Doxorubicin and menadione decrease cell proliferation of Saccharomyces cerevisiae by different mechanisms

Saenko, Yury; Shutov, A. M.; Rastorgueva, Eugenia

doi:10.1134/s1990519x1004005x

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…S. cerevisiae as a broadly used model organism for anthracycline drug testing. Observations from other studies illustrate the higher resistance of S. cerevisiae against doxorubicin and the protective effect of glutathione and or metallothioneins [ 70 ]. The assessment of the results should be considered the phase of the cell cycle, which has a direct effect on the toxicity of doxorubicin [ 71 , 72 , 73 , 74 ].…”

Section: Discussionmentioning

confidence: 99%

Zinc-Modified Nanotransporter of Doxorubicin for Targeted Prostate Cancer Delivery

et al. 2017

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This work investigated the preparation of chitosan nanoparticles used as carriers for doxorubicin for targeted cancer delivery. Prepared nanocarriers were stabilized and functionalized via zinc ions incorporated into the chitosan nanoparticle backbone. We took the advantage of high expression of sarcosine in the prostate cancer cells. The prostate cancer targeting was mediated by the AntiSar antibodies decorated surface of the nanocage. Formation of the chitosan nanoparticles was determined using a ninhydrin assay and differential pulse voltammetry. Obtained results showed the strong effect of tripolyphosphine on the nanoparticle formation. The zinc ions affected strong chitosan backbone coiling both in inner and outer chitosan nanoparticle structure. Zinc electrochemical signal depended on the level of the complex formation and the potential shift from −960 to −950 mV. Formed complex is suitable for doxorubicin delivery. It was observed the 20% entrapment efficiency of doxorubicin and strong dependence of drug release after 120 min in the blood environment. The functionality of the designed nanotransporter was proven. The purposed determination showed linear dependence in the concentration range of Anti-sarcosine IgG labeled gold nanoparticles from 0 to 1000 µg/mL and the regression equation was found to be y = 3.8x − 66.7 and R2 = 0.99. Performed ELISA confirmed the ability of Anti-sarcosine IgG labeled chitosan nanoparticles with loaded doxorubicin to bind to the sarcosine molecule. Observed hemolytic activity of the nanotransporter was 40%. Inhibition activity of our proposed nanotransporter was evaluated to be 0% on the experimental model of S. cerevisiae. Anti-sarcosine IgG labeled chitosan nanoparticles, with loaded doxorubicin stabilized by Zn ions, are a perspective type of nanocarrier for targeted drug therapy managed by specific interaction with sarcosine and metallothionein for prostate cancer.

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Section: Discussionmentioning

confidence: 99%

Zinc-Modified Nanotransporter of Doxorubicin for Targeted Prostate Cancer Delivery

et al. 2017

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“…S . cerevisiae has been used to study the effects of doxorubicin on growth inhibition 24 , on cell cycle 25 , on DNA double-strand breaks 26 and on environment-dependent protein complex dynamics 27 . Genome-wide screening with haploid and diploid S .…”

Section: Introductionmentioning

confidence: 99%

Doxorubicin induces an extensive transcriptional and metabolic rewiring in yeast cells

Taymaz-Nikerel

Karabekmez

Eraslan

et al. 2018

Sci Rep

134

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Doxorubicin is one of the most effective chemotherapy drugs used against solid tumors in the treatment of several cancer types. Two different mechanisms, (i) intercalation of doxorubicin into DNA and inhibition of topoisomerase II leading to changes in chromatin structure, (ii) generation of free radicals and oxidative damage to biomolecules, have been proposed to explain the mode of action of this drug in cancer cells. A genome-wide integrative systems biology approach used in the present study to investigate the long-term effect of doxorubicin in Saccharomyces cerevisiae cells indicated the up-regulation of genes involved in response to oxidative stress as well as in Rad53 checkpoint sensing and signaling pathway. Modular analysis of the active sub-network has also revealed the induction of the genes significantly associated with nucleosome assembly/disassembly and DNA repair in response to doxorubicin. Furthermore, an extensive re-wiring of the metabolism was observed. In addition to glycolysis, and sulfate assimilation, several pathways related to ribosome biogenesis/translation, amino acid biosynthesis, nucleotide biosynthesis, de novo IMP biosynthesis and one-carbon metabolism were significantly repressed. Pentose phosphate pathway, MAPK signaling pathway biological processes associated with meiosis and sporulation were found to be induced in response to long-term exposure to doxorubicin in yeast cells.

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Multifunctional Magnetic Nanocomposites on the Base of Magnetite and Hydroxyapatite for Oncology Applications

Pylypchuk

Абрамов

Petranovska

et al. 2018

Springer Proceedings in Physics

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Doxorubicin and menadione decrease cell proliferation of Saccharomyces cerevisiae by different mechanisms

Cited by 3 publications

References 23 publications

Zinc-Modified Nanotransporter of Doxorubicin for Targeted Prostate Cancer Delivery

Zinc-Modified Nanotransporter of Doxorubicin for Targeted Prostate Cancer Delivery

Doxorubicin induces an extensive transcriptional and metabolic rewiring in yeast cells

Multifunctional Magnetic Nanocomposites on the Base of Magnetite and Hydroxyapatite for Oncology Applications

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