Downregulation of pancreatic-duodenal homeobox 1 expression in breast cancer patients: A mechanism of proliferation and apoptosis in cancer

Ma, Jie; Li, Jia; Li, Hua; Xiao, Xigang; Shen, Lei; Fang, Lin

doi:10.3892/mmr.2012.1067

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…In the third step, we assessed the expression levels of the proliferation marker Ki-67 in our study cohort and observed significant upregulation in HNC tumor tissues compared with adjacent control tissues. A similar pattern of Ki-67 overexpression has been reported in different cancers (33, 34). Jiang et al (35) reported that Ki-67 overexpression is related to increased tumor proliferation, recurrence and metastasis.…”

Section: Discussionsupporting

confidence: 85%

Increased Expression of ERCC2 Gene in Head and Neck Cancer is Associated with Aggressive Tumors: A Systematic Review and Case-Control Study

2016

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Section: Discussionsupporting

confidence: 85%

Increased Expression of ERCC2 Gene in Head and Neck Cancer is Associated with Aggressive Tumors: A Systematic Review and Case-Control Study

2016

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Apoptotic Effect of Koumine on Human Breast Cancer Cells and the Mechanism Involved

Zhang

Chen

Gao

et al. 2015

Cell Biochem Biophys

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Koumine is an alkaloid separated from traditional Chinese herb Gelsemium elegans. In this study, anticancer activity and underlying mechanisms were investigated with an extract using human breast cancer cells. The survival rate was reduced in a concentration- and time-dependent manner as assessed by MTT assay. After incubation for 48 h, typical apoptotic morphological changes were observed by Hoechst 33258 dye assay. Flow cytometry result revealed that the treatment obviously induced G2/M arrest and apoptosis in MCF-7 cells. Furthermore, Western blotting demonstrated the down-regulation of protein expression of Bcl-2, whereas Bax and caspase-3 expressions were up-regulated. Therefore, we propose that koumine has the potential to be a future breast cancer chemotherapeutic agent.

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Deregulation of base excision repair gene expression and enhanced proliferation in head and neck squamous cell carcinoma

et al. 2014

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Defects in the DNA damage repair pathway contribute to cancer. The major pathway for oxidative DNA damage repair is base excision repair (BER). Although BER pathway genes (OGG1, APEX1 and XRCC1) have been investigated in a number of cancers, our knowledge on the prognostic significance of these genes and their role in head and neck squamous cell carcinoma is limited. Protein levels of OGG1, APEX1 and XRCC1 and a proliferation marker, Ki-67, were examined by immunohistochemical analysis, in a cohort of 50 HNSCC patients. Significant downregulation of OGG1 (p<0.04) and XRCC1 (p<0.05) was observed in poorly differentiated HNSCC compared to mod-well-differentiated cases. Significant upregulation of APEX1 (p<0.05) and Ki-67 (p<0.05) was observed in poorly differentiated HNSCC compared to mod-well-differentiated cases. Significant correlation was observed between XRCC1 and OGG1 (r=0.33, p<0.02). Inverse correlations were observed between OGG1 and Ki-67 (r=-0.377, p<0.005), between APEX1 and XRCC1 (r=-0.435, p<0.002) and between OGG1 and APEX1 (r=-0.34, p<0.02) in HNSCC. To confirm our observations, we examined BER pathway genes and a proliferation marker, Ki-67, expression at the mRNA level on 50 head and neck squamous cell carcinoma (HNSCC) and 50 normal control samples by quantitative real-time polymerase chain reaction. Significant downregulation was observed in case of OGG1 (p<0.04) and XRCC1 (p<0.02), while significant upregulation was observed in case of APEX1 (p<0.01) and Ki-67 (p<0.03) in HNSCC tissue samples compared to controls. Our data suggested that deregulation of base excision repair pathway genes, such as OGG1, APEX1 and XRCC1, combined with overexpression of Ki-67, a marker for excessive proliferation, may contribute to progression of HNSCC in Pakistani population.

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Downregulation of pancreatic-duodenal homeobox 1 expression in breast cancer patients: A mechanism of proliferation and apoptosis in cancer

Cited by 3 publications

References 17 publications

Increased Expression of ERCC2 Gene in Head and Neck Cancer is Associated with Aggressive Tumors: A Systematic Review and Case-Control Study

Increased Expression of ERCC2 Gene in Head and Neck Cancer is Associated with Aggressive Tumors: A Systematic Review and Case-Control Study

Apoptotic Effect of Koumine on Human Breast Cancer Cells and the Mechanism Involved

Deregulation of base excision repair gene expression and enhanced proliferation in head and neck squamous cell carcinoma

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