Downregulation of the expression of HDGF attenuates malignant biological behaviors of hilar cholangiocarcinoma cells

Abstract

Hepatoma-derived growth factor (HDGF) has been reported to be a potential predictive and prognostic marker for several types of cancer and important in malignant biological behaviors. However, its role in human hilar cholangiocarcinoma remains to be elucidated. Our previous study demonstrated that high expression levels of HDGF in hilar cholangiocarcinoma tissues correlates with tumor progression and patient outcome. The present study aimed to elucidate the detailed functions of the HDGF protein. This was performed by downregulating the protein expression of HDGF in the FRH0201 hilar cholangiocarcinoma cell line by RNA interference (RNAi) in vitro, and revealed that downregulation of the HDGF protein significantly inhibited the malignant biological behavior of the FRH0201 cells. In addition, further investigation revealed that downregulation of the protein expression of HDGF significantly decreased the secretion of vascular endothelial growth factor, which may be the mechanism partially responsible for the inhibition of malignant biological behaviors. These findings demonstrated that HDGF is important in promoting malignant biological behaviors, including proliferation, migration and invasion of hilar cholangiocarcinoma FRH0201 cells. Inhibition of the expression of HDGF downregulated the malignant biological behaviors, suggesting that downregulation of the protein expression of HDGF by RNAi may be a novel therapeutic approach to inhibit the progression of hilar cholangiocarcinoma.

HDGF and PRKCA upregulation is associated with a poor prognosis in patients with lung adenocarcinoma

“…Studies have demonstrated that high HDGF expression is a novel prognostic factor in several types of tumour, including gall bladder cancer (32), endometrial carcinoma (33) and cholangiocarcinoma (34). In the present study, HDGF was found to be highly expressed in LADC tissues by immunohistochemical staining, and these levels were associated with N classification, lymph node metastasis and AJCC clinical stage.…”

Unraveling molecular effects of ADAR1 overexpression in HEK293T cells by label-free quantitative proteomics

“…33 Thus, it is consistent that NUDC upregulation is associated with cell proliferation in ADAR1 over-expressed cells. Finally, HDGF is reported to be a predictive and prognostic marker for several types of cancer 34 and overexpressed in ovarian cancer cell lines. 35 HDGF is also shown to stimulate proliferation of hepatoma cells in vitro.…”