Downregulation of SNHG1 suppresses cell proliferation and invasion by regulating Notch signaling pathway in esophageal squamous cell cancer

Zhang, Yijun; Jin, Xin; Wang, Zhe; Zhang, Xiaokai; Liu, Shen; Li, Gang

doi:10.3233/cbm-170286

Cited by 56 publications

(57 citation statements)

References 21 publications

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“…The results from Yan et al found that SNHG1 was significantly up-regulated in esophageal carcinoma tissues [36]. Zhang et al obtained similar results for SNHG1 in esophageal squamous cell cancer [37]. miR-195 was also proven to be a tumor suppressor in several cancers.…”

Section: Hepg2 Huh7mentioning

confidence: 84%

Long non-coding RNA SNHG1 promotes cell proliferation and invasion of hepatocellular carcinoma by acting as a molecular sponge to modulate miR-195

Zhang¹,

Song²

2020

aoms

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Introduction: Although long non-coding RNA SNHG1 (lncRNA SNHG1) action on cell proliferation and invasion of hepatocellular carcinoma (HCC) cells has been reported, the effects of lncRNA SNHG1 on migration of HCC cells and the mechanisms are still unclear. The present study aimed to investigate the influence of lncRNA SNHG1 on metastasis in HCC cells and the possible mechanisms underlying this phenotype. Material and methods: Expression of lncRNA SNHG1 and miR-195 was determined using qRT-PCR in both HCC cell lines Huh7 and HepG2. Si-RNA was used to silence SNHG1 and miR-195 inhibitor was used to inhibit expression of miR-195. Luciferase reporter assay was conducted to confirm whether miR-195 was the direct binding target of SNHG1. Results: lncRNA SNHG1 was significantly up-regulated and miR-195 was significantly down-regulated in HCC cell lines. When transfected with si-SN-HG1, migration and invasion of HCC cells, as well as expression of astrocyte elevated gene 1 (AEG-1) protein, were significantly inhibited compared with the control cells. Results of dual luciferase reporter assay showed that lncRNA SNHG1 acted as an endogenous sponge of miR-195. On the other hand, the expression of miR-195 in tumor tissue was much lower than that of miR-195 in the corresponding normal tissue. Furthermore, the correlation analysis showed a strong negative relationship between lncRNA SNHG1 and miR-195 expression in HCC tissues. Conclusions: SNHG1 may promote cell invasion and migration in HCC cells by sponging miR-195. These results can provide deeper understanding of SNHG1 in hepatocellular cancer and give new potential targets for treatment of HCC.

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Section: Hepg2 Huh7mentioning

confidence: 84%

Long non-coding RNA SNHG1 promotes cell proliferation and invasion of hepatocellular carcinoma by acting as a molecular sponge to modulate miR-195

Zhang¹,

Song²

2020

aoms

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“…Studies have shown that some snoRNAs play important roles in biological processes, and dysfunction of snoRNAs may lead to oncogenesis [16]. It has also been reported that snoRNAs could serve as biomarkers in several diseases, including cancers [23].…”

Section: Discussionmentioning

confidence: 99%

“…LncRNA SNHG1 may be a potential predictor of prognosis in ESCC patients and a novel target for ESCC treatment [16].…”

Section: Introductionmentioning

confidence: 99%

Identification and analysis of small nucleolar RNAs (snoRNAs) associated co-expression and ceRNA regulatory networks in esophageal carcinoma

Jiang¹,

Zhuang²,

Yang³

et al. 2020

Preprint

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Background: This study aimed to investigate the role of small nucleolar RNAs (snoRNAs) and their host genes (SNHGs) in tumorigenesis and progression of esophageal carcinoma (EC). Methods: RNA-seq data and clinical information for EC patients were obtained from The Cancer Genome Atlas database. Differentially expressed snoRNAs (DE-snoRNAs) and SNHGs (DE-SNHGs) between EC samples and normal controls were screened with the edgeR package, followed by the trend analysis with STEM. Survival analysis was performed using the Kaplan–Meier method with the log-rank test. Identification of co-expressed genes and functional enrichment analyses were performed with Pearson’s correlation analysis and enrichment analysis, respectively. Lastly, the co-expression and ceRNA networks were constructed. Results: A total of 19 DE-snoRNAs and nine DE-SNHGs were identified between EC samples and normal controls. Moreover, two snoRNA clusters related to the clinic stage were identified through trend analysis. Survival analysis results demonstrated that SNORA70D was significantly associated with the overall survival of EC patients (P=0.034). In addition, a co-regulation network that included six snoRNAs and 29 mRNAs was constructed. A ceRNA network comprised of five SNHGs, 11 mRNAs, and 38 miRNAs was constructed based on the top 50 relationship pairs. KEGG pathway enrichment analysis revealed that SNORA14B, SNORA47, SNORA71C, SNORD12B, and SNORD14E in the co-expression network were mainly enriched in the NOD−like receptor signaling pathway, and neuroactive ligand−receptor interaction pathway. SNHG23, SNHG3, SNHG17, SNHG4, and SNHG8 in the ceRNA regulation network were mainly enriched in calcium signaling pathway, cytokine−cytokine receptor interaction, and the extracellular matrix−receptor interaction pathway. Conclusion: The data revealed a series of snoRNAs, SNHGs, and pathways involved in EC carcinogenesis, which will provide a basis for understanding the underlying molecular mechanism of EC development.

