Downregulation of TXNIP leads to high proliferative activity and estrogen-dependent cell growth in breast cancer

Lee, Su Hyung; Woo, Gye-Hyung; Kwon, Hyo Jung; Kim, Dae-Yong

doi:10.1016/j.bbrc.2018.03.020

Cited by 49 publications

(34 citation statements)

References 21 publications

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“…Additionally, TXNIP stabilizes the Cdk inhibitors p16 and p27, which inhibit cell division and keep the cells in G 1 25,26 . These observations place TXNIP in the category of cell cycle inhibitors, a role which is supported by studies showing that TXNIP down-regulation is needed for cell division 27,28 , and that TXNIP functions as a tumor suppressor 19,29–31 . TXNIP expression is strongly and acutely regulated at the transcriptional level by several transcriptions factors, including MondoA, Mlx, FOXO1, and ChREBP 32 .…”

Section: Introductionmentioning

confidence: 70%

“…Previous studies have found that TNF can induce TXNIP down-regulation in macrophages and cell lines 30,33,34 , and that TXNIP acts as an inhibitor of glucose uptake, tumor cell proliferation and cell cycle progression 26,30,31,41 . To determine whether TNF affects TXNIP expression in human T cells, we purified T cells from healthy donors, incubated them for 4 h in the absence or presence of TNF and subsequently determined their TXNIP expression levels by Western blot analyses.…”

Section: Resultsmentioning

confidence: 98%

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Tumor necrosis factor induces rapid down-regulation of TXNIP in human T cells

Levring

Kongsbak-Wismann

Rode

et al. 2019

Sci Rep

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In addition to antigen-driven signals, T cells need co-stimulatory signals for robust activation. Several receptors, including members of the tumor necrosis factor receptor superfamily (TNFRSF), can deliver co-stimulatory signals to T cells. Thioredoxin interacting protein (TXNIP) is an important inhibitor of glucose uptake and cell proliferation, but it is unknown how TXNIP is regulated in T cells. The aim of this study was to determine expression levels and regulation of TXNIP in human T cells. We found that naïve T cells express high levels of TXNIP and that treatment of blood samples with TNF results in rapid down-regulation of TXNIP in the T cells. TNF-induced TXNIP down-regulation correlated with increased glucose uptake. Furthermore, we found that density gradient centrifugation (DGC) induced down-regulation of TXNIP. We demonstrate that DGC induced TNF production that paralleled the TXNIP down-regulation. Treatment of blood with toll-like receptor (TLR) ligands induced TNF production and TXNIP down-regulation, suggesting that damage-associated molecular patterns (DAMPs), such as endogenous TLR ligands, released during DGC play a role in DGC-induced TXNIP down-regulation. Finally, we demonstrate that TNF-induced TXNIP down-regulation is dependent on caspase activity and is caused by caspase-mediated cleavage of TXNIP.

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Section: Introductionmentioning

confidence: 70%

Section: Resultsmentioning

confidence: 98%

Tumor necrosis factor induces rapid down-regulation of TXNIP in human T cells

Levring

Kongsbak-Wismann

Rode

et al. 2019

Sci Rep

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“…In pancreatic tumor cells, miR-224 could enhance the proliferation and migration via directly targeting TBP-2 and activating downstream hypoxia-inducible factor-1 (HIF-1) signaling pathway (19). Park et al found that downregulation of TBP-2 promoted the tumor growth by reducing p27 expression in vitro and in vivo in breast cancer (20). Interestingly, the bromodomain-containing protein 4 (BRD4) inhibitor, JQ1, induced apoptosis in acute myeloid leukemia cells by reactivating TBP-2 (21).…”

Section: Discussionmentioning

confidence: 99%

miR-93, miR-373, and miR-17-5p Negatively Regulate the Expression of TBP2 in Lung Cancer

Liang

Zhang

et al. 2020

Front. Oncol.

