Downstream Processing for Isomaltose following a Reaction Integrated Adsorption

Abstract

Downstream processing for isomaltose by purification on BEA-zeolites was investigated. The important process steps are washing procedure, desorption, and reactivation of the adsorbent. Purity and yield were influenced by the enzyme system. Desorption kinetics was determined at 50°and 70°C. Complete desorption was performed in a three stage cross-flow-sequence. The reactivation of BEA-zeolite is feasible with additional effort for Na + -cation exchange.

Continuous enzymatic production and adsorption of laminaribiose in packed bed reactors

“…Besides the use of zeolites in chromatographic columns for sugar separation [11], type BEA zeolites have shown a high selectivity between different carbohydrates [12] and have already been used to capture the disaccharide isomaltose in enzymatic reactions [13][14][15][16]. Successful combination of enzymatic production and adsorption with subsequent downstream processing has only been shown for a monoenzymatic production system where enzymatic catalysis and adsorption were integrated in a set-up comprising of fluidized bed of biocatalysts and a slurry of zeolite particles [16,20]. Successful combination of enzymatic production and adsorption with subsequent downstream processing has only been shown for a monoenzymatic production system where enzymatic catalysis and adsorption were integrated in a set-up comprising of fluidized bed of biocatalysts and a slurry of zeolite particles [16,20].…”

“…Moreover, commercially available BEA 150 zeolite extrudates have shown advantageous adsorption isotherms for the separation of laminaribiose against fructose and glucose as needed here [17,18] whereas sucrose adsorbs even weaker than the solvent water [19]. Successful combination of enzymatic production and adsorption with subsequent downstream processing has only been shown for a monoenzymatic production system where enzymatic catalysis and adsorption were integrated in a set-up comprising of fluidized bed of biocatalysts and a slurry of zeolite particles [16,20]. In here, a bienzymatic production of laminaribiose is combined with adsorption in a system comprising of two solid phases (biocatalyst pearls and zeolite particles) and one fluid phase of reaction solution implemented in two consecutive fixed beds.…”