Downregulation of TRIM27 expression inhibits the proliferation of ovarian cancer cells in vitro and in vivo

Abstract

(tripartite motif-containing 27) was originally identified as a fusion partner with the RET (REarranged during transfection) proto-oncogene and is highly expressed in various tumor cells and tissues. However, the level of expression and function of TRIM27 in ovarian cancer remain unclear. Here we have measured the expression of TRIM27 in normal ovarian and fallopian tube epithelial cells and in ovarian serous carcinoma cells and correlated TRIM27 expression with clinical and pathological parameters. In addition, we detected the effect of TRIM27 knockdown on proliferation of ovarian cancer cells in cell culture and xenografts. The results demonstrated that TRIM27 was highly expressed in ovarian serous carcinoma cells, and TRIM27 expression was significantly correlated with metastasis and FIGO stage in ovarian serous carcinoma patients. Downregulation of TRIM27 expression suppressed the proliferation of ovarian cancer cells in cell culture and inhibited the growth of xenografts in nude mice. TRIM27 knockdown induced cell cycle arrest and apoptosis in ovarian cancer cells by upregulating the expression of p-P38 and downregulating the expression of p-AKT. Thus the present study suggests that TRIM27 could have important roles as an oncogene during the development of ovarian cancer and could serve as a diagnostic and therapeutic target.

Knockdown of TRIM27 expression suppresses the dysfunction of mesangial cells in lupus nephritis by FoxO1 pathway

“…However, whether TRIM27 participates in the progression of lupus nephritis, especially in cell proliferation is still unknown. In this study, we first revealed that the (Ma et al, 2015). Similarly, consistent with these literature, we discovered the overexpression of TRIM27 in the (Gendelman et al, 2017).…”

“…Several articles had reported that TRIM27 expression played an important role in the proliferation of carcinoma cells. For instance, Ma et al found TRIM27 expression was increased in ovarian serous carcinoma cells and downregulation of TRIM27 expression induced cell cycle arrest (Ma et al, ). Similarly, consistent with these literature, we discovered the overexpression of TRIM27 in the kidneys of patients with LN relating to the proliferation of mesangial cells.…”

miR-30b-3p Inhibits Proliferation and Invasion of Hepatocellular Carcinoma Cells via Suppressing PI3K/Akt Pathway

“…Previous studies reported that TRIM27 functioned in an oncogenic role in colorectal cancer and ovarian cancer (Ma et al, 2016). However, the role of TRIM27 in HCC remains unclear.…”

Proliferation and invasion of ovarian cancer cells are suppressed by knockdown of TRIM11

“…Recently, it has been reported that TRIM11 is upregulated and play an oncogenic function in glioma (8), lung cancer (9) and colon cancer (10). Several members of TRIM proteins, including TRIM25 (11), TRIM27 (12) and TRIM29 (13), were elevated in ovarian cancer, while TRIM16 expression (14) decreased in ovarian cancer. However, the expression, and functions of TRIM11 in ovarian cancer have been insufficiently characterized.…”