly on Matrix Metalloproteinase-1 and Interleukin-4 Levels in Osteoarthritis Rat Model

The Effect of Mesenchymal Stem Cell Wharton's Jelly on Matrix Metalloproteinase-1 and Interleukin-4 Levels in Osteoarthritis Rat Model

Wogonoside impedes the progression of acute myeloid leukemia through inhibiting bone marrow angiogenesis

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