Downy mildew is a severe disease of cucumber worldwide. The oomycete Pseudoperonospora cubensis cause it and once it is established in a region, the infection spreads rapidly, causing significant loss of yield and fruit quality. The objective of the research was to develop an ecological strategy for the control of downy mildew in cucumber. The treatments were arranged in a completely randomized experimental design with an alternation of chemical and biological fungicides. The treatments were: T1: systemic fungicide (Ridomil Gold, 2.5 g/l) alternating with a contact fungicide (Bravo 2.5 ml/l), T2: CustomBio 5 (Bacillus-based fungicide, 3ml/l), T3: control (water), T4: Trichoderma sp. (3 ml/l), T5: systemic fungicide (Ridomil Gold 2.5g/l) alternating with CustomBio 5 (3 ml/l), and T6: systemic fungicide (Ridomil Gold 2.5g/l) alternating with Trichoderma sp. (3 ml/l). The following variables were evaluated: stem thickness, plant height, number and weight of fruits, yield, the area under the relative progress curve (AUDPCr), and economic analysis of each treatment. The results showed that the best treatments were T1 and T6, with an AUDPCr of 11.89% and 12.10%, respectively. Treatments T6 and T1 showed the best yield, as well. The profitability analysis showed that all the alternatives were profitable with a Benefit/Cost>1 ratio. However, the treatments T6 and T1 were the most useful. We recommend this control strategy to reduce the use of chemical fungicides and, at the same time, obtain an efficient control of the disease, which guarantees a significant yield of high-quality fruit.