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“…With the advances in high throughput sequencing, an increasing number of snoRNAs have been screened and are emerging as important RNAs, thereby attracting the attention of researchers. Studies have shown that some snoRNAs play important roles in biological processes, and dysfunction of snoRNAs may lead to oncogenesis [16]. It has also been reported that snoRNAs could serve as biomarkers in several diseases, including cancers [23].…”

Section: Discussionmentioning

confidence: 99%

“…SNHG8 may play oncogenic roles in the malignancy of ESCC, and offer potential biomarkers for the diagnosis and prognosis of ESCC [15]. LncRNA SNHG1 may be a potential predictor of prognosis in ESCC patients and a novel target for ESCC treatment [16].…”

Section: Introductionmentioning

confidence: 99%

Analysis of small nucleolar RNAs (snoRNAs) associated co-expression and construction of ceRNA regulatory networks in esophageal carcinoma

Jiang

Zhuang

Yang

et al. 2020

Preprint

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Purpose: This study aimed to investigate the role of small nucleolar RNAs (snoRNAs) and their host genes (SNHGs) in tumorigenesis and progression of esophageal carcinoma (EC).Methods: RNA-seq data and clinical information for EC patients were obtained from The Cancer Genome Atlas database. Differentially expressed snoRNAs (DE-snoRNAs) and SNHGs (DE-SNHGs) between EC samples and normal controls were screened with the edgeR package, followed by the trend analysis with STEM. Survival analysis was performed using the Kaplan-Meier method with the log-rank test. Screening of co-expressed genes and functional enrichment analyses were performed with Pearson's correlation analysis and enrichment analysis, respectively. Lastly, the co-expression and ceRNA networks were constructed.Results: A total of 19 DE-snoRNAs and nine DE-SNHGs were screened between EC samples and normal controls. Moreover, two snoRNA clusters related to the clinic stage were analyzed through trend analysis. Survival analysis results demonstrated that SNORA70D was significantly associated with the overall survival of EC patients (P=0.034). In addition, a co-regulation network that included six snoRNAs and 29 mRNAs was constructed. A ceRNA network comprised of five SNHGs, 11 mRNAs, and 38 miRNAs was constructed based on the top 50 relationship pairs. KEGG pathway enrichment analysis revealed that SNORA14B, SNORA47, SNORA71C, SNORD12B, and SNORD14E in the coexpression network were mainly enriched in the NOD−like receptor signaling pathway, and neuroactive ligand−receptor interaction pathway. SNHG23, SNHG3, SNHG17, SNHG4, and SNHG8 in the ceRNA regulation network were mainly enriched in calcium signaling pathway, cytokine−cytokine receptor interaction, and the extracellular matrix−receptor interaction pathway.Conclusion: The data revealed a series of snoRNAs, SNHGs, and pathways involved in EC carcinogenesis, which will provide a basis for understanding the underlying molecular mechanism of EC development. Highlights 1.A series of DE-snoRNAs and DE-SNHGs were screened. 2.Candidate pathways associated with snoRNAs or SNHGs were enriched.3 3. Co-expression and ceRNA networks associated with EC were conducted.

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Downregulation of SNHG1 suppresses cell proliferation and invasion by regulating Notch signaling pathway in esophageal squamous cell cancer

Abstract: These results indicated that lncRNA SNHG1 may be a potential predictor of prognosis in ESCC patients and a novel target for ESCC treatment.

Cited by 56 publications

References 21 publications

Long non-coding RNA SNHG1 promotes cell proliferation and invasion of hepatocellular carcinoma by acting as a molecular sponge to modulate miR-195

Long non-coding RNA SNHG1 promotes cell proliferation and invasion of hepatocellular carcinoma by acting as a molecular sponge to modulate miR-195

Identification and analysis of small nucleolar RNAs (snoRNAs) associated co-expression and ceRNA regulatory networks in esophageal carcinoma

Analysis of small nucleolar RNAs (snoRNAs) associated co-expression and construction of ceRNA regulatory networks in esophageal carcinoma

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