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Recently, several miRNAs have been revealed to play critical roles in oncogenesis and tumor progression of many cancers. Thioredoxin-1 (Trx-1) binding protein-2 (TBP-2) is an internal inhibitor of Trx-1, which plays the role in regulating oxidative stress, inhibiting cell growth, and promoting apoptosis. The expression of TBP-2 is usually decreased in cancer tissues. However, whether the miRNAs regulate the TBP-2 expression in lung cancer is still unclear. In this study, we examined the levels of TBP-2, miR-93, miR-373, and miR-17-5p in lung cancer tissues and their adjacent normal lung tissues of 36 patients. We found that the expressions of miR-93, miR-373, and miR-17-5p were higher, whereas the expression of TBP-2 mRNA and protein was significantly lower in lung cancer tissues compared with adjacent normal lung tissues. After the three miRNA mimics were transfected in the lung cancer cells, NCI-H460, the level of TBP-2 mRNA and TBP-2 protein was decreased. Then, the anti-cancer drug 5-fluorouracil was used to stimulate the NCI-H460 cells; the mRNA levels of miR-93, miR-373, and miR-17-5p were decreased, and the level of TBP-2 mRNA and protein was increased. Collectively, the above results suggest that miR-93, miR-373, and miR-17-5p negatively regulate the TBP-2 expression in lung cancer. This study may provide therapeutic targets with lung cancer.

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“…Further, TXNIP has been found to affect cellular processes ranging from lipid and glucose metabolism to transcription, promote apoptosis and inflammation, and inhibit cellular proliferation. Indeed, due to inhibitory effects on cell growth, TXNIP is classified as a tumor suppressor [42,47], and downregulation of TXNIP expression in a variety of human cancers, including B cell lymphoma, has been reported [66][67][68]. TXNIP expression is known to be regulated by a variety of factors, including cellular stresses, glucose, and cytokines [69][70][71].…”

Section: Discussionmentioning

confidence: 99%

Molecular analysis of lymphoid tissue from rhesus macaque rhadinovirus-infected monkeys identifies alterations in host genes associated with oncogenesis

Estep¹,

Govindan²,

Manoharan³

et al. 2020

PLoS ONE

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Rhesus macaque (RM) rhadinovirus (RRV) is a simian gamma-2 herpesvirus closely related to human Kaposi's sarcoma-associated herpesvirus (KSHV). RRV is associated with the development of diseases in simian immunodeficiency virus (SIV) co-infected RM that resemble KSHV-associated pathologies observed in HIV-infected humans, including B cell lymphoproliferative disorders (LPD) and lymphoma. Importantly, how de novo KSHV infection affects the expression of host genes in humans, and how these alterations in gene expression affect viral replication, latency, and disease is unknown. The utility of the RRV/ RM infection model provides a novel approach to address these questions in vivo, and utilizing the RRV bacterial artificial chromosome (BAC) system, the effects of specific viral genes on host gene expression patterns can also be explored. To gain insight into the effects of RRV infection on global host gene expression patterns in vivo, and to simultaneously assess the contributions of the immune inhibitory viral CD200 (vCD200) molecule to host gene regulation, RNA-seq was performed on pre-and post-infection lymph node (LN) biopsy samples from RM infected with either BAC-derived WT (n = 4) or vCD200 mutant RRV (n = 4). A variety of genes were identified as being altered in LN tissue samples due to RRV infection, including cancer-associated genes activation-induced cytidine deaminase (AICDA), glypican-1 (GPC1), CX3C chemokine receptor 1 (CX3CR1), and Ras dexamethasone-induced 1 (RasD1). Further analyses also indicate that GPC1 may be associated with lymphomagenesis. Finally, comparison of infection groups identified the differential expression of host gene thioredoxin interacting protein (TXNIP), suggesting a possible mechanism by which vCD200 negatively affects RRV viral loads in vivo.

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Downregulation of TXNIP leads to high proliferative activity and estrogen-dependent cell growth in breast cancer

Cited by 49 publications

References 21 publications

Tumor necrosis factor induces rapid down-regulation of TXNIP in human T cells

Tumor necrosis factor induces rapid down-regulation of TXNIP in human T cells

miR-93, miR-373, and miR-17-5p Negatively Regulate the Expression of TBP2 in Lung Cancer

Molecular analysis of lymphoid tissue from rhesus macaque rhadinovirus-infected monkeys identifies alterations in host genes associated with oncogenesis

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