Doxorubicin can penetrate nitrile gloves and induces apoptosis in keratinocytes cell lines

Boccellino, Mariarosaria; Pedata, Paola; Castiglia, Loredana; Porta, Raffaele La; Pieri, Maria; Quagliuolo, Lucio; Acampora, Antonio; Sannolo, Nicola; Miraglia, Nadia

doi:10.1016/j.toxlet.2010.04.026

Cited by 16 publications

(21 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many studies have documented surface contamination with these agents in healthcare institutions [10][11][12][13][14] and a recent study noted that doxorubicin can penetrate nitrile gloves. 15 Additionally, hazardous drugs have been found in the urine of healthcare workers who prepare or administer chemotherapy. 11,13,16 PPE is therefore used during preparation and administration in order to reduce exposure during these times.…”

Section: Introductionmentioning

confidence: 99%

Use of a closed system drug-transfer device eliminates surface contamination with antineoplastic agents

Clark

Sessink

2013

J Oncol Pharm Pract

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Use of a closed system drug-transfer device eliminates surface contamination with antineoplastic agents

Clark

Sessink

2013

J Oncol Pharm Pract

View full text Add to dashboard Cite

show abstract

“…; Boccellino et al . ). Although being more comfortable, easier to adjust to hand and providing a good protection, nitrile gloves are less used because of their higher cost.…”

Section: Resultsmentioning

confidence: 97%

“…Disparities were found in the type of glove used, depending on both the unit and the person, with 89% of the units using the vinyl gloves which are the less protective ones, 78% using latex gloves, and 44% using nitrile gloves when handling cytotoxic drugs. Latex and nitrile gloves have been reported to provide the best protection level (Laidlaw et al 1984;Wallemacq et al 2006;Boccellino et al 2010). Although being more comfortable, easier to adjust to hand and providing a good protection, nitrile gloves are less used because of their higher cost.…”

Section: Personal Protective Equipments (Ppe)mentioning

confidence: 99%

Preventing the contamination of hospital personnel by cytotoxic agents: evaluation and training of the para-professional healthcare workers in oncology units

Kieffer

Verhaeghe

Lagrassa

et al. 2014

Eur J Cancer Care (Engl)

View full text Add to dashboard Cite

This study was specifically focused on para-professional healthcare workers (PHCWs) who handle cytotoxic drugs and contaminated wastes at the Public Teaching Hospitals of Marseille (AP-HM), France. It first aimed at evaluating the knowledge and professional practice of the PHCWs who belong to a personnel category among the less informed and protected in hospitals. In a second time, this study also proposed to raise awareness, educate and train the staff on protective measures to minimise the exposure of the PHCWs to the potential toxicity of anticancer chemotherapy agents (or metabolites) when cleaning and handling both cytotoxic drugs and wastes. Among the 11 oncology units evaluated, 82% completed an assessment survey, 63% of which were PHCWs. Out of nine oncology units assessed, 89% reported limited knowledge of the general risk and of the safe handling of cytotoxic drugs, 89% reported using vinyl gloves which are the less protective ones. Forty-four per cent of the units used wet sweeping techniques for cleaning the floors, and 11% of the units did not have specific procedures for cleaning the equipments used for collecting contaminated excreta. Protective outer apparel was not always worn and chemotherapy wastes were not managed consistently between all units. Standardized procedures and guidelines to prevent occupational exposure were not used by PHCWs. More education and training are needed to improve safety.

show abstract

“…Permeability experiments were performed under static conditions, as previously reported. 16 Briefly, for each of the four different glove types, four sets of two gloves were challenged with 1 ml of 2 mg/ml EPI physiologic or acid solution. EPI solutions were left in contact with each gloves set for different contact times: 0 (reference starting point: gloves were immediately analyzed), 0.5, 1 and 8 h. Then, for each glove set, the first glove was used for the external side wiping, while the second one was wiped after having been turned (internal side).…”

Section: Permeability Experimentsmentioning

confidence: 99%

“…23--25 Even less are the scientific studies investigating gloves permeability to chemicals handled in occupational contexts different from hospitals' drugs preparation rooms, such as occupational medicine laboratories and/or of synthesis industries. 16 In particular, Boccellino et al 16 focused the attention on doxorubicin, an anthracycline antibiotic, extensively used in oncological therapies and performed permeation experiments at different pH, highlighting the crucial role of such parameter on gloves' performances. Doxorubicin long-term clinical use is limited by the development of a cumulative dose-related cardiotoxicity and because of its probable carcinogenicity to humans.…”

Section: Introductionmentioning

confidence: 99%

Epirubicin permeation of personal protective equipment can induce apoptosis in keratinocytes

Pieri

Quagliuolo

Porta

et al. 2012

J Expo Sci Environ Epidemiol

Self Cite

View full text Add to dashboard Cite

The present study investigated the epirubicin (EPI) permeability of various commercially available glove types, as well as toxicity mechanisms and effects on human keratinocyte cell line (HaCaT). Permeability experiments were carried out on various commercially available gloves, differing as regards material and thickness. Permeability was evaluated after different "contact times" and the influence of EPI solution's pH (acid and neutral) on permeability was also examined. Toxicity of EPI toward skin was tested by evaluating the effects of the drug on cell growth and apoptosis, by using an in vitro model based on cultured immortalized human keratinocytes. No permeation was detected in the case of EPI neutral solutions; in contrast, acid solutions were found to penetrate low thickness nitrile gloves. Obtained results also showed the induction of apoptosis in epithelial cells through the activation of intrinsic pathway p53-independent occurring even when cells are exposed at low drug concentration. EPI solution's pH influences the glove's permeability; once penetrated, EPI at concentrations lower than those able to penetrate the nitrile glove during the 8-h work-shift can cause apoptosis in epithelial cells. The findings reported here highly support the choice of either natural rubbers gloves or high thickness nitrile ones for preventing the occupational exposure to EPI.

show abstract

Doxorubicin can penetrate nitrile gloves and induces apoptosis in keratinocytes cell lines

Cited by 16 publications

References 32 publications

Use of a closed system drug-transfer device eliminates surface contamination with antineoplastic agents

Use of a closed system drug-transfer device eliminates surface contamination with antineoplastic agents

Preventing the contamination of hospital personnel by cytotoxic agents: evaluation and training of the para-professional healthcare workers in oncology units

Epirubicin permeation of personal protective equipment can induce apoptosis in keratinocytes

Contact Info

Product

Resources

About